Characteristics of airborne bacterial communities and antibiotic resistance genes under different air quality levels

Xu, Yan; Ma, Jiahui; Chen, Xinqing; Miao, Lei; Li, Tianning; Han, Yizhuo

doi:10.1016/j.envint.2022.107127

Cited by 27 publications

(12 citation statements)

References 76 publications

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“…In the current study, SO 4 2– , NO 3 – , and temperature were all identified as significant influencers of the bacterial community structure, which indirectly altered the airborne resistome (Figure a,b,d). Indeed, SO 4 2– and NO 3 – are essential nutrients involved in metabolism, while temperature is the main driver of bacterial activity, both factors can shape the bacterial community structure by affecting microbial growth and interactions. , It should be noted that the HGT-mediated driving force was not considered in this study. On the one hand, the strong linkage between PM 2.5 -borne resistome and bacterial communities indicates that the HGT of ARGs may not be sufficiently frequent to hide their association with bacterial genomes, especially considering that only a very limited (<1%) ARGs co-occurred with MGEs on the same contig in intracellular DNA.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Zhang,

Wang,

et al. 2023

Environ. Sci. Technol.

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Bioaerosol pollution poses a substantial threat to human health during municipal food waste (FW) recycling. However, bioaerosol-borne antibiotic-resistant genes (ARGs) have received little attention. Herein, 48 metagenomic data were applied to study the prevalence of PM2.5-borne ARGs in and around full-scale food waste treatment plants (FWTPs). Overall, FWTP PM2.5 (2.82 ± 1.47 copies/16S rRNA gene) harbored comparable total abundance of ARGs to that of municipal wastewater treatment plant PM2.5 (WWTP), but was significantly enriched with the multidrug type (e.g., AdeC/I/J; p < 0.05), especially the abundant multidrug ARGs could serve as effective indicators to define resistome profiles of FWTPs (Random Forest accuracy >92%). FWTP PM2.5 exhibited a decreasing enrichment of total ARGs along the FWTP–downwind–boundary gradient, eventually reaching levels comparable to urban PM2.5 (1.46 ± 0.21 copies/16S rRNA gene, N = 12). The combined analysis of source-tracking, metagenome-assembled genomes (MAGs), and culture-based testing provides strong evidence that Acinetobacter johnsonii-dominated pathogens contributed significantly to shaping and disseminating multidrug ARGs, while abiotic factors (i.e., SO4 2–) indirectly participated in these processes, which deserves more attention in developing strategies to mitigate airborne ARGs. In addition, the exposure level of FWTP PM2.5-borne resistant pathogens was about 5–11 times higher than those in urban PM2.5, and could be more severe than hospital PM2.5 in certain scenarios (<41.53%). This work highlights the importance of FWTP in disseminating airborne multidrug ARGs and the need for re-evaluating the air pollution induced by municipal FWTP in public health terms.

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Section: Discussionmentioning

confidence: 99%

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Zhang,

Wang,

et al. 2023

Environ. Sci. Technol.

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“…tetW concentrations started to decrease at low/moderate levels of pollution, showing how different ARGs may respond differently to varying pollution levels. 42 Atmospheric transport from different regions could also play a role, as it has been shown to contribute 15–55% of PM 2.5 levels during different haze events in Beijing. 43 Shifts in relative abundance profiles of individual ARGs while in the air could also lead to variability throughout the day; however, these mechanisms are still not understood.…”

Section: Comparing Airborne Arg Profiles Reported Across Studiesmentioning

confidence: 99%

Critical review of antibiotic resistance genes in the atmosphere

Kormos

Lin

Pruden

et al. 2022

Environ. Sci.: Processes Impacts

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“…Bioparticle is a kind of particulate matter consisting of fungal spores, bacteria, and viruses in fine and ultrafine particles 5,6) . Bioparticles can easily be dispersed in ambient air as atmospheric particles, airborne particles, or bioaerosol.…”

Section: Introductionmentioning

confidence: 99%

“…Bioparticles can easily be dispersed in ambient air as atmospheric particles, airborne particles, or bioaerosol. They can be found in indoor and outdoor areas, such as medical rooms, isolation rooms, small rooms, and even house 5,7,8) . Bacteriophage and SARS-CoV-2 (coronavirus) 9,10) are examples of bioparticles in terms of virus type.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide-Coated Quartz Crystal Microbalance for Bioparticle Detection (A Case Study for Bacillus sp.)

Budianto¹,

Wardoyo²,

Masruroh³

et al. 2023

Evergreen

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Bioparticle concentration can be measured using many techniques, such as mass spectroscopy, light scattering, and fluorescent tracking. Due to some instrument limitations, high cost, low sensitivity-selectivity level, and performance development needs, there is a need to develop a new sensing technique with higher performance. This study used quartz crystal microbalance (QCM) and graphene oxide (GO) as the selective coating materials. For this purpose, two different sensors were tested: uncoated QCM and GO-coated QCM. The sensor performances were tested inside an exposure chamber by exposing the sensors to different bacteria samples: B. pasteurii, B. sphaericus, and B. cereus. The sensor's frequency shift was counted using a frequency counter, while the sample concentration was measured using a Digital Dust Monitor. The results show that the sensors work well in detecting Bacillus sp. samples with sensitivities of 0.24 to 0.35 Hz.µg -1 . The developed sensors have a response time of <96 s, with a linearity of 95%. The best response is obtained from the coated QCM using B. pasteurii. The coated QCM performs better than the uncoated QCM, indicated by better linearity, response time, and sensitivity.

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Characteristics of airborne bacterial communities and antibiotic resistance genes under different air quality levels

Cited by 27 publications

References 76 publications

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Critical review of antibiotic resistance genes in the atmosphere

Graphene Oxide-Coated Quartz Crystal Microbalance for Bioparticle Detection (A Case Study for Bacillus sp.)

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Characteristics of airborne bacterial communities and antibiotic resistance genes under different air quality levels

Cited by 27 publications

References 76 publications

Pathogenic Bacteria Are the Primary Determinants Shaping PM2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Pathogenic Bacteria Are the Primary Determinants Shaping PM2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Critical review of antibiotic resistance genes in the atmosphere

Graphene Oxide-Coated Quartz Crystal Microbalance for Bioparticle Detection (A Case Study for Bacillus sp.)

Contact Info

Product

Resources

About

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System