Characteristics of a novel low density cell-immobilized magnetic supports in liquid magnetically stabilized beds

Al-Qodah, Zakaria; Al-Shannag, Mohammad; Assirey, Eman; Orfali, Wasim; Bani‐Melhem, Khalid; Alananbeh, Kholoud M.; Bouqellah, Nahla A.

doi:10.1016/j.bej.2015.01.004

Cited by 14 publications

(5 citation statements)

References 32 publications

(31 reference statements)

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“…The Langmuir, Freundlich and Hill models were applied in order to assess the mechanism of adsorption . Table shows the experimental maximum dispersion limit of protein according to the isotherms of adsorption and the fitting of the experimental data to the adsorption models.…”

Section: Resultsmentioning

confidence: 99%

“…The Langmuir, Freundlich and Hill models were applied in order to assess the mechanism of adsorption. 27 -29 Table 1 shows the experimental maximum dispersion limit of protein according to the isotherms of adsorption and the fitting of the experimental data to the adsorption models. In addition, the theoretical maximum dispersion limit (Q MAX and K F ) according to the three adsorption models, the coefficient of determination R 2 , the residual sum of squares Σ 2 ; and the various constants derived from the models are presented.…”

Section: Resultsmentioning

confidence: 99%

“…The fitting of the adsorption equilibrium data (isotherm of adsorption) to the Langmuir, Freundlich and Hill models was investigated . The inverse of the amount of protein adsorbed (C ads , µmol m −2 ) versus the inverse of the concentration of protein in contact with the TiO 2 NPs at equilibrium ( C eq , mg mL −1 ) was represented according to the linear form of the Langmuir model.…”

Section: Methodsmentioning

confidence: 99%

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Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO₂ nanoparticles for improved biocatalytic performance

Suster

Toledo

Fittipaldi

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND The immobilization of the lipase B of Candida antarctica CALB over TiO2 nanoparticles was thoroughly investigated with the isotherms of adsorption at various temperatures with and without the addition of sorbitol and glycerol. The surface composition, secondary structure and the effect of the addition of the polyols was addressed. RESULTS The maximum dispersion limit of protein on TiO2 nanoparticles (NPs) is 0.073 ± 0.007 µmol m−2. Glycerol and sorbitol co‐adsorb on the TiO2 NPs reaching 45% of the surface composition of the biocatalyst. The optimized material was able to catalyze the esterification of 52% of R/S‐ibuprofen with ethanol (0.31 ± 0.01 µmol min−1 mg−1) with 41% of enantiomeric excess towards S(+)‐ibuprofen in 24 h reaction. Under similar reaction conditions, the commercial counterpart Novozym® 435 showed 34% conversion (0.091 ± 0.003 µmol min−1 mg−1) and 16% of enantiomeric excess. CONCLUSIONS The molecular association between the protein and the polyols exerts a positive cooperativism which prevents aggregation of the protein and protects its active conformation. The residual esterase activity of the immobilized CALB compared with the free lipase depends directly on the amount of co‐adsorbed polyols. Moreover, polyols boost the catalytic performance in the kinetic resolution of racemic ibuprofen showing an optimum at the maximum coverage of polyols on the biocatalysts. © 2017 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO₂ nanoparticles for improved biocatalytic performance

Suster

Toledo

Fittipaldi

et al. 2017

J of Chemical Tech & Biotech

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“…For immobilized cells/enzymes, the magnetic eld leads to the enhancement of mass transfer, increased substrate utilization and product formation. 18,19 M-I-CALB was obtained by treating I-CALB with magnetic elds of 100, 300, or 500 mT for 1, 2, or 3 hours. M-I-CALB was subsequently used to catalyze the esteri-cation of naringin with palmitic acid.…”

Section: Effects Of M-i-calb On Initial Rate and Conversion Yields Amentioning

confidence: 99%

Effects of magnetic fields on the enzymatic synthesis of naringin palmitate

Zhang

Wan

et al. 2018

RSC Adv.

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“…5,6 Bioclogging has been the subject of many numerical and experimental studies which involved in a variety of applications such as biobarrier or biofouling. 7,8 Of course, bioclogging can also be optimized and improved through various processes, such as the application of magnetic stabilizing fillers 9 and magnetic immobilization techniques. 10,11 Bioclogging may reduce the efficiency of bioremediation because contaminated parts of an aquifer can clog due to microbial activity and rates of degradation can decrease.…”

Section: Introductionmentioning

confidence: 99%

Bioclogging effects on mass transport using volume averaging method in bioretention cell treating wastewater: Theories

Li,

Wang,

Jin

2024

Env Prog and Sustain Energy

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In bioretention cell (BRC) systems, internal clogging in the hydraulic field caused by microbial organisms growing in the nonuniform porous media can lead to the reduction of denitrification capacity. To investigate microbial effect processes, a multispecies biological field‐hydraulic coupling transport model in BRC was developed based on the average volume method (AVM) and traditional convection‐diffusion–reaction equation. This model provides a method to establish a connection between the microscale biofilm thickness and the macroscale measurement of pollutant concentration, which has been less frequently discussed in previous research. The present work differs from earlier works by explicitly smoothing the diversity and complexity of media structure by upscaling the physical quantities using the AVM. The results suggest that the model is derived for enhancing a more comprehensive understanding the relationship between the microscopic biofilm thickness and the measured concentration. It is also found that the infiltration description is required adding porosity parameter in order to have an accurate simulation of permeable phenomena under biologically growing condition.

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Characteristics of a novel low density cell-immobilized magnetic supports in liquid magnetically stabilized beds

Cited by 14 publications

References 32 publications

Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO₂ nanoparticles for improved biocatalytic performance

Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO₂ nanoparticles for improved biocatalytic performance

Effects of magnetic fields on the enzymatic synthesis of naringin palmitate

Bioclogging effects on mass transport using volume averaging method in bioretention cell treating wastewater: Theories

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Characteristics of a novel low density cell-immobilized magnetic supports in liquid magnetically stabilized beds

Cited by 14 publications

References 32 publications

Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO2 nanoparticles for improved biocatalytic performance

Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO2 nanoparticles for improved biocatalytic performance

Effects of magnetic fields on the enzymatic synthesis of naringin palmitate

Bioclogging effects on mass transport using volume averaging method in bioretention cell treating wastewater: Theories

Contact Info

Product

Resources

About

Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO₂ nanoparticles for improved biocatalytic performance

Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO₂ nanoparticles for improved biocatalytic performance