Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic

Skočková, Alena; Karpíšková, Renata; Koláčková, Ivana; Cupáková, Šárka

doi:10.1016/j.ijfoodmicro.2013.09.011

Cited by 28 publications

(13 citation statements)

References 23 publications

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“…Tetracycline resistance was encoded by tetA and tetB genes in 11 and one tetracycline-resistant isolates, respectively. This finding was also reported by other studies (Koo and Woo, 2011 ; Xibiao et al, 2011 ), while the tetC gene is rarely reported (Skoèková et al, 2013 ). The sul1 , sul2 and sul3 genes were detected in 3, 17, and 5 isolates, respectively, not only amongst sulfonamid resistant isolates, but also in the six-sulfonamide susceptible isolates.…”

Section: Discussionsupporting

confidence: 86%

Occurrence of blaCTX-M-1,qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia

Kilani

Abbassi

Ferjani

et al. 2015

Front. Cell. Infect. Microbiol.

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Avian ESBL-producing Escherichia coli isolates have been increasingly reported worldwide. Animal to human dissemination, via food chain or direct contact, of these resistant bacteria has been reported. In Tunisia, little is known about avian ESBL- producing E. coli and further studies are needed. Seventeen ESBL-producing Escherichia coli isolates from poultry feces from two farms (Farm 1 and farm 2) in the North of Tunisia have been used in this study. Eleven of these isolates (from farm 1) have the same resistance profile to nalidixic acid, sulfonamides, streptomycin, tetracycline, and norfloxacine (intermediately resistant). Out of the six isolates recovered from farm 2, only one was co-resistant to tetracycline. All isolates, except one, harbored blaCTX-M-1 gene, and one strain co-harbored the blaTEM-1 gene. The genes tetA and tetB were carried, respectively, by 11 and 1 amongst the 12 tetracycline-resistant isolates. Sulfonamides resistance was encoded by sul1, sul2, and sul3 genes in 3, 17, and 5 isolates, respectively. The qnrB1 was detected in nine strains, one of which co-harbored qnrS1 gene. The search for the class 1 and 2 integrons by PCR showed that in farm 1, class 1 and 2 integrons were found in one and ten isolates, respectively. In farm 2, class 1 integron was found in only one isolate, class 2 was not detected. Only one gene cassette arrangement was demonstrated in the variable regions (VR) of the 10 int2-positive isolates: dfrA1- sat2-aadA1. The size of the VR of the class 1 integron was approximately 250 bp in one int1-positive isolate, whereas in the second isolate, no amplification was observed. All isolates of farm 1 belong to the phylogroup A (sub-group A0). However, different types of phylogroups in farm 2 were detected. Each of the phylogroups A1, B22, B23 was detected in one strain, while the D2 phylogroup was found in 3 isolates. The virulence genes iutA, fimH, and traT were detected in 3, 7, and 3 isolates, respectively. Two types of gene combination were detected: iutA+fimH+traT in 3 isolates and iutA+fimH in one isolate. The isolates recovered in farm 1 showed the same profile of PFGE macro-restriction, while isolates of farm 2 presented unrelated PFGE patterns. We conclude that these avian ESBL-producing E. coli isolates show homo- and heterogenic genetic background and that plasmids harboring ESBL genes could be involved in the dissemination of this resistance phenotype.

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Section: Discussionsupporting

confidence: 86%

Occurrence of blaCTX-M-1,qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia

Kilani

Abbassi

Ferjani

et al. 2015

Front. Cell. Infect. Microbiol.

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“…11,12 In recent years, the contamination of vegetables by E. coli O157:H7 has increased and is regarded as a major cause of mortality and morbidity. 11,13 The existence of E. coli O157:H7 in the contaminated vegetables sprouts and fresh juice has been reported from Europe, North America and Japan. 14 In Argentina, the first reported cases of foodborne outbreaks, associated with ready-to-eat salads, were contaminated with E. coli O157:H7.…”

Section: Discussionmentioning

confidence: 99%

Occurrence, Molecular Detection and Antibiotic Resistance Profile of Escherichia coli O157:H7 Isolated from Ready-to-Eat Vegetable Salads in Iran

Kouchakkhani¹,

Dehghan²,

Moosavy³

et al. 2016

Pharm Sci

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“…The diameter of the inhibition zones was measured. The strains were tested for their resistance to the following antimicrobials (µg per disk): ampicillin (10), cephalothin (5), colistin sulphate (25), gentamicin (10), streptomycin (10), sulphatriad (200), tetracycline (25), and cotrimoxazole (25). Isolates that were resistant to to at least one agent in three or more antimicrobials were considered to be multidrug resistant (MDR) [20].…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

“…These non-O157 pathogenic EPEC on raw vegetables have been detected in few studies. Mazaheri et al [24] reported a prevalence rate of 4% in lettuce in Iran, while in the Czech Republic, enteropathogenic E.coli were suspected in 11.1% of the isolates from imported vegetables that were found to have eae gene [25]. A variety of animals, such as cattle, goats, sheep, chickens, and pigeons are known to be vehicles of EPEC strains [26]; these, as well as human sources, could likely be the origin of the serogroups we found in our samples.…”

Section: Serotypingmentioning

confidence: 99%

Prevalence of antimicrobial-resistant Escherichia coli from raw vegetables in Lebanon

Faour-Klingbeil

Kuri

Fadlallah

et al. 2016

J Infect Dev Ctries

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Introduction: Fresh produce has been implicated in a number of documented outbreaks of foodborne illness caused by bacteria, viruses, and parasites. Shiga toxin-producing Escherichia coli (STEC) have been detected on vegetables, raising concerns about the prevalence of E. coli contamination in produce, which can take place at various points from farm to fork. This study aimed to detect the presence of STEC and multidrug-resistant (MDR) E. coli on fresh vegetables and water from different sources along the fresh produce supply chain in Lebanon. Methodology: E. coli isolates (n = 60) were group serotyped using trivalent antisera (trivalent 1 [O111+O55+O26], trivalent 2 [O86+O119+O127], trivalent 3 [O125; O126; O128], and trivalent 4 [O114+O124+O142]) and tested for stx1 and stx2 genes by polymerase chain reaction (PCR) assay. Resistance to antimicrobial agents was determined using the disk diffusion method. Results: The virulence genes stx1 and stx2 were not detected in any of the isolates. However, 60% of the isolates were MDR and predominantly observed in trivalent 2 (32%). It is postulated that the inadequate post-harvest washing contributed to transmission of antimicrobial-resistant E. coli at wholesale and retail levels. Fresh vegetables harbor MDR E. coli and their consumption poses risks of increasing the reservoir of antimicrobial resistance in the intestines of the Lebanese population. Conclusions: Greater emphasis should be placed on vigilant sanitation measures at the consumption level, and effective national risk mitigation strategies are crucial to minimize fecal contamination in the early stages of production, particularly in the post-harvest washing processes.

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Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic

Cited by 28 publications

References 23 publications

Occurrence of blaCTX-M-1,qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia

Occurrence of blaCTX-M-1,qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia

Occurrence, Molecular Detection and Antibiotic Resistance Profile of Escherichia coli O157:H7 Isolated from Ready-to-Eat Vegetable Salads in Iran

Prevalence of antimicrobial-resistant Escherichia coli from raw vegetables in Lebanon

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