Characteristics of Extended-Spectrum β-Lactamases-Producing &lt;i&gt;Escherichia coli&lt;/i&gt; in Fecal Samples of Inpatients of Beijing Tongren Hospital

Xu, Mingqing; Wang, Mei; Lu, Xinxin

doi:10.7883/yoken.jjid.2016.023

Cited by 7 publications

(4 citation statements)

References 26 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ESBL-PE carriage was significantly higher in hospitalized patients compared with healthy volunteers (51% vs. 38%, p < 0.05), as previously described in similar settings [25–28]. This result could be explained by higher antibiotic consumption that favors ARB selection and/or higher rate of in-hospital acquisition of ARB that then colonize the patient’s intestinal track [29–31]. Several factors can contribute to high ARB selection in low income countries, particularly poor drug quality or inadequate posology, long treatments, antibiotic misuse by health professionals, unskilled practitioners, auto-medication (antibiotics can be purchased without prescription), unhygienic conditions accounting for the spread of resistant bacteria, and inadequate surveillance programs [9, 32, 33].…”

Section: Discussionsupporting

confidence: 65%

Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad

Mahamat

Tidjani

Lounnas

et al. 2019

Antimicrob Resist Infect Control

View full text Add to dashboard Cite

BackgroundFecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) remains poorly documented in Africa. The objective of this study was to determine the prevalence of ESBL-PE fecal carriage in Chad.MethodsIn total, 200 fresh stool samples were collected from 100 healthy community volunteers and 100 hospitalized patients from January to March 2017. After screening using ESBL-selective agar plates and species identification by MALDI-TOF mass spectrometry, antibiotic susceptibility was tested using the disk diffusion method, and ESBL production confirmed with the double-disc synergy test. The different ESBL genes in potential ESBL-producing isolates were detected by PCR and double stranded DNA sequencing. Escherichia coli phylogenetic groups were determined using a PCR-based method.ResultsESBL-PE fecal carriage prevalence was 44.5% (51% among hospitalized patients vs 38% among healthy volunteers; p < 0.05). ESBL-producing isolates were mostly Escherichia coli (64/89) and Klebsiella pneumoniae (16/89). PCR and sequencing showed that 98.8% (87/89) of ESBL-PE harbored blaCTX-M genes: blaCTX-M-15 in 94.25% (82/87) and blaCTX-M-14 in 5.75% (5/87). Phylogroup determination by quadruplex PCR indicated that ESBL-producing E. coli isolates belonged to group A (n = 17; 27%), C (n = 17; 27%), B2 (n = 9; 14%), B1 (n = 8; 13%), D (n = 8; 13%), E (n = 1; 1.6%), and F (n = 1; 1.6%). The ST131 clone was identified in 100% (9/9) of E. coli B2 strains.ConclusionsThe high fecal carriage rate of ESBL-PE associated with CTX-M-15 in hospital and community settings of Chad highlights the risk for resistance transmission between non-pathogenic and pathogenic bacteria.

show abstract

Section: Discussionsupporting

confidence: 65%

Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad

Mahamat

Tidjani

Lounnas

et al. 2019

Antimicrob Resist Infect Control

View full text Add to dashboard Cite

show abstract

“…Desta et al, (17) in Ethiopia and Kurz (15) in Rwanda also reported high colonisation rates of 52% and 50% respectively in their studies. The findings of our study and those of others could be explained by high antibiotic consumption that favors selection of antibiotic resistant bacteria (ARB) and/or high rate of inhospital acquisition of ARB that then colonize the patient's intestinal tract (26,27). Several factors can contribute to high ARB selection in low-income countries, particularly from poor drug quality or inadequate posology, long treatment, increase antimicrobial use by healthcare professionals, unskilled practitioners, auto-medication (because antibiotics can be purchased without prescription), poor hygiene, resulting in the spread of resistant bacteria, and inadequate surveillance programs (28)(29)(30).…”

Section: Discussionsupporting

confidence: 54%

High faecal carriage of extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) among hospitalized patients at Sylvanus Olympio Teaching Hospital, Lomé, Togo in 2019

Godonou¹,

Lack²,

Gbeasor-Komlanvi³

et al. 2022

Af J Clin Exp Micro

View full text Add to dashboard Cite

Background: Extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) are a global health concern, associated with increased morbidity and mortality. Even in the absence of infections, colonization by these pathogens is still a great threat because of the risk of cross transfer among hospitalized patients. Faecal carriage of ESBL-PE remained poorly documented in Africa. This study aimed to determine faecal carriage rate of ESBL-PE, factors associated with carriage, and antimicrobial susceptibility of the strains among hospitalized patients at Sylvanus Olympio Teaching Hospitals (CHU SO) in Lomé, Togo. Methodology: This was a cross-sectional study of 105 randomly selected hospitalized patients between September and November 2019. Socio-demographic and clinical data as well as rectal swabs were collected after obtaining the consent of the selected participants. Rectal swabs were cultured on selective bromocresol purple (BCP) lactose agar containing 6µg/l ceftazidime, for isolation of Enterobacteriaceae. Identification of each isolate was performed using Uriselect 4 medium and API 20E. Antibiotic susceptibility of the bacterial isolates was performed by the Bauer-Kirby agar disc diffusion test and interpreted according to CASFM-EUCAST recommendations. Results: The faecal carriage rate of ESBL-PE among selected hospitalized patients was 80.9% (85/105). Escherichia coli was the most frequent bacteria 69.5% (73/105), followed by Klebsiella pneumoniae 22.8% (24/105). The antibiotic profile of ESBL producing Escherichia coli showed resistance to amoxycillin+clavulanic acid (72.6%), ticarcillin+clavulanic acid (82.2%), piperacillin+tazobactam (30.1%), cefoxitin (30.1%) ciprofloxacin (84.9%), levofloxacin (76.7%), nalidixic acid (83.6%), chloramphenicol (26.0%), gentamicin (49.3%), sulfamethoxazole-trimethoprim (86.3%), imipenem (5.5%), and ertapenem (21.9%). All (100%) isolates were sensitive to amikacin and fosfomycin. None of the characteristics or risk factors assessed was significantly associated with faecal carriage of ESBL-PE. Conclusion: Faecal carriage rate of ESBL-PE in these hospitalized patients was very high, but no factor was associated with carriage of ESBL-PE among the study population. Implementation of infection control measures, and surveillance are needed to limit the spread of these resistant pathogens within CHU SO healthcare facilities.

show abstract

“…the rate was reported to be 14.7% in Bavarian nursing home residents and 6.3% in the healthy population 11 . The prevalence of ESBL producing E. coli in fecal samples of 650 inpatients reported in a study in China, Beijing, was 25.7% 14 , while a study from Ankara, Turkey, reported the ESBL carriage rate at 34.3% for 1402 outpatients with E. coli strains being the most frequent ones 15 .…”

Section: Discussionmentioning

confidence: 94%

Extended-spectrum beta-lactamases found in Escherichia coli isolates obtained from blood cultures and corresponding stool specimen

Doerr,

Dietze,

Lippmann

et al. 2023

Sci Rep

View full text Add to dashboard Cite

With extended-spectrum β-lactamases (ESBLs) and CTX-M enzymes being on the rise, antimicrobial treatment of enterobacterial infections is becoming more and more challenging. Our study aimed at a molecular characterization of phenotypically ESBL-positive E. coli strains obtained from blood cultures of patients of the University Hospital of Leipzig (UKL), Germany. The presence of CMY-2, CTX-M-14 and CTX-M-15 was investigated using Streck ARM-D Kit (Streck, USA). Real-time amplifications were performed by QIAGEN Rotor-Gene Q MDx Thermocycler (QIAGEN, Thermo Fisher Scientific, USA). Antibiograms as well as epidemiological data were evaluated. Among 117 cases, 74.4% of the isolates showed a resistance to ciprofloxacin, piperacillin and ceftazidime or cefotaxime while being susceptible to imipenem/meropenem. The proportion of ciprofloxacin resistance was significantly higher than the proportion of ciprofloxacin susceptibility. At least one of the investigated genes was detected in 93.1% of the blood culture E. coli isolates: CTX-M-15 (66.7%), CTX-M-14 (25.6%) or the plasmid-mediated ampC gene CMY-2 (3.4%). 2.6% were tested positive for two resistance genes. 94 of the corresponding stool specimens tested positive for ESBL producing E. coli (94/112, 83.9%). 79 (79/94, 84%) E. coli strains found in the stool samples matched with the respective patient’s blood culture isolate phenotypically (MALDI-TOF, antibiogram). The distribution of resistance genes was in accordance with recent studies in Germany as well as worldwide. This study provides indications of an endogenous focus of infection and emphasize the importance of screening programs for high-risk patients.

show abstract

Characteristics of Extended-Spectrum β-Lactamases-Producing <i>Escherichia coli</i> in Fecal Samples of Inpatients of Beijing Tongren Hospital

Cited by 7 publications

References 26 publications

Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad

Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad

High faecal carriage of extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-PE) among hospitalized patients at Sylvanus Olympio Teaching Hospital, Lomé, Togo in 2019

Extended-spectrum beta-lactamases found in Escherichia coli isolates obtained from blood cultures and corresponding stool specimen

Contact Info

Product

Resources

About