Characteristics of growth hormone response to the administration of growth hormone-releasing hormone (GRF) in the lamb

Barenton, Bruno; Duclos, M; Diaz, J.A. Cabranes; Deletang, F.; Dulor, J.-P.; Blanchard, Marie-Pierre; Charrier, J.; Garandel, Véronique; Fraysse, A.

doi:10.1051/rnd:19870405

Cited by 8 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After this, 125 mg of GHRH (1-29)-NH 2 were intravenously (iv) given, and three blood samples, 10, 20 and 30 min after the iv GHRH injection, were collected in EDTA-saline treated tubes to evaluate pituitary GH response. This schedule has been shown to cover all the relevant GH responsiveness (2). Blood was centrifuged and plasma stored at -20°C for subsequent GH and Insulin-like growth factor-I (IGF-I) assays.…”

Section: Methodsmentioning

confidence: 99%

“…Accordingly, numerous experiments have shown the efficacy of synthetic GHRH (1-29)-NH z in stimulating GH release in humans (4) and experimental animals such as rats (24), and lambs (2). In the last paper, exogenous sc intermittent administration of GHRH increased circulating GH concentrations (2), and the same peptide was found to raise G H levels when delivered by sc osmotic pumps (28). Therefore, this GHRH preparation seems to be appropriate for investigating dynamic changes of GH release and also to be used as a potential alternative to exogenous GH administration in the sheep.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Growth hormone response to long-term GH-RH administration in lambs

Perez-Romero

Rol

Granados

et al. 2000

J. Physiol. Biochem.

View full text Add to dashboard Cite

The pattern of long-term GHRH administration capable of stimulating GH release without depleting pituitary GH content has been investigated using two experimental approaches. In experiment 1, recently weaned male lambs were treated for 3 weeks as follows: Group A) control; B) subcutaneous (sc) continuous infusion of GHRH (1200 mg/day) using a slow release pellet; C) the same as B plus 1 daily sc injection of long acting somatostatin (SS) (octreotide, 20 mg) ; D) 3 daily sc GHRH (250 mg) injections ; E) 2 daily sc injections of GHRH (250 mg) and 2 of natural SS (250 mg). In experiment 2, recently weaned male lambs were continuously GHRH-treated using sc osmotic minipumps (900 mg/day) alone or combined with a daily sc injection of octreotide (20 mg) for 4 weeks. Basal plasma GH levels were increased after chronic pulsatile GHRH treatment but not after any kind of continuous GHRH administration. This increment was maintained during the 3 weeks of experimentation and appeared accompanied by a pituitary GH content similar to controls. A marked GH response to the iv GHRH challenge was observed in controls and in lambs receiving both types of continuous sc GHRH infusions, whereas pulsatile sc GHRH-treated animals did not respond to the iv GHRH challenge in the first and second weeks of the study but did so in the third week of treatment. These data demonstrate that long-term pulsatile GHRH administration is capable of stimulating GH release in growing male lambs, without producing pituitary desensitization.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Growth hormone response to long-term GH-RH administration in lambs

Perez-Romero

Rol

Granados

et al. 2000

J. Physiol. Biochem.

View full text Add to dashboard Cite

show abstract

The Growth Hormone Secretory Pattern and Statural Growth

Robinson¹,

Hindmarsh

1999

Comprehensive Physiology

View full text Add to dashboard Cite

The sections in this article are: How is the Growth Hormone Secretory Pattern Analyzed? General Considerations in Pulse Analysis of Growth Hormone Secretion Construction of Data Arrays or Hormone Profiles Sampling Interval and Aliasing Duration of Sampling Discrete or Integrated Samples Assays and Noise Basic Evaluation Stationarization Analysis of Profiles Time Series Analysis Deconvolution Analysis Baseline Occupancy Chaos Neural Networks Characterization of Growth Hormone Secretory Patterns Ontogeny of Episodic Growth Hormone Secretion Growth Hormone Secretory Patterns in the Prepubertal Period Puberty and Effects of Gonadal Steroids on the Growth Hormone Secretory Pattern Growth Hormone Secretory Patterns in Adulthood Other Endocrine Inputs Affecting Growth Hormone Secretory Patterns Extrinsic Factors Affecting Growth Hormone Pulsatility How Does the Growth Hormone Secretory Pattern Reflect the Episodic Nature of Hypothalamic Control Mechanisms? Growth Hormone–Releasing Hormone and Somatostatin Interactions Generate Growth Hormone Pulsatility Other Growth Hormone Secretogogues Growth Hormone Feedback Effects on Endogenous Growth Hormone Pulsatility How is The Growth Hormone Secretory Signal Modified in its Passage from the Pituitary to the Peripheral Target Tissues? How Are Growth Hormone Patterns Modified by Interactions with Growth Hormone Binding Proteins? How are the Temporal Elements of the Growth Hormone Signal Detected and Interpreted by the Target Tissues? Pattern‐Dependent Growth Hormone Responses Growth Hormone Patterns and Insulin‐Like Growth Factor I Generation What is the Significance of the Growth Hormone Secretory Pattern for Statural Growth? Animal Studies Human Studies Indirect Manipulation of Growth Hormone Secretory Pattern Summary

show abstract