Characteristics of an ammonia-oxidizing bacterium with a plasmid isolated from alkaline soils and its phylogenetic relationship

Takahashi, Reiji; OHISHI, MOTOKI; Ohshima, Mifuyu; Saitoh, Masami; Omata, Kana; Tokuyama, Tatsuaki

doi:10.1016/s1389-1723(01)80255-3

Cited by 13 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The purification of the strain SW16 was conducted using three types of heterotrophic mediums. The identification process of the strain SW16, including DNA extraction, PCR amplification, the sequencing of amplified 16S rRNA genes and data analysis for construction of a phylogenetic tree, was performed in the previous report (Takahashi et al 2001).…”

Section: Aob Strain and Preparation Of Inoculamentioning

confidence: 99%

Ammonia adsorption and nitritation in sediments derived from the Three Gorges Reservoir, China

et al. 2009

View full text Add to dashboard Cite

The influences of suspended particles (SPs) on NH 4? adsorption and nitritation occurring in the water system of the Three Gorges Reservoir (TGR) were evaluated in this study. The results indicated that the adsorption of NH 4 ? was significantly affected by the SPs concentration under the conditions typically present in the TGR. The amount of ammonia adsorbed per unit weight of suspended particles was inverse proportional to the concentration of suspended particles. However, the influences of the particle size and the organic matter concentration existing in SPs were insignificant under the experimental conditions. The effects of suspended particles on nitritation were determined by the use of ammonia-oxidizing bacteria (AOB) strain SW16, identified as Nitrosomonas nitrosa, which was isolated from sediment samples of the TGR. Suspended particle concentration in water-sediment solution played an important role in the nitritation process. The rate of nitritation enhanced with the increase of the suspended particle concentration. It was found that the critical factor controlling ammonia oxidizing rate was the AOB biomass resulting from the AOB growth rate. Moreover, results demonstrated that both particle size and organic matter content showed little effect on the nitritation process under the experimental conditions.

show abstract

Section: Aob Strain and Preparation Of Inoculamentioning

confidence: 99%

Ammonia adsorption and nitritation in sediments derived from the Three Gorges Reservoir, China

et al. 2009

View full text Add to dashboard Cite

show abstract

“…2004, 2005; Potter and Fothergill‐Gilmore 1991; Satoh et al. 2003; Takahashi et al. 2001; Tomiyama et al.…”

Section: Methodsmentioning

confidence: 99%

“…Simultaneously, we supplied evidence that supported our previous results related to the use of housekeeping genes. Table 1 lists the 16 AOB used in the present study, their abbreviations and the origins of their pure cultures (Ida et al 2004(Ida et al , 2005Potter and Fothergill-Gilmore 1991;Satoh et al 2003;Takahashi et al 2001;Tomiyama et al 2001). The AOB were cultured using HEPES medium under conditions previously described (Satoh et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Classification of chemoautotrophic ammonia-oxidizing bacteria using pyruvate kinase genes as high resolution phylogenetic markers

Ida

Takahashi

Satoh

et al. 2006

Soil Science and Plant Nutrition

Self Cite

View full text Add to dashboard Cite

The phylogenetic relationship of β-proteobacterial ammonia-oxidizing bacteria (AOB) is mainly based on comparative sequence analyses of the genes encoding the 16S rRNA (16S rDNA) and the active site polypeptide of the ammonia monooxygenase (AmoA). However, classification of AOB with different morphotypes is not possible based solely on these markers. Pyruvate kinase (PYK), which is a key enzyme in the glycolytic pathway, functions at a primary metabolic intersection. The gene sequence of PYK is known to be a phylogenetic marker for bacteria and has been used for this purpose. The phylogenetic relationships of closely related AOB, 'Nitrosolobus', 'Nitrosovibrio' and Nitrosospira, were estimated based on pyk (PYK), a housekeeping and protein-coding gene. As a result, the pyk phylogenetic tree of the AOB showed a high resolution compared with the tree based on 16S rDNA sequences. Therefore, we conclude that analysis of the PYK gene sequences could play an important role in the in-depth classification and identification of AOB at a rank below the genus level.

show abstract

Biological Effects

Shen¹,

Niu²,

Wang³

et al. 2013

Advanced Topics in Science and Technology in China

View full text Add to dashboard Cite

Characteristics of an ammonia-oxidizing bacterium with a plasmid isolated from alkaline soils and its phylogenetic relationship

Cited by 13 publications

References 21 publications

Ammonia adsorption and nitritation in sediments derived from the Three Gorges Reservoir, China

Ammonia adsorption and nitritation in sediments derived from the Three Gorges Reservoir, China

Classification of chemoautotrophic ammonia-oxidizing bacteria using pyruvate kinase genes as high resolution phylogenetic markers

Biological Effects

Contact Info

Product

Resources

About