Abstract:A heterotrophic nitrification and aerobic denitrification bacterium, strain D51, was identified as Arthrobacter nicotianae based on morphological, phospholipid fatty acids (PLFAs), and 16S rRNA gene sequence analyses. Further tests demonstrated that strain D51 had the capability to use nitrite, nitrate, or ammonium as the sole nitrogen source in the presence of Cu2+. The maximum removal efficiencies of nitrite, nitrate and ammonium were 68.97%, 78.32%, and 98.70%, respectively. Additionally, the maximum growth… Show more
“…was observed in the presence of Cr 6+ stress (10 mgL −1 ) (Sun et al 2016). Similarly, Cu 2+ at a concentration > 0.1 mgL −1 inhibited growth and denitrification by Arthrobacter nicotianae (Cai et al 2019). Thus, strain ARB2 can be used in nitrate removal from wastewaters where salt and metal concentrations are low or within permissible limits.…”
Denitrification is a potential strategy for nitrate removal from wastewater. This study reports the isolation of a novel bacterium Georgenia daeguensis ARB2 which has not been earlier reported for treatment of wastewater. ARB2 was isolated from pharmaceutical wastewater and optimized for growth and nitrate removal under conditions of 10 mM nitrate stress (140 mgL −1 NO − 3 − N ). It can utilize nitrate as a sole source of nitrogen, exhibits highest growth and aerobic denitrification using glycogen + maltose as a carbon source at pH 7.0, carbon-to-nitrogen ratio of 34, temperature 30 °C, and shaking speed of 100 rpm. ARB2 removes 20% of the initial nitrate concentration of 10 mM in 56 h, and 40% of the initial nitrate concentration of 1 mM in 52 h. Further, the efficacy of strain ARB2 was tested on real contaminated waters and was found to successfully reduce nitrate levels in them. The findings suggest that Georgenia daeguensis ARB2 has a potential application for the alleviation of nitrate under aerobic conditions.
“…was observed in the presence of Cr 6+ stress (10 mgL −1 ) (Sun et al 2016). Similarly, Cu 2+ at a concentration > 0.1 mgL −1 inhibited growth and denitrification by Arthrobacter nicotianae (Cai et al 2019). Thus, strain ARB2 can be used in nitrate removal from wastewaters where salt and metal concentrations are low or within permissible limits.…”
Denitrification is a potential strategy for nitrate removal from wastewater. This study reports the isolation of a novel bacterium Georgenia daeguensis ARB2 which has not been earlier reported for treatment of wastewater. ARB2 was isolated from pharmaceutical wastewater and optimized for growth and nitrate removal under conditions of 10 mM nitrate stress (140 mgL −1 NO − 3 − N ). It can utilize nitrate as a sole source of nitrogen, exhibits highest growth and aerobic denitrification using glycogen + maltose as a carbon source at pH 7.0, carbon-to-nitrogen ratio of 34, temperature 30 °C, and shaking speed of 100 rpm. ARB2 removes 20% of the initial nitrate concentration of 10 mM in 56 h, and 40% of the initial nitrate concentration of 1 mM in 52 h. Further, the efficacy of strain ARB2 was tested on real contaminated waters and was found to successfully reduce nitrate levels in them. The findings suggest that Georgenia daeguensis ARB2 has a potential application for the alleviation of nitrate under aerobic conditions.
“…An increase in copper concentration from 0·01, to 0·1 mmol l −1 resulted in decreased denitrification from 99·8 to 16·3% (Lu et al ). A study conducted by Cai et al () showed the growth and denitrification ability of Arthrobater nicotianae in the presence of Cu 2+ . Cu 2+ at a concentration of >0·1 mg l −1 inhibited both growth and denitrification.…”
Section: Aerobic Denitrification In the Presence Of Metal Ionsmentioning
With the increase in industrial and agricultural activities, a large amount of nitrogenous compounds are released into the environment, leading to nitrate pollution. The perilous effects of nitrate present in the environment pose a major threat to human and animal health. Bioremediation provides a costeffective and environmental friendly method to deal with this problem. The process of aerobic denitrification can reduce nitrate compounds to harmless dinitrogen gas. This review provides a brief view of the exhaustive role played by aerobic denitrifiers for tackling nitrate pollution under different ecological niches and their dependency on various environmental parameters. It also provides an understanding of the enzymes involved in aerobic denitrification. The role of aerobic denitrification to solve the issues faced by the conventional method (aerobic nitrification-anaerobic denitrification) in treating nitrogenpolluted wastewaters is elaborated. verts the iron centre of haemoglobin from Fe 2+ to Fe 3+
Role of heterotrophic aerobic denitrifying bacteriaA. Rajta et al.
“…Many members of the genus Arthrobacter have been found to have the ability to reduce nitrate, such as Arthrobacter oryzae , Arthrobacter pokkalii , Arthrobacter globiformis , Arthrobacter arilaitensis ( Pinar and Ramos, 1997 ; Kageyama et al, 2008 ; He and Li, 2016 ; Krishnan et al, 2016 ). And it has been shown that the reduction of nitrate by Arthrobacter is usually considered to be through denitrification ( He et al, 2017 ; Zhong et al, 2018 ; Cai et al, 2019 ). However, nitrate reduction to ammonium by Arthrobacter has rarely been reported.…”
According to average nucleotide identity (ANI) analysis of the complete genomes, strain 24S4–2 isolated from Antarctica is considered as a potential novel Arthrobacter species. Arthrobacter sp. 24S4–2 could grow and produce ammonium in nitrate or nitrite or even nitrogen free medium. Strain 24S4–2 was discovered to accumulate nitrate/nitrite and subsequently convert nitrate to nitrite intracellularly when incubated in a nitrate/nitrite medium. In nitrogen-free medium, strain 24S4–2 not only reduced the accumulated nitrite for growth, but also secreted ammonia to the extracellular under aerobic condition, which was thought to be linked to nitrite reductase genes nirB, nirD, and nasA by the transcriptome and RT-qPCR analysis. A membrane-like vesicle structure was detected in the cell of strain 24S4–2 by transmission electron microscopy, which was thought to be the site of intracellular nitrogen supply accumulation and conversion. This spatial and temporal conversion process of nitrogen source helps the strain maintain development in the absence of nitrogen supply or a harsh environment, which is part of its adaption strategy to the Antarctic environment. This process may also play an important ecological role, that other bacteria in the environment would benefit from its extracellular nitrogen source secretion and nitrite consumption characteristics.
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