Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases

Lee, Kyungwon; Kim, Chang Ki; Hong, Seong Geun; Choi, Jong-Kuk; Song, Sungwook; Koh, Eunmi; Yong, Dongeun; Jeong, Seok Hoon; Yum, Jong Hwa; Docquier, Jean Denis; Rossolini, Gian María; Chong, Yunsop

doi:10.1016/j.ijantimicag.2010.05.018

Cited by 30 publications

(24 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A. nosocomialis is the second-most-frequent species isolated from blood among the genus Acinetobacter in South Korea and displays a low carbapenem resistance rate but a high polymyxin resistance rate, unlike A. baumannii (6). In our recent study, two carbapenem-resistant A. nosocomialis isolates from South Korea contained the bla SIM-1 gene, which has been identified in other Acinetobacter species, including A. baumannii (3,4,7,12). Although bla OXA-23 has been identified in A. radioresistens and Acinetobacter baylyi (9,12), it was first identified in A. nosocomialis.…”

mentioning

confidence: 86%

AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

Kim

Choi

Jung

et al. 2012

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 86%

AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

Kim

Choi

Jung

et al. 2012

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…1). Genes encoding plasmid-borne carbapenemases of classes A, B and D were not detected in any of the isolates (Bae et al, 2011;Lee et al, 2010b;Poirel et al, 2004bPoirel et al, , 2011Smith Moland et al, 2003). Carbapenem resistance was not transferred to the recipient via conjugation in any of the 16 isolates.…”

Section: Antimicrobial Susceptibilitiesmentioning

confidence: 99%

Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36

Shin

Bae

Kim

et al. 2012

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36 This study investigated the molecular mechanisms of carbapenem resistance in clinical isolates of Klebsiella pneumoniae from Korea and the clinical outcomes of resistant K. pneumoniae infection. Sixteen K. pneumoniae isolates showing resistance to carbapenems collected from a tertiary-care hospital were examined for the mechanisms of carbapenem resistance. PCR and sequencing experiments detected the bla DHA-1 AmpC b-lactamase gene in all 16 clinical isolates, whilst the bla genes of extended-spectrum b-lactamases were detected in 12 of 16 isolates. SDS-PAGE experiments indicated that all the isolates lacked the 35 kDa and/or 36 kDa outer-membrane proteins (OMPs). Sequence analysis of the corresponding OMP genes revealed various alterations. PFGE analysis demonstrated that there were no major clonal relationships among the K. pneumoniae isolates. However, multilocus sequence typing experiments showed that all isolates shared the same sequence type (ST), ST11, except for one isolate of ST48. The crude mortality rate of infected patients was 81.8 %. Carbapenem resistance was mainly due to a combination of DHA-1 AmpC b-lactamase coupled with the loss of OmpK35 and/or OmpK36 and was associated with poor clinical outcome. INTRODUCTIONCarbapenems are widely used and are often the only therapeutic option for treating serious infections caused by Klebsiella pneumoniae that produces extended-spectrum b-lactamases (ESBLs) and/or AmpC b-lactamases. The recent emergence of carbapenem-resistant K. pneumoniae in various countries is therefore worrying, as antimicrobial treatment options are limited.Carbapenem resistance in K. pneumoniae has been ascribed mainly to enzymic degradation by plasmid-borne carbapenemases of classes A (GES-2, -4, -5 and -6, and KPC enzymes), B (IMP-1 and -8, and VIM-1, -2 and -12) and D (OXA-48) (Poirel et al., 2004a;Queenan & Bush, 2007). However, some K. pneumoniae strains have acquired carbapenem resistance despite their lack of carbapenemase production. Loss of outer-membrane proteins (OMPs), including OmpK35 and OmpK36, plays a role in carbapenem resistance in K. pneumoniae strains harbouring ESBLs METHODSPatients and strains. This study was conducted between 2007 and 2008 at a tertiary-care teaching hospital with 2000 beds in Seoul, Korea. During this period, K. pneumoniae was isolated from 3278 patients, 16 (0.5 %) of which had K. pneumoniae clinical isolates that exhibited resistance to carbapenems. The isolates were identified using a Vitek GNI card (bioMérieux). Clinical characteristics were investigated through electronic medical record review. Data including age, sex, underlying disease, number of days from hospital admission to identification of K. pneumoniae infection, prior antibiotic treatment, anatomical site of isolation and clinical outcome were obtained.Antimicrobial susceptibility testing. Antibiotic-containing discs (Becton Dickinson) were used for routine an...

show abstract

“…bla SIM-1 was confirmed to have a chromosomal location in Acinetobacter spp. of South Korea (10,11), but it has a large-plasmid location in A. baylyi strain NB09A30. Using the A. baumannii plasmid replicon typing scheme constructed by Bertini et al (4), we detected four replicase (rep) gene groups (GRs), GR2, GR3, GR7, and GR13, in A. baylyi strain NB09A30.…”

mentioning

confidence: 99%

Clinical Carbapenem-Resistant Acinetobacter baylyi Strain Coharboring bla _SIM-1 and bla _OXA-23 from China

Zhou

Wang

et al. 2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

bla SIM-1 and bla OXA-23 were codetected in clinical carbapenem-resistant Acinetobacter baylyi strain NB09A30. Both of carbapenemase genes were located on a large plasmid (ca. 360 kb). bla SIM-1 was found as a gene cassette inserted into a class 1 integron identical to that determined in Acinetobacter sp. isolates from South Korea. The genetic structure of bla OXA-23 in NB09A30 was different from that in the prevalent Acinetobacter baumannii of clonal complex 92 (CC92) from the same hospital.Carbapenem-resistant Acinetobacter spp. are one of the most frightening threats in the current antibiotic era (14). The mechanisms of Acinetobacter sp. resistance to carbapenem are production of carbapenemases (the main mechanism), overexpression of efflux pumps, and reduced expression or loss of outer membrane proteins (8). The metallo-␤-lactamase (MBL) gene, bla SIM-1 , was first identified in Acinetobacter spp. from South Korea in 2005 (11) and then in Acinetobacter genomic species 10 from two hospitals of South Korea in 2010 (10) but, as far as we know, has not yet been detected in other countries. Here, we report the emergence of Acinetobacter baylyi coharboring bla SIM-1 and bla in China.A 59-year-old male was admitted for cerebral hemorrhage at the Affiliated Hospital of Ningbo University in June 2009. After surgery to remove the intracerebral hematoma, he was transferred to the intensive care unit and received antibiotic therapy with cefminox, which was later replaced with cefodizime plus amikacin. Imipenem-resistant Acinetobacter sp. NB09A30 was isolated from the cloudy secretion of the surgical incision 10 days after the operation. MICs were determined by Etest and interpreted according to the CLSI 2011 recommendation (7). The strain was resistant to almost all ␤-lactams, including imipenem (MIC Ն 32 g/ml) and meropenem (MIC Ն 32 g/ml), but remained susceptible to minocycline, tigecycline, ciprofloxacin, levofloxacin, and colistin. The MICs of gentamicin and amikacin were Ն256 g/ml and 16 g/ml, respectively. Etest MBL strip testing against NB09A30 indicated the presence of metallo-␤-lactamase (imipenem/imipenem plus EDTA MIC of Ͼ16 g/ml). Surgical site infection was suspected, and the antibiotic regimen was changed to levofloxacin. The man was cured finally, and NB09A30 was no longer recovered.PCR detection of the bla OXA-51 -like gene in NB09A30 was negative, implying that NB09A30 is not an Acinetobacter baumannii strain (15). The 16S-23S rRNA intergenic spacer region (ISR) of NB09A30 was amplified and cloned into the pGEM-T Easy vector for sequencing (5). This showed that NB09A30 possessed ISRs with different lengths (long ISR, 650 bp; short ISR, 611 bp), a unique organization of the 16S-23S rRNA ISRs of A. baylyi (12). The ISR sequences were 94% identical to those of A. baylyi strain 93A2 (GenBank accession no. EU042163) and A. baylyi ADP1 (GenBank accession no. CR543861); they were less than 85% identical to those of the other genomic species, which indicated that NB09A30 is an A. baylyi strain (12). PCR was p...

show abstract

Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases

Cited by 30 publications

References 13 publications

AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36

Clinical Carbapenem-Resistant Acinetobacter baylyi Strain Coharboring bla _SIM-1 and bla _OXA-23 from China

Contact Info

Product

Resources

About

Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases

Cited by 30 publications

References 13 publications

AbaR4-Type Resistance Island Including the bla OXA-23 Gene in Acinetobacter nosocomialis Isolates

AbaR4-Type Resistance Island Including the bla OXA-23 Gene in Acinetobacter nosocomialis Isolates

Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36

Clinical Carbapenem-Resistant Acinetobacter baylyi Strain Coharboring bla SIM-1 and bla OXA-23 from China

Contact Info

Product

Resources

About

AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

Clinical Carbapenem-Resistant Acinetobacter baylyi Strain Coharboring bla _SIM-1 and bla _OXA-23 from China