Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020

Abstract

Objective: to study the bacterial microflora in the sputum of patients with pneumonia caused by SARSCoV-2 or other pathogens.Materials and methods. The bacterial microflora of sputum of 173 patients with pneumonia admitted to hospitals in Khabarovsk and the Khabarovsk Territory in May – June 2020 was examined. Detection of RNA of the SARS-CoV-2 virus was carried out by PCR with the Vector-PCRRV-2019-nCoV-RG test system (manufactured by the State Scientific Center of VB “Vector”, Koltsovo). Determination of the DNA of mycoplasmosis agents and chlamydia was carried out with the test system “AmpliSens® Mycoplasma pneumoniae/Chlamydophila pneumonia” (manufactured by CRIE). Statistical data processing was performed using the Excel program. Results and discussion. Both groups of patients (Covid-19+ and Covid-19–) had high levels of bacterial flora isolation (81.4 and 74.7 %) including common pathogens for community-acquired pneumonia as well as notable detection frequency of Candida spp. and microbial associations. The group of Covid-19+ patients demonstrated a wider range of detected pathogens, was positive for poly-resistant gram-negative Enterobacteriaceae, non-fermenting gram-negative poly-resistant bacteria, with more expressed manifestation of microbial associations. In the group of Covid-19– participants drug-resistant microflora was presented only by MRSA and MRSE staphylococci.

Microbial Landscape and Antibiotic-Susceptibility Profiles of Microorganisms in Patients with Bacterial Pneumonia: A Comparative Cross-Sectional Study of COVID-19 and Non-COVID-19 Cases in Aktobe, Kazakhstan

“…In line with previous studies, S. aureus was categorized as an emerging copathogen in individuals affected by COVID-19 [25]. The isolation of Candida fungi from the sputum of COVID-19 patients also has been reported by other authors [20,[26][27][28][29][30]. There is a significant need for a cautious interpretation of fungal isolates from respiratory samples, especially in non-critically ill patients with a low pre-test probability of invasive fungal infections due to poor oral hygiene [31][32][33], immune dysregulation [34], and viral cytopathic effects on epithelial cells [35].…”

Comparative Analysis of the Pathogen Structure in Patients with Community-Acquired and Nosocomial Pneumonia in Medical Organizations of the Rostov, Tyumen Regions and Khabarovsk Territory at the Current Stage of a New Coronavirus Infection Pandemic

“…особого внимания заслуживает тот факт, что уже при поступлении больных в инфекционный госпиталь они имеют симптомы бактериальной вп. анализ результатов изучения этиологической структуры вп, проведенный специалистами-микробиологами в ростовской, тюменской областях и хабаровском крае, четко продемонстрировал, что среди этиологических агентов вп превалируют патогены, которые по биологическим свойствам относятся к так называемым возбудителям внутрибольничных инфекций [4,6,7]. основной характеристикой таких бактерий является их поли-или панантибиотикорезистентность.…”