Characteristics of Extracellular Protein Production by Staphylococcus simulans biovar staphylolyticus during Aerobic and Anaerobic Growth

Donham, Michael C.; Heath, Harry E.; LeBlanc, Paul A.; Sloan, Gary L.

doi:10.1099/00221287-134-9-2615

Cited by 9 publications

(18 citation statements)

References 17 publications

(5 reference statements)

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“…Some coagulase-negative staphylococci have been shown to produce extracellular proteins (Donham et al, 1988) and slime (Bayston & Rodgers, 1990), mostly during the post-exponential growth phase. It is tempting to speculate that these species possess a common regulatory mechanism exhibiting some homologies with S. aureus agr.…”

Section: Discussionmentioning

confidence: 99%

Involvement of the accessory gene regulator (agr) in expression of type 5 capsular polysaccharide by Staphylococcus aureus

Dassy

Hogan²,

Foster³

et al. 1993

Journal of General Microbiology

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The effect of an agr mutation on expression of type 5 capsular polysaccharide (CP) by Staphylococcus aureus Newman was investigated in different complex and synthetic media. CP expression by the agr mutant was strongly reduced in certain media but slightly in others, indicating that CP synthesis is positively controlled by agr. CP expression occurred in the post-exponential growth phase in both wild-type and mutant strains, suggesting that other regulatory systems could act in conjunction with agr.

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Section: Discussionmentioning

confidence: 99%

Involvement of the accessory gene regulator (agr) in expression of type 5 capsular polysaccharide by Staphylococcus aureus

Dassy

Hogan²,

Foster³

et al. 1993

Journal of General Microbiology

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“…simulans biovar staphylolyticus secretes pro-endopeptidase (M r 59 K), which is proteolytically processed to endopeptidase (M r 26 K) [5,14,15]. In our studies of extracellular protein production by this organism, we have detected only one proteolytic activity, which is due to sulphydryl protease [7][8][9][10]13].…”

Section: Introductionmentioning

confidence: 95%

“…Samples were electrophoresed as described above and transblotted to nitrocellulose as previously described [5]. Blotted membranes were exposed to either endopeptidase-specific monoclonal antibody ALS8.1 [5] or rabbit antisera prepared against purified hexosaminidase [20].…”

Section: Western Blot Analysismentioning

confidence: 99%

“…The second is a micrococcolytic endo-/3-N-acetylglucosaminidase (hexosaminidase) that hydrolyzes the bond between N-acetylglucosamine and N-acetylmuramic acid [3,4]. Endopeptidase, hexosaminidase and extra- cellular sulphydryl protease activities are produced coordinately under a variety of conditions [5][6][7][8][9][10]. However, the corresponding genes are not in the same unit of transcription because the endopeptidase gene resides on a plasmid whereas the genes for hexosaminidase and protease are on the chromosome [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Extracellular proteolytic activation of bacteriolytic peptidoglycan hydrolases ofStaphylococcus simulansbiovarstaphylolyticus

Neumann

Heath

LeBlanc

et al. 1993

FEMS Microbiology Letters

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Staphylococcus simulans biovar staphylolyticus secreted two bacteriolytic peptidoglycan hydrolases as proproteins that were activated as they were processed by an extracellular sulphydryl protease. This processing resulted in the production of multiple molecular-mass forms of each enzyme. Cells from early exponential phase cultures were susceptible to lysis by the mature forms of each of the peptidoglycan hydrolases whereas stationary phase cells were resistant. Thus secretion of these bacteriolytic enzymes during early exponential growth as precursors that are activated later by the protease would provide time for the cells to become resistant.

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“…Culture supernatants were tested for reactivity with lysostaphin-specific MAb ALS8.1 (18) by an enzymelinked immunosorbent assay and by immunoblotting. For the enzyme-linked immunosorbent assay, supernatants were mixed with an equal volume of 45 mM sodium carbonate-bicarbonate buffer (pH 9.6) and added to 96-well plates for 2 h at 37°C before blocking with bovine serum albumin (3 mg/ml) and subsequent exposure to ALS8.1.…”

Section: (41)mentioning

confidence: 99%

Ribotype Delineation and Description of Staphylococcus sciuri Subspecies and Their Potential as Reservoirs of Methicillin Resistance and Staphylolytic Enzyme Genes

Kloos

Ballard

Webster

et al. 1997

International Journal of Systematic Bacteriology

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Three subspecies of Staphylococcus sciuri, S. sciuri subsp. sciuri Moos, Schleifer, and Smith 1976, 23& emend. Moos et al. 1996, S. sciuri subsp. curnaticus subsp. nov., and S. sciuri subsp. rodentium subsp. nov., are described on the basis of their ribotype patterns, DNA-DNA liquid hybridization data, and phenotypic characteristics. Normalized ribotyping subdivided the S. sciuri patterns into three blocks of patterns, each corresponding to a subspecies. Each subspecies formed a separate, well-defined DNA similarity group when DNA-DNA hybridizations were conducted under stringent (7OOC) reassociation conditions. S. sciuri subsp. sciuri could be distinguished from the other subspecies on the basis of its ability to produce acid from D-cellobiose, alkaline phosphatase activity, and inability to produce either clumping factor or protein A. S. sciuri subsp. curnaticus could be distinguished by its ability to produce acid aerobically from D-xylose and maltose, inability to produce acid from D-melezitose, and smaller colony size on P agar. S. sciuri subsp. rodentium could be distinguished by its positive reaction in the latex agglutination test for clumping factor and/or protein A and generally higher frequencies and levels of oxacillin and methicillin resistance. All 40 strains of S. sciuri tested (including representatives of all three subspecies) hybridized with the mecA gene probe. All strains of S. sciuri subsp. sciuri, 79% of the strains of S. sciuri subsp. carnuticus and 89% of the strains of S. sciuri subsp. rodentium exhibited extracellular, staphylolytic enzyme activity. This activity was associated with an enzyme(s) that immunoblotted with a lysostaphin-specific monoclonal antibody; however, only three strains hybridized with a lysostaphin (end) gene probe. The type strain of S. sciuri subsp. curnaticus is DD 791 (= ATCC 700058), and the type strain of S. sciuri subsp. rodentium is DD 4761 (= ATCC 700061).Staphylococcus sciuri is generally considered one of the most primitive Staphylococcus species; it is widely distributed in nature, is capable of growth on inorganic nitrogen salts as the sole source of nitrogen, and exhibits a wide range of biochemical activities (31-33). In the past 20 years since its original description (33), numerous laboratories have reported frequent isolation of this species from foods, farm animals, rodents, marsupials, marine mammals, and birds and occasional isolation from humans and their pets (1,2, 17,28,31,35,48,52,57,58).Most resident populations of S. sciuri are members of the normal cutaneous microflora of lower mammals. This species is rarely associated with infections. A preliminary investigation of a large collection of S. sciuri strains showed that a ribotyping method based on an analysis of genomic EcoRI fragments containing portions of the rRNA operons could be used to distinguish two major subgroups and additional strain variation within the species (10).In this study, we describe three subpopulations of S. sciuri which can be distinguished on the basis of their rib...

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Characteristics of Extracellular Protein Production by Staphylococcus simulans biovar staphylolyticus during Aerobic and Anaerobic Growth

Cited by 9 publications

References 17 publications

Involvement of the accessory gene regulator (agr) in expression of type 5 capsular polysaccharide by Staphylococcus aureus

Involvement of the accessory gene regulator (agr) in expression of type 5 capsular polysaccharide by Staphylococcus aureus

Extracellular proteolytic activation of bacteriolytic peptidoglycan hydrolases ofStaphylococcus simulansbiovarstaphylolyticus

Ribotype Delineation and Description of Staphylococcus sciuri Subspecies and Their Potential as Reservoirs of Methicillin Resistance and Staphylolytic Enzyme Genes

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