Characteristics of diarrheagenic Escherichia coli pathotypes among children under the age of 10 years with acute diarrhea

Shahbazi, Gholamhassan; Rezaee, Mohammad Ahangarzadeh; Nikkhahi, Farhad; Ebrahimzadeh, Saeed; Hemmati, Fatemeh; Namarvar, Behnaz Barzegarzadeh; Gholizadeh, Pourya

doi:10.1016/j.genrep.2021.101318

Cited by 8 publications

(7 citation statements)

References 43 publications

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“…The present study differed from a study in Duhok city by Hasan et al (2020), by which LT toxin solely identified in 37% of the cases [30], whereas seven strains positive for LT gene and three strains positive for ST were identified by Khalil (2015) in Bagdad [32]. [36], [37], [46], [49].…”

Section: Stable and Ltcontrasting

confidence: 97%

“…PCR-based studies detected showed different percentage rates of ETEC in stool samples ranging from 18% to 26% [30], [32], [36], [40], [41]. Results of this study were not parallel to conclusions mentioned by other investigators who reported lower percentage rates of positive results [34], [37].…”

Section: Identification Of Etec Toxinscontrasting

confidence: 80%

“…According to many reports around the world, various factors may be the primary causes of diarrheal outbreaks including; traveling to tropical zones, consuming contaminate, and lack of personal hygiene [35]. However, considering that prior studies have focused on certain aspects such as geographical conditions, sampling period, study population, hygienic level of region, and detection technologies [37].…”

Section: Detection Of Decmentioning

confidence: 99%

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Molecular detection of Enterotoxigenic Escherichia coli Toxins and Colonization Factors from Diarrheic Children in Pediatric Teaching Hospital, Sulaymaniyah, Iraq

et al. 2022

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Enterotoxigenic Escherichia coli (ETEC) is one well-established causative agent of diarrhea in the developing countries among young children. This prospective study was performed at Laboratories of University of Sulaimani (in Sulaymaniyah City/Iraq) from September to October 2021which aimed to determine the prevalence of ETEC among children and the most prevalence colonization factor (CFA/I) among ETEC. One hundred and twenty-five fresh stool samples were collected from hospitalized – children with diarrhea at Dr. Jamal Ahmed Rashid’s Pediatric Teaching Hospital. The collected samples were cultured on MacConkey and eosin methylene blue agar as selective and differential media for Gram- negative bacteria. Colonies were identified through Gram staining and biochemical tests including: Indole, methyl red, and catalase reaction test. Vitek-2 machine was depended to test some obtained isolates. Most of isolates (60%) showed positive results for E. coli – out of this percentage, 14 (18.66%) were positive for ETEC using polymerase chain reaction assay identifying stable and labile toxins (LTs). It was noticed that all of the ETEC isolates were stable toxin producer isolates whereas LT producer isolates were not identified. Colonization factor 5 (CS5) has been detected among three ETEC isolates (21.42%), meanwhile, 11 isolates (78.57%) have not expressed colonization factors at all.

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Section: Stable and Ltcontrasting

confidence: 97%

Section: Identification Of Etec Toxinscontrasting

confidence: 80%

Section: Detection Of Decmentioning

confidence: 99%

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Molecular detection of Enterotoxigenic Escherichia coli Toxins and Colonization Factors from Diarrheic Children in Pediatric Teaching Hospital, Sulaymaniyah, Iraq

et al. 2022

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“…Diarrheagenic Escherichia coli (DEC) is an important agent of epidemic diarrhea worldwide especially in some underdeveloped areas with poor sanitation among bacterial pathogens (Gomes et al, 2016). DEC can be divided into five categories according to its virulence characteristics: enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), and Shiga toxin-producing E. coli (STEC) (Shahbazi et al, 2021). Strains can be identified according to the characteristic virulence genes of different DEC categories: stx1, stx2, and eae in STEC; aggR and astA in EAEC; invE and ipaH in EIEC; est and elt in ETEC.…”

Section: Introductionmentioning

confidence: 99%

Genotyping Based on CRISPR Loci Diversity and Pathogenic Potential of Diarrheagenic Escherichia coli

et al. 2022

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Diarrheagenic Escherichia coli (DEC) can cause epidemic diarrhea worldwide. The pathogenic potential of different strains is diverse and the continuous emergence of pathogenic strains has brought serious harm to public health. Accurately distinguishing and identifying DEC with different virulence is necessary for epidemiological surveillance and investigation. Clustered regularly interspaced short palindromic repeats (CRISPR) typing is a new molecular method that can distinguish pathogenic bacteria excellently and has shown great promise in DEC typing. The purpose of this study was to investigate the discrimination of CRISPR typing method for DEC and explore the pathogenicity potential of DEC based on CRISPR types (CT). The whole genome sequences of 789 DEC strains downloaded from the database were applied CRISPR typing and serotyping. The D value (Simpson’s index) with 0.9709 determined that CRISPR typing had a higher discrimination. Moreover, the same H antigen strains with different O seemed to share more identical spacers. Further analyzing the strains CRISPR types and the number of virulence genes, it was found that there was a significant correlation between the CRISPR types and the number of virulence genes (p < 0.01). The strains with the largest number of virulence genes concentrated in CT25 and CT56 and the number of virulence genes in CT264 was the least, indicating that the pathway potential of different CRISPR types was variable. Combined with the Caco-2 cell assay of the laboratory strains, the invasion capacity of STEC strains of different CRISPR types was different and there was no significant difference in the invasion rate between different CRISPR type strains (p > 0.05). In the future, with the increase of the number of strains that can be studied experimentally, the relationship between CRISPR types and adhesion and invasion capacities will be further clarified.

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“…In this study, solvent-sensitive nanoparticles (SSNPs), a kind of stimulus-responsive nanoparticle, were first developed to enhance the efficiency of MELGA and detect pathogenic enterotoxigenic Escherichia coli (ETEC). This pathogen causes more than 400 million diarrhoeagenic cases per year, resulting in 300,000-500,000 casualties, which mostly occur in young children in developing countries [12][13][14][15][16] . Since PCR-based molecular assays are the only way to distinguish ETEC from other E. coli 12,16,17 , these assays need to be improved for efficient detection 13,18 .…”

mentioning

confidence: 99%

Solvent-sensitive nanoparticle-enhanced PCR assay for the detection of enterotoxigenic Escherichia coli

Teawprasong

Wongngam

Tangchaikeeree

et al. 2022

Sci Rep

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Stimulus-responsive nanoparticles are among the most utilized nanoscale materials in biomedical applications. As these nanoparticles exhibit a manipulable response to a particular stimulus, such as pH, heat, and organic solvent, they are potential signalling units in diagnostic assays. This study aims to enhance the limit of detection and reduce the turnaround time of magnetic nanoparticle polymerase chain reaction (PCR) enzyme-linked gene assay (MELGA), an advanced PCR-based technique termed the solvent-sensitive nanoparticle (SSNP)-enhanced PCR assay. This technique was proposed to detect pathogenic enterotoxigenic Escherichia coli (ETEC) through applying stimulus-responsive nanoparticles. The SSNPs were elaborated with three main components, including mesoporous silica nanoparticles as a structural unit, organic dye (Nile red) as a payload, and the corresponding organic solvent-sensitive polymer shell as “gatekeeper” (poly(maleic anhydride-alt-methyl vinyl ether, PMAMVE). A suitable organic solvent capable of inducing polymer swelling and dye dissolution was investigated by considering a solubility parameter. Using ethanol, the encapsulated Nile red can diffuse out of the SSNPs faster than other solvents and reach a constant concentration within 15 min. For the PCR inhibition study, various SSNPs concentrations (10–30 μg/reaction) were mixed with the ETEC gene and PCR reagent. The results showed that the particles in this concentration range did not inhibit PCR. By comparing the efficacy of conventional PCR, MELGA, and SSNP-enhanced PCR assay, the proposed technique showed a better detection limit than that of PCR, whereas that of MELGA was the lowest. Moreover, compared to MELGA or conventional PCR, this technique provided remarkably faster results in the postamplification process.

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Characteristics of diarrheagenic Escherichia coli pathotypes among children under the age of 10 years with acute diarrhea

Cited by 8 publications

References 43 publications

Molecular detection of Enterotoxigenic Escherichia coli Toxins and Colonization Factors from Diarrheic Children in Pediatric Teaching Hospital, Sulaymaniyah, Iraq

Molecular detection of Enterotoxigenic Escherichia coli Toxins and Colonization Factors from Diarrheic Children in Pediatric Teaching Hospital, Sulaymaniyah, Iraq

Genotyping Based on CRISPR Loci Diversity and Pathogenic Potential of Diarrheagenic Escherichia coli

Solvent-sensitive nanoparticle-enhanced PCR assay for the detection of enterotoxigenic Escherichia coli

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