Characteristics of Group B Streptococcus Isolated from Infants with Invasive Infections: A Population-Based Study in Japan

Chang, Bin; Wada, Akihito; Oishi, Tomohiro; Ishiwada, Naruhiko; Oda, Megumi; Sato, Tetsuya; Terauchi, Yoshihiko; Okada, Kenji; Nishi, Junichiro; Akeda, Hideki; Kamiya, Hitoshi; Ohnishi, Makoto; Ihara, Toshiaki

doi:10.7883/yoken.67.356

Cited by 22 publications

(21 citation statements)

References 23 publications

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“…The overall incidence of invasive neonatal GBS disease (0.55 per 1000 live births, 95% CI 0.44–0.69) in Guangdong between 2011 and 2014, was close to the global average estimation (0.53 per 1000 live births, 95% CI 0.44–0.62) [ 5 ]. However the incidence rate in this study is much higher than which was suggested in our previous short-term observation [ 10 ] and in other Asian countries with the incidences rate 0.14–0.41 per 1000 live births [ 12 , 13 ]. Furthermore, GBS in EOD increased markedly from 2011 to 2014, reaching 0.58 per 1000 live births.…”

Section: Discussioncontrasting

confidence: 71%

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Epidemiology of invasive group B streptococcal disease in infants from urban area of South China, 2011–2014

Guan

et al. 2018

BMC Infect Dis

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BackgroundGroup B Streptococcus (GBS) is a leading cause of morbidity and mortality in infants in both developed and developing countries. To our knowledge, only a few studies have been reported the clinical features, treatment and outcomes of the GBS disease in China. The severity of neonatal GBS disease in China remains unclear. Population-based surveillance in China is therefore required.MethodsWe retrospectively collected data of <3 months old infants with culture-positive GBS in sterile samples from three large urban tertiary hospitals in South China from Jan 2011 to Dec 2014. The GBS isolates and their antibiotic susceptibility were routinely identified in clinical laboratories in participating hospitals. Serotyping and multi-locus sequence typing (MLST) were also conducted for further analysis of the neonatal GBS disease.ResultsTotal 70 cases of culture-confirmed invasive GBS infection were identified from 127,206 live births born in studying hospitals, giving an overall incidence of 0.55 per 1000 live births (95% confidence interval [CI] 0.44–0.69). They consisted of 49 with early-onset disease (EOD, 0.39 per 1000 live births (95% CI 0.29–0.51)) and 21 with late-onset disease (LOD, 0.17 per 1000 live births (95% CI 0.11–0.25)). The incidence of EOD increased significantly over the studying period. Five infants (4 EOD and 1 LOD) died before discharge giving a mortality rate of 7.1% and five infants (7.1%, 2 EOD and 3 LOD) had neurological sequelae. Within 68 GBS isolates from GBS cases who born in the studying hospitals or elsewhere, serotype III accounted for 77.9%, followed by Ib (14.7%), V (4.4%), and Ia (2.9%). MLST analysis revealed the presence of 13 different sequence types among the 68 GBS isolates and ST-17 was the most frequent sequence type (63.2%). All isolates were susceptible to penicillin, ceftriaxone, vancomycin and linezolid, while 57.4% and 51.5% were resistant to erythromycin and clindamycin, respectively.ConclusionsThis study gains the insight into the spectrum of GBS infection in south China which will facilitate the development of the guidance for reasonable antibiotics usage and will provide evidence for the implementation of potential GBS vaccines in the future.Electronic supplementary materialThe online version of this article (10.1186/s12879-017-2811-0) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 71%

“…In this study the proportion of EOD (70%) is higher than that of LOD (30%). In other Asian countries such as Japan and Korea, reported that the LOD account for 75% (45/60) and 80% (125/157), respectively [ 13 , 15 ]. An important risk factor for EOD is GBS colonization of maternal vaginal and rectal sites during late pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of invasive group B streptococcal disease in infants from urban area of South China, 2011–2014

Guan

et al. 2018

BMC Infect Dis

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“…We calculated a pooled 39% (95%CI:25–53) of maternal colonisation isolates as serotypes VI-IX (from 5 studies, n = 728 isolates) [62] , [71] , [72] , [73] , [74] . However, we found these serotypes to be less frequently reported as causing invasive disease in infants, with 8% (95%CI:2–15) of EOGBS (4studies) [75] , [76] , [77] , [78] , and 1% (95%CI:0–3) of LOGBS (3studies) [75] , [77] , [78] , while serotype III still predominates in these cases with 38% (95%CI:26–51) in EOGBS and 36% (95%CI:3–79) in LOGBS. Protein vaccines could theoretically reduce the risk of serotype replacement that theoretically exists for GBS capsular-polysaccharide based vaccines.…”

Section: Discussionmentioning

confidence: 71%

Systematic review of Group B Streptococcal capsular types, sequence types and surface proteins as potential vaccine candidates

Bianchi-Jassir

Paul

et al. 2020

Vaccine

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“…The high specificity of Real-LAMP relied on well-designed primers, which ensured strict alignment between primers and targeted region, not only facilitating major amplification of the targeted sequence but also differentiating one-base pair-difference among all similar templates; to avoid any interference with non-targeted DNA sequences [6]. In the present study, LAMP was performed to detect the presence of DNA samples for Sa and 10 other strains of common pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Streptococcus agalactiae

et al. 2019

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Streptococcus agalactiae is a major pathogenic bacterium causing perinatal infections in humans. In the present study, a novel real-time fluorescence loop-mediated isothermal amplification technology was successfully developed and evaluated for the detection of S. agalactiae in a single reaction. Six specific primers were designed to amplify the corresponding six regions of fbs B gene of S. agalactiae, using Bst DNA polymerase with DNA strand displacement activity at a constant temperature for 60 min. The presence of S. agalactiae was indicated by the fluorescence in real-time. Amplification of the targeted gene fragment was optimized with the primer 1 in the current setup. Positive result was only obtained for Sa by Real-LAMP among 10 tested relevant bacterial strains, with the detection sensitivity of 300 pg/µl. Real-LAMP was demonstrated to be a simple and rapid detection tool for S. agalactiae with high specificity and stability, which ensures its wide application and broad prospective utilization in clinical practice for the rapid detection of S. agalactiae.

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Characteristics of Group B Streptococcus Isolated from Infants with Invasive Infections: A Population-Based Study in Japan

Cited by 22 publications

References 23 publications

Epidemiology of invasive group B streptococcal disease in infants from urban area of South China, 2011–2014

Epidemiology of invasive group B streptococcal disease in infants from urban area of South China, 2011–2014

Systematic review of Group B Streptococcal capsular types, sequence types and surface proteins as potential vaccine candidates

Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Streptococcus agalactiae

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