Characteristics of an Established Cell Line of Retinoblastoma2

Reid, Ted W.; Albert, Daniel M.; Rabson, Alan S.; Russell, Paul; Craft, Joseph L.; Chu, Elizabeth W.; Tralka, Tommie Sue; Wilcox, Janice L.

doi:10.1093/jnci/53.2.347

Cited by 303 publications

(168 citation statements)

References 0 publications

Supporting

Mentioning

152

Contrasting

Unclassified

Order By: Relevance

“…Compared to the retinoblastoma cell lines available to date, these cells have a unique characteristic of growing in non-adherent spheres. the doubling time is approximately 60 h, which is longer than Y79 and sNuOt-rb1 cells (23,40). Figure 7.…”

Section: Discussionmentioning

confidence: 93%

Maintenance of retinal cancer stem cell-like properties through long-term serum-free culture from human retinoblastoma

Jiao

2011

Oncol Rep

View full text Add to dashboard Cite

Abstract. previous studies have demonstrated that a small population of cancer stem cell-like cells exists in retinoblastoma. to provide a model for studying this population, we sought to establish a long-term culture from human retinoblastoma that have cancer stem cell-like properties. Fresh tumor tissue was digested and cultured in serum-free medium. tumor spheres formed and were passaged continuously. stem cell properties were examined through immunostaining, real-time quantitative rt-pCr and chemoresistance assay. tumorigenicity of the tumor sphere-forming cells was confirmed by xenograft experiments. Furthermore, we examined the expression of cell surface markers CD44 and CD133. tumor cells expanded as floating spheres for more than 30 passages. Sphere-forming cells overexpressed stem cell genes Oct-4, Nestin and Pax6. Immunostaining of spheres showed positivity for Nestin, pax6 and also ABCG2. In contrast, differentiated cells derived from these spheres expressed high levels of mature retinal cell markers MAp2, GFAp, recoverin, Opsin B and Nrl, and showed immunoreactivity for NF200, GFAp, recoverin and pKCα. Furthermore, both CD44 and CD133 were highly expressed in sphere-forming cells vs. differentiated cells. sphere-forming cells displayed higher chemoresistance to carboplatin as opposed to differentiated cells. Moreover, intraocular injection of as few as 2x10 3 sphere-forming cells into NOD/sCID mice gave rise to new tumors similar to the original patient tumors. these results revealed that the sphere-forming cells preserved their stem cell properties and tumorigenicity, even after longterm culture. this would be a suitable in vitro model to study cancer stem-like cells in retinoblastoma and to develop chemotherapeutic drugs and strategies.

show abstract

Section: Discussionmentioning

confidence: 93%

Maintenance of retinal cancer stem cell-like properties through long-term serum-free culture from human retinoblastoma

Jiao

2011

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…The average population-doubling time of SNUOT-Rb1 was 24 h, whereas that of Y79 was 33 h in suspension or 50 h in attached monolayer (Fig. 2C) (8,9).…”

Section: Morphological and Growth Characteristics Of Snuot-rb1mentioning

confidence: 95%

“…For research on cancer biology, tumor cell culture is an effective tool to identify the cell of origin as well as subsequent genetic defects that contribute to pathogenesis. The majority of in vitro and in vivo investigations for retinoblastoma have been carried out using human retinoblastoma cell lines such as Y79 (8) and WERI-Rb1 (9), which both spontaneously grow in suspension. Here we report the establishment and characterization of a new, spontaneously immortalized human retinoblastoma cell line, designated SNUOT-Rb1.…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a novel, spontaneously immortalized retinoblastoma cell line with adherent growth

Kim

Kim²,

Yu³

et al. 2007

Int J Oncol

View full text Add to dashboard Cite

Abstract. Retinoblastoma is the most common intraocular cancer of childhood, however, only a few cultured retinoblastoma cell lines are available to date. In the present study, we established a new human retinoblastoma cell line with adherent growth, named SNUOT-Rb1. The SNUOT-Rb1 cell line was established from an eye with retinoblastoma, which was enucleated from a 3-year-old Korean child. SNUOT-Rb1 has morphological and biochemical characteristics common to previous human retinoblastoma cell line, Y79: morphological features of fibroblast-or ganglion-like cells, and biochemical features of expression of glial fibrillary acidic protein and neuron-specific enolase. However, compared to Y79, SNUOTRb1 has a unique characteristic of growing in adherence, and the doubling time of SNUOT-Rb1 is shorter than Y79 in adherent or floating growth. In analysis of the tumorigenic potential of SNUOT-Rb1 in nude mice, orthotopic implantation of SNUOT-Rb1 mimics the pattern of local growth of retinoblastoma. In comparative genomic hybridization analysis, we found that SNUOT-Rb1 has significant chromosomal imbalances on chromosome 3, 9, 10, 11, 14, 16, 17, and 22. Therefore, SNUOT-Rb1 could be useful in studying the biological and genetic characteristics of retinoblastoma for insights into the heredity and genetics of childhood cancer.

show abstract

“…Y-79 retinoblastoma cells were cultured as described by Reid et al (1974). Cells were maintained in RPMI 1640 medium containing 10% fetal calf serum, 2 mM glutamine, 50 units/mi penicillin, and 50 jig/mi streptomycin at 37°C, 95% humidity, and 8% CO 2.…”

Section: Tissue Culturementioning

confidence: 99%

Modulation of Human Glutamate Transporter Activity by Phorbol Ester

Ganel¹,

Crosson

1998

Journal of Neurochemistry

View full text Add to dashboard Cite

Termination of synaptic glutamate transmission depends on rapid removal of glutamate by neuronal and glial high-affinity transporters. Molecular biological and pharmacological studies have demonstrated that at least five subtypes of Na~-dependent mammalian glutamate transporters exist. Our study demonstrates that Y-79 human retinoblastoma cells express a single Na~-dependent glutamate uptake system with a Km of 1.7 ±0.42 1iM that is inhibited by dihydrokainate and DLthreo-~3-hydroxyaspartate (IC50 = 0.29 ±0.17~M and 2.0 ±0.43 btM, respectively). The protein kinase C activator phorbol 1 2-myristate 13-acetate caused a concentrationdependent inhibition of glutamate uptake (1050 = 0.56 ±0.05 nM), but did not affect Na~-dependent glycine uptake significantly. This inhibition of glutamate uptake resulted from a fivefold decrease in the transporter's affinity for glutamate, without significantly altering the Vmax. 4a-Phorbol 12,13-didecanoate, a phorbol ester that does not activate protein kinase C, did not alter glutamate uptake significantly. The phorbol 1 2-myristate 13-acetateinduced inhibition of glutamate uptake was reversed by preincubation with staurosporine. The biophysical and pharmacological profile of the human glutamate transporter expressed by the Y-79 cell line indicates that it belongs to the dihydrokainate-sensitive EAAT2/GLT-1 subtype. This conclusion was confirmed by western blot analysis. Protein kinase C modulation of glutamate transporter activity may represent a mechanism to modulate extracellular glutamate and shape postsynaptic responses. Key Words: Glutamate-Transporter-Phorbol ester-Protein kinase C-Kinetics-Y-79 cells.

show abstract

Characteristics of an Established Cell Line of Retinoblastoma2

Cited by 303 publications

References 0 publications

Maintenance of retinal cancer stem cell-like properties through long-term serum-free culture from human retinoblastoma

Maintenance of retinal cancer stem cell-like properties through long-term serum-free culture from human retinoblastoma

Establishment and characterization of a novel, spontaneously immortalized retinoblastoma cell line with adherent growth

Modulation of Human Glutamate Transporter Activity by Phorbol Ester

Contact Info

Product

Resources

About