Characteristics of cells isolated from the medullary thick ascending limb of henle's loop

Eveloff, Jill; Bayerdörffer, Eckehard; Kinne, Rolf

doi:10.1016/0020-711x(79)90164-2

Cited by 2 publications

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“…Cell populations, viable for culture, may be isolated successfully by taking advantage of physicochemical differences between renal cell types for separation on continuous gradients [33] or by free-flow electrophoresis [46,63]. Once segmental cell strains or cell lines have been established, renal cells in culture could serve to elucidate problems posed by the diseased kidney.…”

Section: Perspectives In Renal Tissue Culturementioning

confidence: 99%

Tissue culture in nephrology: Potential and limits for the study of renal disease

Horster

1980

Klin Wochenschr

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Kidney cells, when isolated and cultivated in vitro, retain differentiated renal properties. Glomerular epithelial and mesangial cells from animal and human kidneys express their normal ultrastructure and the ability for basement membrane biosynthesis. Mesangial cells in culture have been utilized particularly for the study of hormonal tissue receptors, of prostaglandin production, and of their contractile response to various hormonal stimuli. Cells of tubule origin have been a valuable tool for the study of transport mechanism which, as a consequence of the heterogeneity of nephron functions, can not be assessed in vivo. Ion transport and its structural basis, as well as transport regulation by hormones has been studied in established epithelial cell lines. Induction of ion transport and enzyme activities, and the control of cell proliferation and differentiation has also been succesfully evaluated in cultured epithelia derived from the kidney. Future work will attempt to prepare cell lines from defined nephron segments to study chemical and physical phenomena of renal disease.

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Section: Perspectives In Renal Tissue Culturementioning

confidence: 99%

Tissue culture in nephrology: Potential and limits for the study of renal disease

Horster

1980

Klin Wochenschr

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Selective distal nephron damage during isolated kidney perfusion

Alcorn

Emslie

Ross

et al. 1981

Kidney International

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The morphologic characteristics of the isolated perfused rat kidney were examined by light and electron microscopy. Following perfusion for 90 min, the cortex appeared normal except for the development of spaces in glomerular mesangial regions and ultrastructural abnormalities in very few proximal tubular cells. In the medulla, descending limbs of the loops of Henle and Collecting tubules appeared normal. During perfusion, however, even under optimal conditions, consistent and reproducible changes rapidly developed in cells of the thick ascending limb of Henle's loop, more specifically, in cells of the straight portion of the distal tubule in the inner stripe of the outer medulla. The changes became progressively more severe as perfusion continued and ranged from swelling of mitochondria at 15 min to complete cellular disruption by 90 min. Horseradish peroxidase added to the perfusion medium was found in and between damaged cells and in the interstitial tissue adjacent to affected areas. The pathogenesis of these selective medullary distal tubular abnormalities may relate to the specialized functional activity of this segment of the nephron. Such abnormalities may contribute to the specific functional defects of the preparation.

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Characteristics of cells isolated from the medullary thick ascending limb of henle's loop

Cited by 2 publications

References 0 publications

Tissue culture in nephrology: Potential and limits for the study of renal disease

Tissue culture in nephrology: Potential and limits for the study of renal disease

Selective distal nephron damage during isolated kidney perfusion

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