Characteristics of Cucumber Mosaic Virus Isolates Infecting Cucurbits in Egypt

Hamza, Emad; Al-Naggar, A. M.; El-Shabrawi, Hattem M.; Tolba, I. H.

doi:10.21608/ajar.2022.266497

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…The symptoms associated with this disease are almost the same. In many cases, symptoms such as wrinkling, irregularity, and shoestrings in leaves, patchy patterns on leaves, dwarfism, vein banding, and yellowing raise the suspicion of viral infection (Lecoq and Desbiez, 2012;Hamza et al, 2022), which is consistent with what was observed in this study in cucurbit-grown areas in this study. In Iran, 100 plant samples including green bean, cucumber, eggplant, squash, tomato and watermelon plants were molecularly tested against different viral pathogens of cucubits.…”

Section: Figuresupporting

confidence: 91%

Iğdir İli Kabakgil Bitkilerinde Bazı Mozaik Hastalıklarının In Siliko ve Moleküler Analizi

GÜLLER,

USTA,

KORKMAZ

et al. 2024

Türk Tarım Ve Doğa Bilimleri Dergisi

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Cucumber mosaic cucumovirus (CMV) and Watermelon mosaic potyvirus (WMV) are plant viruses with positive single-stranded RNA genome that infect cucurbits and cause economic losses. Both viruses cause especially mosaic pattern and deformities in leaves, in cucurbit plants. The leaves of 23 melon and 28 watermelon plants showing such symptoms were sampled from different regions of Iğdır province. Samples with and without symptoms were tested by the Reverse Transcription Polymerase Chain Reaction (RT-PCR) using coat protein gene (CP)-specific primers. Polymerization tests amplified DNA fragments of the expected size for CMV and WMV. Some fragments with positive results were purified, bacterial cloned, nucleotide sequences revealed and registered in the GeneBank (NCBI). Sequence analyzes showed that it contained 593 bp and 822 bp for CMV and WMV, corresponding to the partial CP gene. Phylogenetic relationships with isolates from different geographical regions and plant material were investigated for both viruses. The generated phylogenetic tree confirmed that CMV-Iğdır isolate was in Group I and subgroup B, and WMV-Iğdır isolates were in different groups. In addition, coat proteins of virus isolates were characterized by in silico tools. In the current study, the presence WMV of and CMV in watermelon and melon was determined for the first time in Iğdır province, and group/subgroup assignments of CMV were revealed.

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Section: Figuresupporting

confidence: 91%

Iğdir İli Kabakgil Bitkilerinde Bazı Mozaik Hastalıklarının In Siliko ve Moleküler Analizi

GÜLLER,

USTA,

KORKMAZ

et al. 2024

Türk Tarım Ve Doğa Bilimleri Dergisi

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“…In the Mediterranean countries, four viruses, including CMV, watermelon mosaic virus, zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne yellow virus (CABYV), are most prevalent [7]. In pumpkin, several viruses, such as pumpkin yellow mosaic virus [8], zucchini yellow mosaic virus [9], papaya ringspot virus [10], squash leaf curl China virus [11], and CMV [12], have been reported.…”

Section: Introductionmentioning

confidence: 99%

Identification of a Cucumber Mosaic Virus from Cucurbita pepo on New Reclamation Land in Egypt and the Changes Induced in Pumpkin Plants

Shehata,

Iqbal,

Abdelbaset

et al. 2023

Sustainability

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In 2020, the leaves and fruit of 50 pumpkin plants with suspected cucumber mosaic virus (CMV) symptoms of leaf mosaic, vein yellowing, and mild leaf curling were collected from Sharq El-Owainat (Al Wadi El-Gaded governorate), a new reclamation land in Egypt. This study was aimed at deciphering and characterizing the causal agent of the leaf yellowing disease associated with pumpkin plants in Egypt. The causal agent was identified by serological, cytological, and molecular means. The serological identification by DAS–ELISA confirmed the presence of CMV in 20% of the plants. The cytological identification by electron microscopy revealed typical cucumovirus isometric particles of 28–30 nm diameter in the cytoplasm of the leaf parenchyma, epidermal cells, the integument, and the nucleus. Molecular characterization by one-step reverse transcriptase-PCR yielded the required size of amplicon (678 bp) for CMV. Additionally, mechanical sap inoculation was used to determine the host range and symptomatology of the isolated CMV in seventeen different plant species belonging to six different plant families. CMV replicated, moved systemically, and induced a range of symptoms in sixteen plant species. The isolated CMV was transmitted to pumpkin plants at a 16.4% rate by seeds. CMV-infected pumpkin plant leaves were characterized by a substantially low concentration of photosynthetic pigments, a high level of reducing sugars, relatively low protein levels, and a significant increase in total phenol contents, implying their potential role as antiviral agents. Ultrathin sections of infected cells revealed histological changes and cytological abnormalities in comparison to healthy plants. This is the first identification of CMV on new reclamation land in Egypt, pinpointing its swift spread, which could pose a major constraint to pumpkin production in Egypt.

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Characteristics of Cucumber Mosaic Virus Isolates Infecting Cucurbits in Egypt

Cited by 2 publications

References 29 publications

Iğdir İli Kabakgil Bitkilerinde Bazı Mozaik Hastalıklarının In Siliko ve Moleküler Analizi

Iğdir İli Kabakgil Bitkilerinde Bazı Mozaik Hastalıklarının In Siliko ve Moleküler Analizi

Identification of a Cucumber Mosaic Virus from Cucurbita pepo on New Reclamation Land in Egypt and the Changes Induced in Pumpkin Plants

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