Characteristics of bacterial community and ARG profiles in the surface and air environments in a spacecraft assembly cleanroom

Lu, Yueying; Yang, Jianlou; Zhang, Lantao; Chen, Fangqi; Han, Pei; Fu, Yuming

doi:10.1016/j.envpol.2023.121613

Cited by 3 publications

(3 citation statements)

References 64 publications

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“…Plenty of tetracycline-resistant bacteria have been found in AS, and tetracycline resistance increases with an increasing tetracycline concentration. AS and sequencing batch reactors (SBRs) dramatically depresses the abundance of ARGs (namely, van A, ere A, amp C, aac C1, tet A and sul I) 61 , the removal rates were 2.4–4.2 log and 1.7–3.6 log, respectively. However, absolute abundance of ARG measured from anaerobic sludge (4.3 × 10 −1 ) was lower than that in aerobic sludge (3.7 × 10 0 ).…”

Section: Resultsmentioning

confidence: 99%

Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Zhao,

Li,

2024

Sci Rep

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Previous studies reported microplastics (MPs), antibiotics, and antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs). There is still a lack of research progress on the origin, fate, impact and hazards of MPs and ARGs in WWTPs. This paper fills a gap in this regard. In our search, we used “microplastics”, “antibiotic resistance genes”, and “wastewater treatment plant” as topic terms in Web of Science, checking the returned results for relevance by examining paper titles and abstracts. This study mainly explores the following points: (1) the origins and fate of MPs, antibiotics and ARGs in WWTPs; (2) the mechanisms of action of MPs, antibiotics and ARGs in sludge biochemical pools; (3) the impacts of MPs in WWTPs and the spread of ARGs; (4) and the harm inflicted by MPs and ARGs on the environment and human body. Contaminants in sewage sludge such as MPs, ARGs, and antibiotic-resistant bacteria enter the soil and water. Contaminants can travel through the food chain and thus reach humans, leading to increased illness, hospitalization, and even mortality. This study will enhance our understanding of the mechanisms of action among MPs, antibiotics, ARGs, and the harm they inflict on the human body.

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Section: Resultsmentioning

confidence: 99%

Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Zhao,

Li,

2024

Sci Rep

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“…This suggests that SAF environments may enrich for organisms with great potential for successful survival or even colonization following forward contamination by spacecraft. The microbial populations of SAFs have been described previously by classical cultivation and by molecular analyses to reveal complex communities (Favero et al, 1966;Favero, 1971;Foster and Winans, 1975;Puleo et al, 1977;Moissl et al, 2008;La Duc et al, 2009;Stieglmeier et al, 2009;Ghosh et al, 2010;Probst et al, 2010;Bashir et al, 2016;Hendrickson et al, 2017Hendrickson et al, , 2021Probst and Vaishampayan, 2020;Danko et al, 2021;Smith et al, 2022;Highlander et al, 2023;Lu et al, 2023). The microbial communities appear to be dominated by bacteria, with fewer fungi and archaea.…”

Section: Discussionmentioning

confidence: 95%

“…In molecular libraries, representatives of anaerobes and facultative strains of Firmicutes, actinobacteria and Gammaproteobacteria were observed. The most common Firmicutes, included the genera Bacillus, Clostridium, Enterococcus, Lactobacillus, Paenibacillus, and Staphylococcus (Moissl et al, 2008;Stieglmeier et al, 2009;La Duc et al, 2014;Smith et al, 2022;Lu et al, 2023). Moraxellaceae dominated in a cleanroom study of floor wipes that focused on human pathogens (Bashir et al, 2016) and were dominant, along with actinobacteria, in a recent metagenomic study (Highlander et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

Succession of the bacterial community from a spacecraft assembly clean room when enriched in brines relevant to Mars

Carte,

Chen,

Clark

et al. 2023

International Journal of Astrobiology

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Interplanetary spacecraft are built in a spacecraft assembly facility (SAF), a clean room designed to reduce microbial contamination that could confound life detection missions or influence native ecosystems. The frigid hyperarid near-surface environment of Mars has ample hygroscopic Mg and Na salts of chloride, (per)chlorate and sulphate that may deliquesce to form dense brines, liquids with low water activity, and freezing points <0°C. The current study sought to define the climax microbial community after 6 mo of enrichment of SAF floor wipe samples in salt plains medium supplemented with 50% (w/v; ~2 M; aw = 0.94) MgSO4 or 20% (w/v; ~1.9 M; aw = 0.91) NaClO3. After 1 wk, 4 wk and 6 mo of incubation, metagenomic DNA extracts of the enriched SAF microbial community were used for high-throughput sequencing of 16S rRNA genes and subsequent phylogenetic analyses. Additionally, dozens of bacterial strains were isolated by repetitive streak-plating from the climax community after 6 mo of enrichment. Early in the enrichment, staphylococci greatly dominated and then remained abundant members of the community. However, actinobacteria succeeded the staphylococci as the dominant taxa as the cultures matured, including Arthrobacter, Brachybacterium and Brevibacterium. A diverse assemblage of bacilli was present, with Oceanobacillus being especially abundant. The SAF culture collection included representatives of Brachybacterium conglomeratum, Brevibacterium sediminis, Oceanobacillus picturae and Staphylococcus sciuri. These were characterized with biochemical and physiological tests, revealing their high salinotolerance. Shannon diversity indices were generally near 2, reflecting modest diversity at several levels of identity and the community structures were uneven throughout. However, minor members of the community seem capable of the ecosystem functions required for biogeochemical cycling. For instance, organisms capable of all the functions of the N cycle were detected. The microbial assemblage in SAFs is the most likely to be transported by spacecraft to another world. While individual microbial populations may exhibit the qualities needed for survival at the near-surface of Mars, certainly entire communities with the capacity for complete biogeochemical cycling, would have a greater chance of survival and proliferation.

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Alternations of antibiotic resistance genes and microbial community dynamics on shared bicycles before and after pandemic lockdown

Xiu,

Liu,

Xie

et al. 2024

Science of The Total Environment

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Characteristics of bacterial community and ARG profiles in the surface and air environments in a spacecraft assembly cleanroom

Cited by 3 publications

References 64 publications

Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Succession of the bacterial community from a spacecraft assembly clean room when enriched in brines relevant to Mars

Alternations of antibiotic resistance genes and microbial community dynamics on shared bicycles before and after pandemic lockdown

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