Characteristics of cyclic electron transport in the cyanobacterium Phormidium laminosum

Manasse, Robert S.; Bendall, Derek S.

doi:10.1016/0005-2728(93)90240-g

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…4B and Table 2), it is possible that the NDH1 route in total is also sensitive to HgCl 2 . Mi et al 7) have reported that HgCl 2 inhibited the NDH1 route. The low sensitivity of the NDH1 route to HgCl 2 found in the present experiment might be due to remaining reductants on the reducing side of PSI under the experimental conditions, due to the presence of other reductants of NAD þ , or due to differences in the experimental conditions for the measurement of cyclic electron flow and the NAD(P)H oxidase activities.…”

Section: Activity Staining Of Proteins With Nad(p)h-oxidizing Activitmentioning

confidence: 99%

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The Expression Pattern of NAD(P)H Oxidases and the Cyclic Electron Transport Pathway around Photosystem I ofSynechocystissp. PCC6803 Depend on Growth Conditions

Ooyabu

Ohtsuka

Kashino

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Cyclic electron transport and NADH and/or NADPH (NAD(P)H)-oxidizing activities were investigated in Synechocystis sp. PCC6803 grown under various stressed conditions and in ndhB-less (M55) and ycf33-deletion mutants. Activity staining and inhibitor data suggested that the ferredoxin-quinone reductase (FQR) route is the main pathway in ycf33-deletion and highlight (300 E m À2 s À1 )-grown cells as well as in M55 cells. The FQR route was highly sensitive to HgCl 2 , but not to diphenyleneiodonium (DPI). On the other hand, cells grown under low CO 2 (0.03%) or normal (100 E m À2 s À1 , 3% CO 2 ) conditions were found perhaps to use the complex I-type NAD(P)H dehydrogenase route, which was found to be highly sensitive to DPI but not to HgCl 2 . In high-salt (0.55 M NaCl)-grown cells, the amount of ferredoxin-NADP þ oxidoreductase (FNR) increased, and the main cyclic electron flow was perhaps the FNR route. Both DPI and HgCl 2 were strong inhibitors of the FNR route.

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Section: Activity Staining Of Proteins With Nad(p)h-oxidizing Activitmentioning

confidence: 99%

“…The other pathway is from Fd to the plastoquinone pool via an unidentified component(s) designated ferredoxin-quinone reductase (FQR). 3,7) In this route, electrons go into the cyclic pathway from Fd without passing through FNR. Thus, there are two electron input sites for cyclic pathways on the acceptor side of PSI; one is FNR and the other is Fd.…”

mentioning

confidence: 99%

The Expression Pattern of NAD(P)H Oxidases and the Cyclic Electron Transport Pathway around Photosystem I ofSynechocystissp. PCC6803 Depend on Growth Conditions

Ooyabu

Ohtsuka

Kashino

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…Bendall and co-workers have analyzed cyclic electron transport in an assay system very similar to the one used in the present study (Cleland and Bendall, 1992;Manasse and Bendall, 1993). However, they monitored changes in A due to redox changes of Fd rather than of P700 and they routinely used high concentrations of reduced Fd.…”

Section: I Sc Ussl Onmentioning

confidence: 99%

“…The data presented here show that flavodoxin and Fd are similar in their ability to mediate the antimycin-insensitive pathway of cyclic electron transport, whereas Fd is more efficient in mediating the antimycinsensitive pathway. In cyanobacteria, antimycin-sensitive cyclic electron transport has not been found (Manasse and Bendall, 1993), and therefore Fd and flavodoxin may perform equally well as catalysts of cyclic electron transport in these organisms. Obviously, this suggestion needs to be tested with cyanobacterial thylakoids.…”

Section: I Sc Ussl Onmentioning

confidence: 99%

In Vitro Cyclic Electron Transport in Barley Thylakoids follows Two Independent Pathways

Scheller¹

1996

Plant Physiol.

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In vitro cyclic electron transport around PSI was studied in thylakoids isolated from barley (Hordeum vulgare L.). Redox poising was obtained by using anaerobic conditions, preillumination, and the addition of 3-(3,4-dichlorophenyI)-l,l-dimethylurea. Postillumination rates of P700+ re-reduction of 1 to 5 electrons s-' were observed, depending on the conditions. The thylakoids supported two parallel paths of cyclic electron transport that were distinguishable by differences in antimycin sensitivity, saturation characteristics, and substrate specificity. l h e pathway most sensitive to antimycin was not saturated at ferredoxin concentrations up to 50 PM, whereas the more insensitive pathway was saturated at 5 PM ferredoxin. At the lower concentration of reduced ferredoxin, the antimycin-sensitive rate of P700+ re-reduction was lower than the antimycin-insensitive rate. The lower range of reduced ferredoxin concentrations are closer to in vivo conditions. Flavodoxin is shown to mediate cyclic electron transport. Flavodoxin was less efficient in mediating the antimycin-sensitive pathway but mediated the antimycin-insensitive pathway as efficiently as ferredoxin. Antibodies raised against ferredoxin:NADP+ oxidoreductase had no effect on either pathway for re-reduction of P700+. However, the ferredoxin: NADP+ oxidoreductase inhibitor 2'-monophosphoadenosine-5'-diphosphoribose was able to inhibit the antimycin-sensitive as well as the antimycin-insensitive pathway.Traditionally, cyclic electron transport has been considered a means of providing ATP for CO, fixation and other energy-requiring processes in addition to the ATP provided by linear electron flow. The importance of cyclic electron transport in ATP synthesis is rather clear in the bundle-sheath cells of C, plants (Bendall and Manasse, 1995). In contrast, the contribution of cyclic electron transport to overall photophosphorylation has been reported to be very small in C, plants and cyanobacteria (Maxwell and

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“…This antimycin A sensitive reaction (shown as 9) has also been designated to chloroplasts. We still lack part of the information, however, on the protein(s) involved in the transfer of electrons from ferredoxin to PQ, the so called FQR activity (Manasse & Bendall 1993). Recent studies have indicated that this type of cyclic reaction in cyanobacteria may not occur at all, or only at a minor scale, a conclusion very well in line with the puzzling observations on the effectiveness of antimycin A inhibition in cyanobacteria (Jeanjean R, personal communication).…”

Section: Fnr ?mentioning

confidence: 81%

Photobiology of Bacteria

Hellingwerf¹,

Hoff²,

Matthijs³

et al. 1994

Antonie van Leeuwenhoek

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The field of photobiology is concerned with the interactions between light and living matter. For Bacteria this interaction serves three recognisable physiological functions: provision of energy, protection against excess radiation and signalling (for motility and gene expression). The chemical structure of the primary light-absorbing components in biology (the chromophores of photoactive proteins) is surprisingly simple: tetrapyrroles, polyenes and derivatised aromats are the most abundant ones. The same is true for the photochemistry that is catalysed by these chromophores: this is limited to light-induced exciton-or electron-transfer and photoisomerization.The apoproteins surrounding the chromophores provide them with the required specificity to function in various aspects of photosynthesis, photorepair, photoprotection and photosignalling. Particularly in photosynthesis several of these processes have been resolved in great detail, for others at best only a physiological description can be given.In this contribution we discuss selected examples from various parts of the field of photobiology of Bacteria. Most examples have been taken from the purple bacteria and the cyanobacteria, with special emphasis on recently characterised signalling photoreceptors in Ectothiorhodospira halophila and in Fremyella diplosiphon.

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Characteristics of cyclic electron transport in the cyanobacterium Phormidium laminosum

Cited by 7 publications

References 37 publications

The Expression Pattern of NAD(P)H Oxidases and the Cyclic Electron Transport Pathway around Photosystem I ofSynechocystissp. PCC6803 Depend on Growth Conditions

The Expression Pattern of NAD(P)H Oxidases and the Cyclic Electron Transport Pathway around Photosystem I ofSynechocystissp. PCC6803 Depend on Growth Conditions

In Vitro Cyclic Electron Transport in Barley Thylakoids follows Two Independent Pathways

Photobiology of Bacteria

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