Characteristics of diarrheagenic Escherichia coli among patients with acute diarrhea in China, 2009‒2018

Zhou, Shi-Xia; Wang, Liping; Liu, Meng-Yang; Zhang, Haiyang; Lu, Qing‐Bin; Shi, Lusha; Ren, Xiang; Wang, Yifei; Lin, Sheng-Hong; Zhang, Cuihong; Geng, Mengjie; Zhang, Xiao-Ai; Zhu, Yuliang; Li, Zhongjie; Fang, Li‐Qun; Liu, Wei; Yang, Weizhong

doi:10.1016/j.jinf.2021.08.001

Cited by 16 publications

(9 citation statements)

References 29 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could be attributed to the underdevelopment of their innate and acquired immune system, and indigent hygiene status. Contrary to the finding of this study and many other studies in Africa, a study from China documented the preponderance of DEC in adult populations more than in children [39].…”

Section: Discussioncontrasting

confidence: 99%

See 1 more Smart Citation

A Systematic review on the status of diarrheagenic Escherichia coli (DEC) pathotypes in Nigeria; the year 2000 – 2022

Tula¹,

Filgona²,

Pukuma³

2022

Cell. Mol. Biomed. Rep.

View full text Add to dashboard Cite

This systematic review appraised the current status of DEC in Nigeria from the year 2000 to August 2022 with regards to their prevalence, pathotypes distribution, and dominance within the 6 geopolitical zones. Three research databases (AJOL, PubMed, and Google Scholar) were explored for articles of interest. From the outcome of the search, 19 full-length research articles from January 2000 to August 2022 that described the prevalence of DEC by molecular technique were recruited into the study. The results show that the studies of DEC were higher in the southern (52.63%) than in the northern (47.37%) region. Based on specimen types, 47.37% (9/19) and 52.63% (10/19) articles documented DEC from non-clinical and clinical sources, respectively. From the clinical sources, 70% of the studies targeted children of less than five years of age for the detection of DEC. The mean prevalence of DEC in Nigeria stands at 18.8%. The regional prevalence varies with the highest in the SW (32.57%) and the lowest in the NC (10.07%). The relationship between the prevalence of DEC to age group, gender, sample sources (clinical or non-clinical), study design (experimental and control groups), and regional differences were examined using odd ratios and chi-square statistics. Significance differences (P=0.0001) were obtained for all the parameters except gender which shows a lack of significant difference (P=0.1129). The most prevalent DEC pathotype was EAEC pathovar (44.62%) and the least was DAEC (2.23%). The regional distribution of the DEC pathotypes varies with the type of sample (clinical and non-clinical). Hybrid pathotypes were detected and constitute 4.89 % of the total DEC detected. The most prominent hybrid detected was EAEC/ETEC (39.13%). In that order, the DEC pathotypes were mostly resistant to ampicillin, penicillin, cotrimoxazole, and tetracycline, but were mostly susceptible to imipenem, gentamycin, and ofloxacin. We, therefore, advocate regular or periodic surveillance of DEC, and their drug resistance pattern, which will be useful to clinical personnel in their choice of a treatment regimen. Article info

show abstract

Section: Discussioncontrasting

confidence: 99%

“…Other countries of the world that documented the preponderance of EAEC pathovar over other DEC pathotypes in causing diarrhoea include China [39], Iran [43], Mozambique [34], and Mexico [44].…”

Section: Discussionmentioning

confidence: 99%

A Systematic review on the status of diarrheagenic Escherichia coli (DEC) pathotypes in Nigeria; the year 2000 – 2022

Tula¹,

Filgona²,

Pukuma³

2022

Cell. Mol. Biomed. Rep.

View full text Add to dashboard Cite

show abstract

“…31 Our study is consistent with several recent epidemiological studies that have reported an increased emergence and identification of aEPEC among EPEC infections, and there is an association with diarrheal outbreaks in both developing and developed countries. 30,[32][33][34][35][36][37][38] All strains in this study were positive for the EAST1 gene and negative for the stx gene (Table 2). The EAST1 gene is originally recognized in strains of an enteroaggregative Escherichia coli (EAEC) isolated from a Chilean children's stool specimen while suffering diarrhea.…”

Section: Discussionmentioning

confidence: 72%

Virulence Characteristics, Antibiotic Resistance Patterns and Molecular Typing of Enteropathogenic Producing Escherichia coli (EPEC) Isolates in Eastern Province of Saudi Arabia: 2013–2014

Yamani¹,

Elhadi²

2022

IDR

View full text Add to dashboard Cite

Introduction The Kingdom of Saudi Arabia has limited data on enteropathogenic Escherichia coli (EPEC). Therefore, this study was undertaken to contribute to EPEC surveillance and investigate the molecular epidemiology of EPEC strains that have been implicated in human infection in King Fahd Hospital of the University (KFHU) between 2013 and 2014 in the Eastern Province of Saudi Arabia. Methods A total of 60 non-duplicate E. coli isolates associated with human gastroenteritis were included in this study. They were characterized using PCR to determine virulence genes, antimicrobial resistance patterns, and enterobacterial repetitive intergenic consensus (ERIC-PCR). Results Among the 60 strains, 20% of those examined were positive for the intimin eae and bfpA genes and identified as typical EPEC (tEPEC). Furthermore, 44 of E. coli strains tested positive for the eae gene only and revealed a high occurrence rate of 73.3% of atypical EPEC (aEPEC) within the overall examined strains. All strains were positive for the EAST1 gene, and none tested positive for the stx gene. More than 70% of EPEC strains were multi-drug resistant (MDR) and aEPEC strains with the highest proportion of this feature of MDR. ERIC-PCR fingerprint revealed a total of 19 ERIC types with eight related distinct clusters and a similarity rate cut-off with ≥90% homology from the identified isolates. Conclusion A high antibiotic resistance rate was reported for first-line antibiotics, such as ampicillin, tetracycline, nalidixic acid, trimethoprim-sulfamethoxazole, noroxin, and ciprofloxacin.

show abstract

“…Moreover, though the subtypes of stx2a , stx2c , and stx2d are usually associated with severe human diseases (Lienemann et al 2012), non‐O157 STEC isolated from diarrhoea patients in China were dominated by stx1a and stx1c subtypes (Bai et al 2016), which are accordant with our stx subtypes. In Chinese patients, the prevalence of STEC was 0.31% (Zhou et al 2021). The low STEC prevalence of cattle as the primary source of human STEC infection and the low frequency of carriage of more pathogenic stx subtypes in STEC may explain the population’s low prevalence.…”

Section: Discussionmentioning

confidence: 99%

Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Yue-hong

Liu

Han

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: There are knowledge gaps regarding STEC and EPEC strains in livestock in Jiangsu, China. This study aimed to evaluate the potential public health significance of STEC and EPEC strains isolated from livestock by determining the serotypes, virulence profiles, and genetic relationship with international STEC strains. Methods and Results:A total of 68 STEC and 37 EPEC strains were obtained from 231 faecal sheep samples and 70 faecal cattle samples. By using whole-genome sequencing (WGS) analysis, all STEC belonged to 15 O: H serotypes, and the most prevalent serotypes were O6:H10 (19.1%), O155:H21 (14.7%), and O21:H25 (10.3%). The main Shiga toxin gene subtypes detected were stx1c (41.2%), stx1a (26.5%), stx2b (14.7%), and stx2k (14.7%). Only the STEC from cattle carried eae gene. Other adherence-associated or toxin-related genes, including lpfA (70.6%), iha (48.5%), subA (54.4%), and ehxA (33.8%), were found in STEC. All EPEC strains were bfpA-negative, and the predominant eae variants were eae-β1 (62.2%), eae-ζ (21.6%), and eae-θ (8.1%). The core-genome multilocus sequence typing (cgMLST) analysis revealed nine scattered clusters in STEC and one dominant cluster in EPEC. The strains with the same serotypes, including O22:H8 and O43:H2 in the two towns, possessed a closely genomic distance. The core genome single-nucleotide polymorphism (cgSNP) showed that part of STEC strains in this study was clustered with isolates possessing the same serotypes from the Netherlands, Sweden, and Xinjiang of China. Five serotypes of STEC isolates were associated with the clinical STEC strains from databases. Conclusion:This study provided the diverse serotypes and the virulence genes profiles in STEC and EPEC strains. Local strains possessed widely diverse and scattered clusters by cgMLST. Closely genomic correlation with clinical isolates displayed that part of the STEC strains may threaten to public health.Significance and Impact of the Study: Non-O157 STEC strains act as important pathogens for human infections. This study supports the increased surveillance work of non-O157 STEC rather than just O157 STEC in this region.

show abstract

Characteristics of diarrheagenic Escherichia coli among patients with acute diarrhea in China, 2009‒2018

Cited by 16 publications

References 29 publications

A Systematic review on the status of diarrheagenic Escherichia coli (DEC) pathotypes in Nigeria; the year 2000 – 2022

A Systematic review on the status of diarrheagenic Escherichia coli (DEC) pathotypes in Nigeria; the year 2000 – 2022

Virulence Characteristics, Antibiotic Resistance Patterns and Molecular Typing of Enteropathogenic Producing Escherichia coli (EPEC) Isolates in Eastern Province of Saudi Arabia: 2013–2014

Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Contact Info

Product

Resources

About