Characteristics of Biofilm Formation Process in the Bioelectrochemical Systems, Working in Batch Mode of Cultivation

Zubchenko, Liudmyla; Kuzminskiy, Yevhen

doi:10.23939/chcht11.01.105

Cited by 2 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The isolation of a novel electrogenic strain NIT-T3 of the genus Desulfuromonas and its respiration-ability specific to solid minerals suggests the adaptation of the genus to solid minerals or conductor-driven metabolism [ 8 , 9 , 10 , 11 , 12 , 13 ]. The ability to grow on solid minerals and electrodes is supported by the presence of seven extracellular c -type cytochromes ( Figure 5 ), and conductive pilin (PilA)-homologs and full sets of T4P-assembly genes in the genome ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Polyphasic Characterization of Desulfuromonas versatilis sp. Nov., an Electrogenic Bacteria Capable of Versatile Metabolism Isolated from a Graphene Oxide-Reducing Enrichment Culture

et al. 2021

View full text Add to dashboard Cite

In this study, a novel electrogenic bacterium denoted as strain NIT-T3 of the genus Desulfuromonas was isolated from a graphene-oxide-reducing enrichment culture that was originally obtained from a mixture of seawater and coastal sand. Strain NIT-T3 utilized hydrogen and various organic acids as electron donors and exhibited respiration using electrodes, ferric iron, nitrate, and elemental sulfur. The strain contained C16:1ω7c, C16:0, and C15:0 as major fatty acids and MK-8, 9, and 7 as the major respiratory quinones. Strain NIT-T3 contained four 16S rRNA genes and showed 95.7% similarity to Desulfuromonasmichiganensis BB1T, the closest relative. The genome was 4.7 Mbp in size and encoded 76 putative c-type cytochromes, which included 6 unique c-type cytochromes (<40% identity) compared to those in the database. Based on the physiological and genetic uniqueness, and wide metabolic capability, strain NIT-T3 is proposed as a type strain of ‘Desulfuromonas versatilis’ sp. nov.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The genus Desulfuromonas has received much attention as a common electrogenic bacteria present in BESs [ 8 , 9 , 10 ]. Desulfuromonas species are found in natural environments, such as aquifers [ 11 ], sediment cores [ 12 ], and terrestrial mud volcanoes [ 13 ], suggesting a wide distribution.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Polyphasic Characterization of Desulfuromonas versatilis sp. Nov., an Electrogenic Bacteria Capable of Versatile Metabolism Isolated from a Graphene Oxide-Reducing Enrichment Culture

et al. 2021

View full text Add to dashboard Cite

show abstract

“…There is a certain number of techniques for formation of biofilm with exoelectrogenic activity. They are based on supporting of conditions that can promote the development of exoelectrogens and suppress other bacteria [13][14][15][16][17].…”

mentioning

confidence: 99%

High Exoelectrogenic Biofilms Formation in Microbial Fuel Cells

Shchurska

Zubchenko

Sobczuk

et al. 2019

Innov Biosyst Bioeng

Self Cite

View full text Add to dashboard Cite

Background. Microbial fuel cells are devices in which electricity is generated by microorganisms called exoelectrogens. During the process of anaerobic respiration exoelectrogens emit electrons outside the cell. These electrons can be transferred to the anode of biofuel cell via several different mechanisms. Electricity generation in microbial fuel cells depends primarily on the electrochemical activity of the exoelectrogens present in the anode space. Nowadays, the usage of microorganisms, immobilized as biofilms on the anode, is constantly increasing. Natural sources for exoelectrogens selection such as activated sludge, biofilter biofilms, sediments of seas and rivers have a very diverse microbial composition. Therefore, it is important to immobilize relatively deficient in natural sources exoelectrogens on the anode during the biofilm formation process. The main research areas are the development of a technique for obtaining of electroactive biofilms enriched with exoelectrogens along with reduction of the period of biofilm formation process. Objective. We set a goal to study the process of high exoelectrogenic biofilm formation basing on the combination of different methods of exoelectrogens isolation and immobilization at the anode of a microbial fuel cell. Methods. A three-stage technique was used to obtain a highly exoelectrogenic biofilm which, due to the combination of typical isolation and immobilization techniques of exoelectrogens, allows obtaining the biofilm in which the vast majority of microorganisms are exoelectrogens. In the first stage, a biofilter biofilm was used as a source of exoelectrogens. The biofilm formed in the first stage was used as an inoculum for the second stage of biofilm formation. During the second stage an additional selective factor (applied additional potential in the electrical circuit of the microbial fuel cell) was used. The third stage of biofilm formation was the isolation of exoelectrogens capable of reducing ferum (III) compounds from secondary biofilm with subsequent application of these cells as inoculum.Results. The usage of the proposed method allows obtaining of a biofilm enriched with exoelectrogenic bacteria. The maximum current density generated by the biofilm, obtained during the first stage, reaches 140 μA/cm 2 , during the second -400 μA/cm 2 , during the third -615 μA/cm 2 . The duration of biofilm formation at each stage was 110 h, 40 h, and 60 h, respectively. Conclusions. It has been proven that the duration of biofilm formation is reduced almost twice as a result of a combination of typical methods of isolation and immobilization of exoelectrogens; obtained biofilm has high electrochemical activity and properties similar to biofilm, formed by pure cultures of exoelectrogens.

show abstract

Characteristics of Biofilm Formation Process in the Bioelectrochemical Systems, Working in Batch Mode of Cultivation

Cited by 2 publications

References 11 publications

Isolation and Polyphasic Characterization of Desulfuromonas versatilis sp. Nov., an Electrogenic Bacteria Capable of Versatile Metabolism Isolated from a Graphene Oxide-Reducing Enrichment Culture

Isolation and Polyphasic Characterization of Desulfuromonas versatilis sp. Nov., an Electrogenic Bacteria Capable of Versatile Metabolism Isolated from a Graphene Oxide-Reducing Enrichment Culture

High Exoelectrogenic Biofilms Formation in Microbial Fuel Cells

Contact Info

Product

Resources

About