Characteristics of antibiotic sensitivity of Staphylococcus aureus isolated from dairy farms in Ukraine

Abstract

Staphylococcus aureus is one of the most important microorganism in the process of raw milk production and has significance for people’s health as it causes dangerous microbial contamination of dairy production. Furthermore, raw milk and the environment of livestock farms may be potential vehicles for distribution of antibiotic-resistant strains of S. aureus. The aim of the present study was to establish antibiotic sensitivity profiles of S. aureus depending on its origin from dairy farms, with a special focus on methicillin-resistant isolates. A total of 165 samples were collected for investigation in the period 2014–2016 from 5 dairy farms in Ukraine. All samples were analyzed for the presence of S. aureus using the Baird Parker Agar with Egg Yolk Tellurite Emulsion. Typical staphylococcal colonies were examined morphologically and for presence of coagulase and hemolysin activities. From these, positive samples for S. aureus were 62 (37.6%): 4 (6.5%) raw milk, 17 (77.4%) swabs of udder skin, 18 (29.0%) milk from cows with subclinical mastitis and 21 (33.9%) environmental samples. The standard disk diffusion method was used to determine sensitivity of S. aureus isolates to 10 antibiotics. The antimicrobial sensitivity profiles of S. aureus isolates showed differences between them, which depends on the origin of the isolates. Our results demonstrated that most of S. aureus isolates were resistant to penicillin, oxacillin and vancomycin. Of the 62 S.aureus isolates, 20 (32.3%) and 5 (8.1%) were found to be multiresistant to 3 different antibiotics, 6 (9.8%) isolates to 4 antibiotics, 12 (19.4%) and 3 (4.8%) to 5 antibiotics (P10, OX1, VA5, L10, TE30 and P10, OX1, VA5, CIP10, TE30 respectively). All isolates resistant to penicillin and oxacillin were typed by mec A gene in PCR with two primers (MecA147-F and MecA147-R). The results show that 66.7% of these isolates yielded a mecA product. The information obtained from this study is useful for understanding the prevalence of S. aureus and its antibiotic sensitivity in dairy farms and can be useful for local and national monitoring or for designing specific control programs of methicillin- and multiresistance isolates present in the food chain of milk production.

Antimicrobial Susceptibility of Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli Isolated from Mastitic Dairy Cattle in Ukraine

“…It is worth noting that whereas 16.95% of the S. aureus isolates were resistant to gentamicin in this study, much fewer resistant S. aureus isolates were identified by a previous Ukrainian study in which only 2 of 62 mastitis isolates tested resistant to this aminoglycoside [16]. In agreement with this earlier Ukrainian bovine mastitis study, in the current investigation, tetracycline effectively suppressed S. aureus growth in vitro [16]. In the present study, tetracycline was also found to be highly efficacious against E. coli isolates, whereas resistance to this antimicrobial was frequently observed among St. agalactiae, with 80% of the isolates demonstrating this resistance trait.…”

“…Gentamicin usage in food animals, however, is carefully regulated in the European Union, and the European Commission has set limits on the allowable gentamicin residues found in animal food products [25]. It is worth noting that whereas 16.95% of the S. aureus isolates were resistant to gentamicin in this study, much fewer resistant S. aureus isolates were identified by a previous Ukrainian study in which only 2 of 62 mastitis isolates tested resistant to this aminoglycoside [16]. In agreement with this earlier Ukrainian bovine mastitis study, in the current investigation, tetracycline effectively suppressed S. aureus growth in vitro [16].…”

“…Given that there are almost no peer-reviewed publications on antimicrobial resistance in animal husbandry of Ukraine [15][16][17], the aim of this study was to assess the antimicrobial susceptibility patterns of mastitis-causing pathogens, specifically, E. coli, S. aureus, and St. agalactiae isolated from dairy cattle with mastitis in Ukraine.…”

The activity of the disinfectant “Enzidez” against bacteria in biofilms

“…Для цього використовуються та поєднаються між собою дезінфікуючі субстанції із різних груп, дія яких направлена на пригнічення активності різних ферментних систем бактеріальної клітини та руйнування її структурних елементів (Augustin et al, 2004;Kovalenko et al, 2018). Проте, незважаючи на достатньо велику кількість дезінфікуючих засобів на ринку, ідеального -препарату не існує, так як мікроорганізми доволі швидко адаптуються до нових антибактеріальних субстанцій (Berhilevych et al, 2017;.…”

“…Наявність мікробних біоплівок на поверхнях медичних інструментів, операційному обладнанні, протезах, катетерах, технологічних лініях у харчовій промисловості вважається чинником, який сприяє інфікуванню макроорганізму, забрудненню сировини та продукції (Hoque et al, 2015;Kukhtyn et al, 2017;Berhilevych et al, 2017). Тому використання біоцидів направлене на знищення планктонних і біоплівкових форм мікроорганізмів на різних поверхнях (Augustin et al, 2004;Kovalenko et al, 2018;Palii et al, 2020).…”