Characteristics of bio-CaCO3 from microbial bio-mineralization with different bacteria species

Qian, Chunxiang; Ren, Xinwei; Yang, Rui; Wang, Kai

doi:10.1016/j.bej.2021.108180

Cited by 33 publications

(5 citation statements)

References 28 publications

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“…After biosorption and biomineralization, amorphous Ca-Tb mixed phases were gradually generated on the cell surface, accompanied by an enhancement in the intensity of the Tb elemental signal, which was consistent with the results of existing studies ( Fan et al, 2020 ; Qian et al, 2021 ; Dong et al, 2022 ). However, comparing the energy spectra after 10 min of adsorption ( Figures 9B , C ), the Tb signal intensity was much lower than that after 10 min of MICP, suggesting that MICP had an obvious facilitating role in the recovery of Tb 3+ .…”

Section: Resultssupporting

confidence: 91%

Recovery of terbium by Lysinibacillus sp. DW018 isolated from ionic rare earth tailings based on microbial induced calcium carbonate precipitation

Bian,

Dong,

Ning

et al. 2024

Front. Microbiol.

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Microbial induced calcium carbonate precipitation (MICP) is considered as an environmentally friendly microbial-based technique to remove heavy metals. However, its application in removal and recovery of rare earth from wastewaters remains limited and the process is still less understood. In this study, a urease-producing bacterial strain DW018 was isolated from the ionic rare earth tailings and identified as Lysinibacillus based on 16S rRNA gene sequencing. Its ability and possible mechanism to recover terbium was investigated by using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and fourier transform infrared spectroscopy (FTIR). The results showed that the urease activity of DW018 could meet the biomineralization requirements for the recovery of Tb3+ from wastewaters. The recovery rate was as high as 98.28% after 10 min of treatment. The optimal conditions for mineralization and recovery were determined as a bacterial concentration of OD600 = 1.0, a temperature range of 35 to 40°C, and a urea concentration of 0.5%. Notably, irrespective of CaCO3 precipitation, the strain DW018 was able to utilize MICP to promote the attachment of Tb3+ to its cell surface. Initially, Tb3+ existed in amorphous form on the bacterial surface; however, upon the addition of a calcium source, Tb3+ was encapsulated in calcite with the growth of CaCO3 at the late stage of the MICP. The recovery effect of the strain DW018 was related to the amino, hydroxyl, carboxyl, and phosphate groups on the cell surface. Overall, the MICP system is promising for the green and efficient recovery of rare earth ions from wastewaters.

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Section: Resultssupporting

confidence: 91%

Recovery of terbium by Lysinibacillus sp. DW018 isolated from ionic rare earth tailings based on microbial induced calcium carbonate precipitation

Bian,

Dong,

Ning

et al. 2024

Front. Microbiol.

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“…Moreover, the deposition of CaCO 3 was found to be quantitatively enhanced under conditions involving a calcium chloride concentration of 0.4-0.6 mmol/L, urea concentration of 0.8-1.0 mmol/L, temperature of 30°C, and pH range of 7-9, as depicted in Supplementary Figure S1. Those findings aligned with the optimal temperature and pH values reported in the earlier research works for the other urease-producing bacteria, and strain NS-6 exhibited significantly high urease activity at 32 h of cultivation compared to previously reported urease-producing bacteria (Qian et al, 2021;Song et al, 2022;Diez-Marulanda and Brandao, 2023).…”

Section: Identification and Characteristics Of Strain Ns-6supporting

confidence: 90%

“…Herein, Neobacillus mesonae strain NS-6 that possessed urease-producing capabilities and exhibited tolerance to alkaline environments, might potentially serve as a novel bacterial species for facilitating MICP. The spherical shape of CaCO 3 crystals corresponded to vaterite, and the rhombohedral shape corresponded to calcite by strain NS-6, different from calcite mainly formed by other reported bacteria (Qian et al, 2021). Additionally, the expanded perlite particles exhibited numerous cavities, as observed through FESEM and XRD analysis.…”

Section: Discussionmentioning

confidence: 77%

Characterization and genome analysis of Neobacillus mesonae NS-6, a ureolysis-driven strain inducing calcium carbonate precipitation

Xu,

Zhang,

et al. 2023

Front. Microbiol.

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In this study, a highly promising bacterium was isolated from sandstone oil in the Ordos Basin, named strain NS-6 which exhibited exceptional urease production ability and demonstrated superior efficiency in inducing the deposition of calcium carbonate (CaCO3). Through morphological and physiochemical characteristics analysis, as well as 16S rRNA sequencing, strain NS-6 was identified as Neobacillus mesonae. The activity of urease and the formation of CaCO3 increased over time, reaching a maximum of 7.9 mmol/L/min and 184 mg (4.60 mg/mL) respectively at 32 h of incubation. Scanning Electron Microscopy (SEM) revealed CaCO3 crystals ranging in size from 5 to 6 μm, and Energy Dispersive X-ray (EDX) analysis verified the presence of calcium, carbon, and oxygen within the crystals. X-ray Diffraction (XRD) analysis further confirmed the composition of these CaCO3 crystals as calcite and vaterite. Furthermore, the maximum deposition of CaCO3 by strain NS-6 was achieved using response surface methodology (RSM), amounting to 193.8 mg (4.845 mg/mL) when the concentration of calcium ions was 0.5 mmol/L supplemented with 0.9 mmol/L of urea at pH 8.0. Genome-wide analysis revealed that strain NS-6 possesses a chromosome of 5,736,360 base pairs, containing 5,442 predicted genes, including 3,966 predicted functional genes and 1,476 functionally unknown genes. Genes like ureA, ureB, and ureC related to urea catabolism were identified by gene annotation, indicating that strain NS-6 is a typical urease-producing bacterium and possesses a serial of genes involved in metabolic pathways that mediated the deposition of CaCO3 at genetic level.

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“…It has been reported that many microorganisms, including Bacillus pasteurii, Bacillus mucilaginosus, Bacillus alcalophilus, Photosynthetic bacteria could induce CaCO 3 formation except C. butyricum [ 46 , 71 , 72 ]. The CO 2 released by C. butyricum could undoubtedly react with Ca(OH) 2 , resulting in the formation of CaCO 3 .…”

Section: Resultsmentioning

confidence: 99%

In situ carbon dioxide capture to co-produce 1,3-propanediol, biohydrogen and micro-nano calcium carbonate from crude glycerol by Clostridium butyricum

Wang

Zhou

Liu

et al. 2022

Biotechnol Biofuels

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Background Climate change caused by greenhouse gas emission has become a global hot topic. Although biotechnology is considered as an environmentally friendly method to produce chemicals, almost all biochemicals face carbon dioxide emission from inevitable respiration and energy metabolism of most microorganisms. To cater for the broad prospect of biochemicals, bioprocess optimization of diverse valuable products is becoming increasingly important for environmental sustainability and cleaner production. Based on Ca(OH)2 as a CO2 capture agent and pH regulator, a bioprocess was proposed for co-production of 1,3-propanediol (1,3-PDO), biohydrogen and micro-nano CaCO3 by Clostridium butyricum DL07. Results In fed-batch fermentation, the maximum concentration of 1,3-PDO reached up to 88.6 g/L with an overall productivity of 5.54 g/L/h. This productivity is 31.9% higher than the highest value previously reports (4.20 g/L/h). In addition, the ratio of H2 to CO2 in exhaust gas showed a remarkable 152-fold increase in the 5 M Ca(OH)2 group compared to 5 M NaOH as the CO2 capture agent. Green hydrogen in exhaust gas ranged between 17.2% and 20.2%, with the remainder being N2 with negligible CO2 emissions. During CO2 capture in situ, micro-nano calcite particles of CaCO3 with sizes in the range of 300 nm to 20 µm were formed simultaneously. Moreover, when compared with 5M NaOH group, the concentrations of soluble salts and proteins in the fermentation broth of 5 M Ca(OH)2 group were notably reduced by 53.6% and 44.1%, respectively. The remarkable reduction of soluble salts and proteins would contribute to the separation of 1,3-PDO. Conclusions Ca(OH)2 was used as a CO2 capture agent and pH regulator in this study to promote the production of 1,3-PDO. Meanwhile, micro-nano CaCO3 and green H2 were co-produced. In addition, the soluble salts and proteins in the fermentation broth were significantly reduced. Graphical Abstract

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Characteristics of bio-CaCO3 from microbial bio-mineralization with different bacteria species

Cited by 33 publications

References 28 publications

Recovery of terbium by Lysinibacillus sp. DW018 isolated from ionic rare earth tailings based on microbial induced calcium carbonate precipitation

Recovery of terbium by Lysinibacillus sp. DW018 isolated from ionic rare earth tailings based on microbial induced calcium carbonate precipitation

Characterization and genome analysis of Neobacillus mesonae NS-6, a ureolysis-driven strain inducing calcium carbonate precipitation

In situ carbon dioxide capture to co-produce 1,3-propanediol, biohydrogen and micro-nano calcium carbonate from crude glycerol by Clostridium butyricum

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