Characteristics of fermentative hydrogen production with Klebsiella pneumoniae ECU-15 isolated from anaerobic sewage sludge

Niu, Kun; Zhang, Xu; Tan, Wen‐Song; Zhu, Mingming

doi:10.1016/j.ijhydene.2009.10.071

Cited by 80 publications

(38 citation statements)

References 45 publications

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“…Preparation of the bioPd(0) catalysts K. pneumoniae ECU-15 was isolated from the anaerobic sewage sludge [12] and cultured to a high-cell concentration in an experimental reactor. The biomass at midlogarithmic phase cultures was harvested by a centrifuge (4000 g, 15 min, 4°C).…”

Section: Methodsmentioning

confidence: 99%

“…An addition of Pd(II) to the fermenting culture of Clostridium pasteurianum could result in the formation of Pd(0) nanoparticles on the bacterial cell wall and in the cytoplasm [11]. Klebsiella pneumoniae is a potential biohydrogen producer [12] and has the ability to bioreduce Pd(II) [13]. The evaluation of the capacity of K. pneumoniae to reduce Pd(II) to Pd(0) nanoparticles would be significant because of its respiration diversity, its wide distribution in various environments, and its potentially effective approach to the remediation of aggressive metal waste.…”

Section: Introductionmentioning

confidence: 99%

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Effect of fluid shear stress on catalytic activity of biopalladium nanoparticles produced by Klebsiella Pneumoniae ECU-15 on Cr(VI) reduction reaction

Lei

Zhang

Zhu

et al. 2014

Bioresour. Bioprocess.

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Background: Biopalladium (bioPd(0)) nanoparticles on Klebsiella Pneumoniae ECU-15 were synthesized mainly on the microorganism's surface. Data suggest that the resistance of mass transfer around the cell surface region plays a critical role in bioPd(0) synthesis process. However, the mechanisms for its role remains elusive. Results: The experimental results indicated that 1) diffusion resistance existed around the microorganism's cell in reaction vessel and 2) fluid shear stress affected the mass transfer rates differently according to its strength and thus had varying effects on the bioPd(0) synthesis. More than 97.9 ± 1.5% Chromium(VI)(Cr(VI)) (384 μM) was reduced to Cr(III) within 20 min with 5% Pd/bioPd(0) as catalyst, which was generated by the K. Pneumoniae ECU-15, and the catalytic performance of Pd/bioPd(0) was stable over 6 months. The optimal condition of bioreduction of Pd(II) to Pd(0) was determined at the Kolmogorov eddy length of 7.33 ± 0.5 μm and lasted for 1 h in the extended reduction process after the usual adsorption and reduction process.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of fluid shear stress on catalytic activity of biopalladium nanoparticles produced by Klebsiella Pneumoniae ECU-15 on Cr(VI) reduction reaction

Lei

Zhang

Zhu

et al. 2014

Bioresour. Bioprocess.

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“…Anaerobic granular sludge, which is a rich source of H 2 -producing bacteria due to its high specific metabolic activity and microbial diversity, has been used as inoculum in numerous biohydrogen studies [2,10,11]. Concerning the substrate, several lowcost, common feedstocks such as molasses, lignocellulose hydrolysate, biodiesel and other industrial wastes, food wastes, and organic municipal wastes have been studied in previous works related to H 2 production [12,13].…”

Section: Introductionmentioning

confidence: 99%

Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

Rajhi

Puyol

Martínez³

et al. 2015

Front. Environ. Sci. Eng.

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The successful operation of any type of hydrogen-producing bioreactor depends on the performance of the microorganisms present in the system. Both substrate and partial gas pressures are crucial factors affecting dark fermentation metabolic pathways. The main objective of this study was to evaluate the impact of both factors on hydrogen production using anaerobic granular sludge as inoculum and, secondly, to study the metabolic shifts of an anaerobic community subjected to low partial gas pressures. With this goal in mind, seven different wastewater (four synthetic media, two industrial wastewater, and one domestic effluent) and the effect of applying vacuum on the systems were analyzed. The application of vacuum promoted an increase in the diversity of hydrogenproducing bacteria, such as Clostridium, and promoted the dominance of acetoclastic-over hydrogenotrophic methanogens. The application of different media promoted a wide variety of metabolic pathways. Nevertheless, reduction of the hydrogen partial pressure by application of vacuum lead to further oxidation of reaction intermediates irrespective of the medium used, which resulted in higher hydrogen and methane production, and improved the COD removal. Interestingly, vacuum greatly promoted biogenic hydrogen production from a real wastewater, which opens possibilities for future application of dark fermentation systems to enhance biohydrogen yields.

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“…Hydrogen is an energetically promising alternative, mainly because it is an energetic vector that allows electricity generation with high efficiency, has high calorific value per weight unit, and its combustion in the presence of oxygen emits water vapor only (Lattin et al, 2007;Niu et al 2010). There is a high variety of hydrogen-producing technologies that require the use of external non-renewable energy, representing a strong economical and environmental disadvantage (Elam et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Adaptation of acidogenic sludge to increasing glycerol concentrations for biohydrogen production

Tapia-Venegas

Cabrol

Brandhoff

et al. 2015

Appl Microbiol Biotechnol

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Hydrogen is a promising alternative as an energetic carrier and its production by dark fermentation from wastewater has been recently proposed, with special attention to crude glycerol as potential substrate. In this study, two different feeding strategies were evaluated for replacing the glucose substrate by glycerol substrate: a one-step strategy (glucose was replaced abruptly by glycerol) and a step-by-step strategy (progressive decrease of glucose concentration and increase of glycerol concentration from 0 to 5 g L(-1)), in a continuous stirred tank reactor (12 h of hydraulic retention time (HRT), pH 5.5, 35 °C). While the one-step strategy led to biomass washout and unsuccessful H2 production, the step-by-step strategy was efficient for biomass adaptation, reaching acceptable hydrogen yields (0.4 ± 0.1 molH2 mol(-1) glycerol consumed) around 33 % of the theoretical yield independently of the glycerol concentration. Microbial community structure was investigated by single-strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) fingerprinting techniques, targeting either the total community (16S ribosomal RNA (rRNA) gene) or the functional Clostridium population involved in H2 production (hydA gene), as well as by 454 pyrosequencing of the total community. Multivariate analysis of fingerprinting and pyrosequencing results revealed the influence of the feeding strategy on the bacterial community structure and suggested the progressive structural adaptation of the community to increasing glycerol concentrations, through the emergence and selection of specific species, highly correlated to environmental parameters. Particularly, this work highlighted an interesting shift of dominant community members (putatively responsible of hydrogen production in the continuous stirred tank reactor (CSTR)) according to the gradient of glycerol proportion in the feed, from the family Veillonellaceae to the genera Prevotella and Clostridium sp., putatively responsible of hydrogen production in the CSTR.

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Characteristics of fermentative hydrogen production with Klebsiella pneumoniae ECU-15 isolated from anaerobic sewage sludge

Cited by 80 publications

References 45 publications

Effect of fluid shear stress on catalytic activity of biopalladium nanoparticles produced by Klebsiella Pneumoniae ECU-15 on Cr(VI) reduction reaction

Effect of fluid shear stress on catalytic activity of biopalladium nanoparticles produced by Klebsiella Pneumoniae ECU-15 on Cr(VI) reduction reaction

Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

Adaptation of acidogenic sludge to increasing glycerol concentrations for biohydrogen production

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