Characteristics of Bupleurum falcatum plants propagated through somatic embryogenesis of callus cultures

Abstract

Various characteristics including the saponin content in the root of Bupleurum falcatum plants propagated in vitro through somatic embryogenesis of callus cultures were compared with those of the plants propagated by seeds. The asexually propagated plants had an aerial part of more uniform characteristics than those of sexually propagated ones. However, both the mean and variance of root weight of the former were significantly larger than those of the latter. As for the saponin content of the root on a dry weight basis, there was little difference between the two groups. The amounts of saikosaponins c and d in a root were significantly larger in the asexually propagated plants than in the sexually propagated ones.

Somatic embryogenesis and plant regeneration from callus cultures of Aconitum heterophyllum Wall

“…The procedure of obtaining plantlets from somatic embryos by using low or no salts in the culture medium with I mg l-1 IBA or by dipping the embryos in a concentrated solution of IBA (1 mg m1-1) is important as an approach to propagation via callus induced somatic embryogenesis. Similar studies in medicinal plant species namely Foeniculum vulgate (Miura et al 1987) and Bupleurum falcatum (Hiraoka et al 1986) have shown that the coefficient of variation between plants obtained via embryogenesis was lower when compared with plants obtained from seeds. Further, the productivity in terms of quantity as well as quality of secondary metabolites was enhanced in plants derived from somatic embryos.…”

Haploid Plantlet Production through Somatic Embryogenesis in Anther-Derived Callus ofBupleurum falcatum

“…In B. falcatum, somatic embryos were induced by the culture of tissues other than anther culture (Ahn et al, 1999;Amano et al, 1989;Fujioka et al, 1987;Hiraoka et al, 1986;Park et al, 1994;Xia et al, 1992), but we reported plantlet regeneration and haploid production through anther culture of B. falcatum as a short communication . The present study was made to verify the haploid plant production using anther donors of different geographical origins and to elucidate the induction of plant regeneration through somatic embryogenesis from anther-derived callus in B. falcatum.…”

“…In B. falcatum, some studies on tissue culture using other parts of plants; i.e., root, leaf and shoot apex have been reported (Ahn et al, 1999;Amano et al, 1989;Fujioka et al, 1987;Hiraoka et al, 1986;Park et al, 1994;Xia., 1992). In the anther culture system, there are usually large variations in callus formation and shoot or embryo production.…”

Application of Plant Cell and Tissue Culture for the Production of Phytochemicals in Medicinal Plants

“…Many important medicinal herbs have been successfully propagated in vitro since long before either by organogenesis [10][11][12][13][14][15][16] or by somatic embryogenesis [17][18][19]. In vitro propagated plants of many important medicinal species were found to be uniform, showing less variation in their content of secondary metabolites than their wild/cultivated counterparts [20].…”