“…Cigarette smoke condensate (CSC) is known to be tumorigenic, and much work has been centered on the isolation of the biologically active compounds (1). Bioassays showed that a neutral subfraction of CSC, F-20, obtained by our fractionation procedure (Figure 1) had high tumorigenic activity (2)(3)(4). Since F-20 was shown to contain polynuclear aromatic hydrocarbons (PAH's) (5), the activity was attributed to the highly tumorigenic members of this class of compounds (6).…”