Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF‐7 in vitro

Abstract

Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients. Their proliferation, differentiation, immunophenotype and gene expression were assessed and effects on the proliferation of human breast cancer cell line MCF-7 compared. ASCs from all sources exhibited similar morphology, proliferative and differentiation potential, showing the characteristic pattern of mesenchymal surface markers expression (CD90, CD105, CD44H, CD73) and the lack of HLA-DR and hematopoietic markers (CD11a, CD33, CD45, Glycophorin-CD235a), but uneven expression of CD34. ASCs also shared a common positive gene expression of HLA-DR, HLA-A, IL-6, TGF-β and HIF-1, but were negative for HLA-G, while the expression levels of Cox-2 and IDO-1 varied. All ASCs significantly stimulated the proliferation of MCF-7 tumour cells in direct mixed co-cultures and transwell system, although their conditioned media displayed antiproliferative activity. Data obtained showed that ASCs with similar characteristics are easily isolated from various donors and sites of origin, although ASCs could both suppress and favour tumour cells growth, emphasising the importance of cellular context within the microenvironment and pointing to the significance of safety studies to exclude any potential clinical risk of their application in regenerative medicine.

Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression

“…The pellets were resuspended in lysis buffer (154 mM NH 4 Cl) and incubated at room temperature for 10 min to lyse erythrocytes. Cells were seeded and cultured in 25 cm 2 culture flask in GM under standard conditions until colonies appeared (Trivanović et al, 2014).…”

Adipose-Derived Stems Cells and Their Role in Human Cancer Development, Growth, Progression, and Metastasis: A Systematic Review

“…Other authors have reported that ASCs may be protective, particularly in suppressing tumor growth and stimulating apoptosis. Most of the articles reviewed consistently pointed out that the role of ASCs in tumor development is complex, and that the tumor microenvironment milieu in which ASCs are present and the mechanisms by which they migrate may influence their role in cancer development (41)(42)(43)(44)(45).…”

“…Several mechanisms linking ASCs and tumor development/ progression have been described, with many studies highlighting the importance of tumor environment modification by ASCs (41)(42)(43)(44). ASCs may play a role in the creation of an inflammatory tumor microenvironment, which promotes tumorigenic activity.…”

The Roles of Mesenchymal Stromal/Stem Cells in Tumor Microenvironment Associated with Inflammation

“…For the same reasons, MSCs have received great attention in cancer research. It has been proposed that normal MSCs (N-MSCs) and tumor MSCs (T-MSCs), although sharing similar phenotype properties, could have different influence on tumor development, demonstrating supportive role of T-MSCs in tumor growth [43, 44]. Aforementioned data indicated conspicuous importance of TME not only in tumor development, but also in cancer recurrence and poor prognosis of patients.…”