Characteristics of deslanoside-induced modulation on jejunal contractility

Chen, Dapeng; Xiong, Yongjian; Tang, Ze-Yao; Yao, Qiying; Ye, Dongmei; Li, Shasha; Lin, Yanfu

doi:10.3748/wjg.v18.i41.5889

Cited by 5 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DP rat model induced by acetic acid (4% v/v) instillation and the CP rat model induced by cool water (4°C) gavage were used to model hyper- and hypomotility ( 18 , 19 ). The successful establishment of inflammation-related diarrhea in the DP rats and inflammation-related constipation in the CP rats was evaluated by determining the granules and moisture content of feces.…”

Section: Resultsmentioning

confidence: 99%

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders

et al. 2021

View full text Add to dashboard Cite

Intestinal inflammation frequently occurs alongside dysmotility, which is characterized by altered myosin light chain phosphorylation levels. Curcumin, an active component from the ginger family, is reported to confer anti-inflammatory effects. However, the effects of curcumin on both diarrhea and constipation associated inflammation remains to be elucidated. The present study was designed to investigate the effects of curcumin on diarrhea and constipation and to determine the related mechanisms. Sprague-Dawley rats were used to establish diarrhea and constipation models via intracolonic acetic acid (4%) instillation or cold water gavage for 2 weeks, respectively. Blood samples were collected to measure the serum levels of the cytokines TNF-α and IL-1β using ELISA kits. Western blotting was performed to measure NF-κB, RhoA, Rho-related kinase 2, phosphorylated MLC 20 , phosphorylated myosin phosphorylated target subunit 1, 130k Da-MLC kinase (MLCK), c-kit tyrosine kinase protein expression, and reverse transcription-quantitative PCR was conducted to measure MLCK expression levels. The results indicated that curcumin reversed the elevations in the pro-inflammatory cytokines IL-1β and TNF-α by inhibiting the NF-κB pathway in rats with diarrhea and constipation. The results also indicated that myosin light chain (MLC) phosphorylation in intestinal smooth muscle was positively and negatively associated with the motility of inflammation-related diarrhea and constipation in rats, respectively. Curcumin significantly reversed the increased MLC phosphorylation in the jejunum of the rats with diarrhea, significantly enhanced the reductions in inflammatory mediators, including TNF-α and IL-1β, of rats with constipation and significantly ameliorated the related hyper-motility and hypo-motility in rats with both diarrhea and constipation. In conclusion, the potential roles of the MLC kinase, c-kit tyrosine and Rho A/Rho-associated kinase 2 pathways, which are involved in curcumin-induced amelioration of inflammation-related diarrhea and constipation, were explored in the present study. Results from the present study suggested that curcumin has potential therapeutic value for treating intestinal inflammation and inflammation-related motility disorders.

show abstract

Section: Resultsmentioning

confidence: 99%

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In this study, we have hypothesized that the ENS mediates the therapeutic effects of EA in the treatment of constipation; to this end, we used protein gene product 9.5 (PGP9.5) as a marker of ENS function . Many previous studies have confirmed that persistent stimulation with 0‐4°C water can lead to gastrointestinal disorders, for instance, inhibition of jejunal and colonic movement . Therefore, in this study, we created a mouse model of constipation that was induced by persistent stimulation with 0‐4°C saline, in order to examine the ENS mechanisms involved in the effects of EA at ST 37 and ST 25.…”

Section: Introductionmentioning

confidence: 99%

Electro‐acupuncture at ST37 and ST25 induce different effects on colonic motility via the enteric nervous system by affecting excitatory and inhibitory neurons

Chaochao

Wang²,

Gong

et al. 2018

Neurogastroenterology Motil

View full text Add to dashboard Cite

show abstract

“…Preclinical researches have shown that ST 37 could increase GI transit, relieve defecation difficulty and improve life quality [ 7 – 9 ], and indicated that it is effective for constipation. Previous experiments have shown chronic and recurrent cold water irritation to stomach might cause long-term effects on bowel movements, which resulted in the GI motility, such as inhibited jejunal and colonic motility [ 10 , 11 ]. In a cold water-induced rat model of constipation, EA stimulation at ST 37 increases faecal water content, defaecation frequency and GI transit [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture at acupoint ST 37(Shangjuxu) improves function of the enteric nervous system in a novel mouse constipation model

Chaochao

Wang²,

et al. 2016

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundElectroacupuncture (EA) at acupoint ST 37 (Shangjuxu) has been used to alleviate gastrointestinal symptoms and improve gastrointestinal motility. However, the mechanisms by which EA affects the enteric nervous system (ENS) have scarcely been investigated. In this study, we investigated whether EA could improve ENS function.MethodsA constipation model was established by gastric instillation of ice-cold saline daily for 14 days. The constipated mice were divided into two groups: the model group, which was not treated, and the EA group, which received EA at ST 37 at a frequency of 2–15 HZ and an amplitude of 1 mA for 15 min a day for 3 days. A further six mice were included as a non-constipated control group. After EA treatment, intestinal propulsion and defecation time were measured. Additionally, in jejunum, ileum and proximal colon myenteric plexus, the expressions of PGP9.5 and nNOS were measured by immunohistochemistry.ResultsThe EA group demonstrated significant improvements in carbon propulsion rates and defecation time compared to model group (P < 0.05). In addition, after EA, the PGP9.5 and nNOS expression in jejunum, ileum and proximal colonic myenteric plexus was back to normal levels.ConclusionThis study suggests that EA stimulation at ST 37 is capable of ameliorating intestinal motility dysfunction, and can partly restore enteric neuron function. The ENS can participate in changes in intestinal motility by affecting inhibitory neurons.

show abstract

Characteristics of deslanoside-induced modulation on jejunal contractility

Abstract: Deslanoside-induced BR is Ca(2+) dependent and is related to cholinergic and adrenergic systems when JSMF is in low or high contractile states.

Cited by 5 publications

References 0 publications

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders

Electro‐acupuncture at ST37 and ST25 induce different effects on colonic motility via the enteric nervous system by affecting excitatory and inhibitory neurons

Electroacupuncture at acupoint ST 37(Shangjuxu) improves function of the enteric nervous system in a novel mouse constipation model

Contact Info

Product

Resources

About