Characteristics of enterotoxin-producing methicillin-resistant Staphylococcus aureus strains isolated from meat in Tehran, Iran

Rahimi, Forough; Shafiei, Rasoul

doi:10.1007/s00003-019-01239-z

Cited by 10 publications

(8 citation statements)

References 43 publications

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“…CT1 was the dominant PhP type in this study, followed by CT2, CT4, and CT8. Compared to our previous studies [3,5,10,12,17,19,20] , it was found that certain clonal PhP types are disseminating and circulating in different regions in Tehran. Moreover, the presence of MRSA strains with different antibiotic resistance and prophage patterns in a certain PhP type in this study indicated independent evolution of such clones in the community.…”

Section: Discussioncontrasting

confidence: 79%

“…In the present study, all MRSA strains were resistant to cefoxitin and also were positive for mecA gene. Several studies have already reported similar results among MRSA strains [3,5,20,25] . Although some MRSA strains are negative for mecA gene and instead harbor mecC gene, but since there is very low or no report on mecC gene in Iran, it could be concluded that detection of mecA gene could be a rapid and specific method for detecting MRSA strains in this country.…”

Section: Discussionsupporting

confidence: 59%

“…These differences in the prevalence of prophage types among MRSA strains in different samples in Iran could be due to the dissemination of different strains in various cities and hospitals worldwide. High-resolution PhP typing method is a powerful and specific method used to type S. aureus strains, and the usefulness and efficiency of PhP typing for MRSA strains have already been reported [3,5,10,12,20] . In this study, diverse PhP types consisting of 8 CTs and 8 STs were reported.…”

Section: Discussionmentioning

confidence: 99%

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Characteristics of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospital Wastewater in Tehran, Iran

Torabi

Rahimi

2021

IEM

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Hospital sewage is known as an important source of human pathogenic bacteria such as methicillin resistant Staphylococcus aureus (MRSA) strains disseminated from hospital to the environment. This study aimed to investigate the presence of MRSA in the treated outgoing wastewater collected from a referral hospital in Tehran, Iran. Materials & Methods: During 2015, sampling was carried out at two stages from a hospital wastewater. All black colonies with halos on HiCrome aureus agar medium supplemented with oxacillin were collected and identified as MRSA using specific primers for nucA and mecA genes. Isolates susceptibility to 18 antibiotics was determined according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). Bacterial typing was performed for the isolates using a combination of Phene plate (PhP) typing, prophage typing, staphylococcal cassette chromosome mec (SCCmec) and ccr typing methods. Findings: A total of 79 MRSA isolates were confirmed using specific primers and showed susceptibility to quinupristin-dalfopristin, vancomycin, chloramphenicol, and linezolid. High resistance to penicillin, ciprofloxacin, kanamycin, tobramycin, and erythromycin was reported. Sixteen PhP types consisting of eight common types (CTs) and eight single types (STs) were identified among the strains, among which CT1 was the dominant type. Also, two prophage patterns and four prophage types were identified, and all the strains were positive for SCCmec type III and ccr type 3. Conclusion:The results of this study revealed that sewage-treatment process was able to remove community-acquired MRSA (CA-MRSA) strains; however, hospital-acquired MRSA (HA-MRSA) strains were able to survive during the treatment process in this hospital.

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Section: Discussioncontrasting

confidence: 79%

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

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Characteristics of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospital Wastewater in Tehran, Iran

Torabi

Rahimi

2021

IEM

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“…In the present study we used a combination of the high resolution PhP typing method SCCmec types and showed the presence of diverse clonal groups of MRSA in DFI patients. CT2 was the predominant type and consisted of more than 41% of strains which is consistent with our previous ndings in Iran [5,13,19,31]. Strains belonging to different clonal types had various antibiotic resistance pro les indicating that members of these clones have disseminate and gained their antibiotic resistance independently.…”

Section: Discussionsupporting

confidence: 90%

Prevalence, diversity, and antimicrobial susceptibility profiles of methicillin- resistant Staphylococcus aureus among patients with diabetic foot infections in a referral hospital in Tehran, Iran

Rahimi,

Khashei,

Katouli

2023

Preprint

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The increasing incidence of methicillin-resistant Staphylococcus aureus (MRSA) is a major problem in the treatment of diabetic foot infections (DFIs). Due to the evolution of MRSA clones, and thereby inappropriate antibiotic therapy, typing of these strains in DFIs is needed to reduce their clonal dissemination in different regions. This study aimed to determine the prevalence, diversity, and antibiotic susceptibility profiles of MRSA isolated from patients with DFIs attending a referral hospital in Tehran, Iran during 2019–2020. Staphylococcal cassette chromosome mec (SCCmec) typing, ccr typing, PhP typing, and detection of Panton-Valentine Leukocidin (pvl) gene were done to explore the diversity of the strains. Antibiotic susceptibility profiles of the strains were also determined using the disk diffusion method and broth microdilution assay. Of the 238 S. aureus strains isolated, 73 were identified as MRSA. The highest antibiotic resistance was seen against ciprofloxacin (86%), followed by kanamycin and tobramycin (84%). Overall, MRSA strains were categorized into 29 antibiotypes. Also, 49.3% of strains were known as high-level oxacillin-resistant strains (MIC ≥ 256 µg/ml). SCCmec type III and type 3 ccr were detected in 86.3% of strains and they were classified as hospital-acquired (HA)-MRSA. PhP typing revealed the presence of 8 common types (CTs) and 11 single types (STs), with CT2 comprising 41.1% of the strains. Our data suggest that MRSA strains isolated from DFIs in this country could be diverse and resistant to clinically important antibiotics. In this context diabetic patients can serve as a reservoir for dissemination of these bacteria between community and clinical environments.

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“…The usefulness and capability of PhenePlate system for typing of staphylococci has been reported previously [15,19,[38][39][40] . In this study, diverse PhP types consisting of 3 CTs and 7 STs were identified; CT1 and CT2 were the most common types among the strains isolated from patients and healthy people, indicating their common origin.…”

Section: Discussionmentioning

confidence: 94%

Characterization of Biofilm Producing Staphylococcus epidermidis Strains Isolated from Patients and Healthy People

Danesh

Rahimi

2021

IEM

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Background: Staphylococcus epidermidis isolates are among the most important causes of nosocomial infections and could be classified as health threatening agents. This study aimed to determine the biofilm formation ability and clonal dissemination of S. epidermidis strains isolated from patients and healthy people in Isfahan during 2016 and 2017. Materials & Methods: A total of 139 and 123 suspected colonies of S. epidermidis were collected from different clinical specimens and the arm of healthy people, respectively. The ability to form biofilm was determined using a combination of Congo-red agar (CRA) and microtiter plate (MTP) assays. The presence of genes involved in biofilm formation was also tested by the polymerase chain reaction (PCR) test. The susceptibility of all strains to 12 antibiotics was evaluated using the disk diffusion method according to the Clinical & Laboratory Standards Institute (CLSI) guidelines. Moreover, all biofilm-producing strains were typed using PhenePlate system as well as cassette chromosome mec (SCCmec) and accessory gene regulator (agr) locus typing method. Findings: A total of 43 biofilm-producing S. epidermidis strains were identified among 107 and 123 confirmed strains isolated from hospitalized patients and healthy people, respectively; all of which were positive for aap gene, and the presence of ica operon was limited to 86 and 27% of the strains isolated from patients and healthy people, respectively. All the strains showed susceptibility to vancomycin, quinupristin-dalfopristin, and linezolid. Moreover, SCCmec Types III, IV, and V were detected among all methicillin-resistant S. epidermidis (MRSE) strains, and agr Type I was the most frequent one. Among all biofilm-positive strains, 3 common types (CTs) and 7 single types (STs) were determined; CT1 and CT2 were the most common types among the strains isolated from hospitalized patients and healthy people. Conclusion: These findings indicated the presence and persistence of diverse clone types of biofilm-producing S. epidermidis strains with common types of PhP, agr, and SCCmec in the hospital and the community of Isfahan.

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Characteristics of enterotoxin-producing methicillin-resistant Staphylococcus aureus strains isolated from meat in Tehran, Iran

Cited by 10 publications

References 43 publications

Characteristics of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospital Wastewater in Tehran, Iran

Characteristics of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospital Wastewater in Tehran, Iran

Prevalence, diversity, and antimicrobial susceptibility profiles of methicillin- resistant Staphylococcus aureus among patients with diabetic foot infections in a referral hospital in Tehran, Iran

Characterization of Biofilm Producing Staphylococcus epidermidis Strains Isolated from Patients and Healthy People

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