Characteristics of Guinea Pig IgE

Óváry, Zoltán; Kaplan, B.; Kojima, Satoshi

doi:10.1159/000231616

Cited by 73 publications

(38 citation statements)

References 15 publications

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“…According to the method of Ovary et al (13) with slight modifications, the Ig Gl and Ig E levels in the serum of each guinea pig were estimated by 4-hr and 7-day homologous passive cutaneous anaphylaxis (PCA) titer, respectively. In brief, serial doubling dilu tions of serum were made with saline from 250 to 16,000-fold.…”

Section: Chemicalsmentioning

confidence: 99%

Relationship between Density of Muscarinic Receptors and Nasal Hypersecretion in a Nasal Allergic Model

Namimatsu

Okazaki

et al. 1992

Japanese Journal of Pharmacology

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ABSTRACT-In guinea pigs actively sensitized with ovalbumin, we have observed that nasal hyper reactivity and hypersensitivity to methacholine causes nasal secretion accompanied by an increase in the density of muscarinic acetylcholine receptor (m-ACh•R) following the appearance of nasal symptoms induced by the ovalbumin challenge. Therefore in the present investigation, we studied the relationship between the density of m-ACh•R and nasal hypersecretion in actively sensitized guinea pigs. There was no relationship between the quantity of nasal secretion and serum Ig G1 or Ig E levels. On the other hand, the sensitivity to methacholine and nasal secretion induced by methacholine were significantly re lated to the density of m-ACh•R located on the nasal mucosa. The quantity of nasal secretion induced by allergen was also correlated significantly to the density of m-ACh•R. These results suggest that the nasal hypersecretion observed in the nasal allergic model may be closely associated with an increase in the density of m-ACh•R located on the nasal mucosa. Keywords:Muscarinic receptor, Nasal hypersensitivity, Allergic rhinitis, Nasal provocation, Serum antibody levelNasal allergy is generally a Type I allergic reaction taking place on the surface of basophils and mast cells which are located on the epithelium of the nasal cavity, and Ig E plays a key role in this reaction (1-3). In addition to its immunological mechanism, an imbalance of the autonomic nervous system has also been impli cated as one of the important factors in eliciting nasal allergic symptoms (4). In fact, an increase in density of muscarinic acetylcholine receptor (m-ACh•R) and a de crease in densities of al (a,-R) and /9-adrenergic recep tors (f3•R) were observed in the nasal mucosa of pa tients with nasal allergy (5-7). It is widely known that the nasal mucosa shows not only specific hyperreactivity to allergens but also non-specific hypersensitivity and hyperreactivity to neuromediators (8, 9), to irritants (10) and to physical stimuli (11). Thus, the changes in the expression of autonomic nerve receptors seem to be related to the appearance of hypersensitivity in the na sal mucosa. At present, however, there is little scien tific evidence about the relationship between the changes in the autonomic nerve receptors and the appearance of nasal mucosal hypersensitivity or of nasal allergy.In the present study, the relationship between the nasal hypersecretion and density of m-ACh•R was in vestigated by using guinea pigs actively sensitized with ovalbumin to clarify the influence of impaired function of the parasympathetic nervous system on the appear ance of nasal mucosal hypersensitivity. MATERIALS AND METHODS Experimental animalsMale Hartley guinea pigs (Japan SLC) weighing 400 to 500 g were used. The animals were housed in a temperature-controlled room at 24°C with a 12-hr light cycle (lights on from 08:00 to 20:00), and food and wa ter were given ad libitum. ChemicalsChemicals were purchased from the following com panies: Fluorescein sodium (ur...

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Section: Chemicalsmentioning

confidence: 99%

Relationship between Density of Muscarinic Receptors and Nasal Hypersecretion in a Nasal Allergic Model

Namimatsu

Okazaki

et al. 1992

Japanese Journal of Pharmacology

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“…These antibodies were shown to persist in the skin of guinea pigs for only a short time [5,9], More recently, three homologous guinea pig skin-sensitiz ing antibodies belonging to two separate im munoglobulin classes (IgG, and IgE) have been identified [10][11][12], These immuno globulins have been characterized by electro phoretic mobility, heat stability (56 C for 1 h), and the ability to persist in skin for varying lengths of time. The heat-stable IgG,., and IgG,,, persist in skin for 3-4 and for 7-9 days, respectively, and the heatlabile IgE persists in the skin for 21 days or more.…”

Section: Introductionmentioning

confidence: 99%

“…The heat-stable IgG,., and IgG,,, persist in skin for 3-4 and for 7-9 days, respectively, and the heatlabile IgE persists in the skin for 21 days or more. Immunization procedures have also been described for the guinea pig in which either of these immunoglobulin classes can be predominately prepared [4,8,10],…”

Section: Introductionmentioning

confidence: 99%

Systemic Persistence of Homologous Guinea Pig Skin-Sensitizing Antibodies

Megel¹,

Denney²

1979

Int Arch Allergy Immunol

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Homologous guinea pig skin-sensitizing antibodies of the IgG₁ class (as characterized by heat stability and persistence in skin for 7 days) were shown to persist systemically for 28–35 days. Persistence was shown by the anaphylaxis induced in guinea pigs following the intracardiac administration of the antibody and subsequent antigenic challenge by aerosol. Skin-sensitizing antibody of the IgE class, characterized by heat lability and persistence in skin for 14 days, still caused systemic anaphylaxis 42 days after the intracardiac administration of antibody. The IgG serum fraction from nonimmunized rabbits blocked systemic anaphylaxis in the guinea pig induced by aerosol following the passive transfer of heterologous (rabbit) and homologous IgG antibodies to ovalbumin, but not following the passive transfer of homologous IgE antibodies to ovalbumin.

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“…To exclude IgGl-mediated responses from antigen-induced bronchoconstriction in guineapigs sensitized with anti-BPO serum, we performed antigen challenge after a long latent period (14 days) following the i.v. administration of 5 fold diluted antiserum, based on the assumption that IgE antibody persists in the tissue for relatively longer periods than the IgGl antibody (Ovary et al, 1963;1976). This bronchoconstriction was almost completely reduced in guinea-pigs sensitized with 5 fold diluted anti-BPO serum that had been heated at 56TC for 4 h, indicating that there was no contamination of the IgGl-mediated response in this model.…”

Section: Ige-mediated Bronchoconstrictionmentioning

confidence: 88%

Contribution of thromboxane A₂ to the antigen‐induced immediate asthmatic response mediated by IgG1 antibody by augmentation of bronchial responsiveness in guinea‐pigs

Arimura

Asanuma

Kurosawa

et al. 1994

British J Pharmacology

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1 IgGI-mediated anaphylactic bronchoconstriction was elicited by intravenous administration of antigen to guinea-pig 2 days after passive sensitization with IgGl-rich serum, and this response was not affected by heating the serum (at 56°C, for 4 h). IgE-mediated bronchoconstriction, provoked 14 days after passive sensitization with IgE-rich serum, was completely abolished by the heating of the serum. 2 S-1452 (10 mg kg-', p.o.), a selective thromboxane (Tx) A2 antagonist, significantly but incompletely suppressed the IgGl-mediated bronchoconstriction, but did not affect the IgE-mediated one, while diphenhydramine (5 mg kg'-, i.v.), a histamine antagonist, almost completely inhibited both IgGI-and IgE-mediated bronchoconstriction.3 Pretreatment with propranolol (1 mg kg-', i.v.), a P-adrenergic blocker, in addition to diphenhydramine, caused a long-lasting bronchoconstriction following antigen challenge in both animal models. This histamine-independent bronchoconstriction was markedly suppressed by S-1452 at a low dose of 0.1 mg kg-'. 4 A significant increase in bronchial responsiveness to i.v. acetylcholine (ACh), compared to the prechallenge value, occurred as early as 3 min and persisted for 24 h after antigen challenge in the IgGI model, but was not observed in the IgE model. S-1452 (10mg kg-', p.o.) inhibited the IgGI-mediated bronchial hyperresponsiveness, as assessed 60 min after antigen challenge. 5 A marked elevation of TxB2 levels was observed in bronchoalveolar lavage fluid (BALF) 3 min after antigen challenge in the IgGl model, while levels were not changed in the IgE model. In contrast, the plasma TxB2 level assessed 1 min after antigen challenge was increased in both the IgGI and IgE models. 6 The results indicate that the inhibition of IgGl-but not IgE-mediated bronchoconstriction by higher doses of S-1452 may result from the suppression of increased bronchial responsiveness to allergic mediators such as histamine, which is probably due to TxA2 generated in the airway lumen rather than in plasma. In both the IgGI and IgE models, plasma TxA2 appeared to contribute directly to the bronchoconstriction, its action being almost completely masked by histamine-mediated bronchoconstriction.

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Characteristics of Guinea Pig IgE

Cited by 73 publications

References 15 publications

Relationship between Density of Muscarinic Receptors and Nasal Hypersecretion in a Nasal Allergic Model

Relationship between Density of Muscarinic Receptors and Nasal Hypersecretion in a Nasal Allergic Model

Systemic Persistence of Homologous Guinea Pig Skin-Sensitizing Antibodies

Contribution of thromboxane A₂ to the antigen‐induced immediate asthmatic response mediated by IgG1 antibody by augmentation of bronchial responsiveness in guinea‐pigs

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Characteristics of Guinea Pig IgE

Cited by 73 publications

References 15 publications

Relationship between Density of Muscarinic Receptors and Nasal Hypersecretion in a Nasal Allergic Model

Relationship between Density of Muscarinic Receptors and Nasal Hypersecretion in a Nasal Allergic Model

Systemic Persistence of Homologous Guinea Pig Skin-Sensitizing Antibodies

Contribution of thromboxane A2 to the antigen‐induced immediate asthmatic response mediated by IgG1 antibody by augmentation of bronchial responsiveness in guinea‐pigs

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Contribution of thromboxane A₂ to the antigen‐induced immediate asthmatic response mediated by IgG1 antibody by augmentation of bronchial responsiveness in guinea‐pigs