Characteristics of a birnavirus isolated from cultured sand goby Oxyeleotris marmoratus

Hedrick, RP; Eaton, William D.; Fryer, J.L.; Groberg, WG; Boonyaratapalin, S

doi:10.3354/dao001219

Cited by 33 publications

(17 citation statements)

References 11 publications

(11 reference statements)

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“…reaction sites or multiple d~screte antigens. On the other hand, Hedrick et al (1986) found that comparisons of the virion polypeptides and RNA segments of new isolates are helpful in determining the ident~ty of individual virus strains. In addition, MacDonald & Gower (1981) reported that the study of the patterns of RNA might show more diversity than serological methods.…”

Section: Pathogenicity Testsmentioning

confidence: 99%

Characterization of a birnavirus isolated from diseased turbot cultured in Spain

Novoa¹,

Figueras²,

Puentes³

et al. 1993

Dis. Aquat. Org.

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During 1989, light but persistent mortalities were detected in a turbot Scophthalnius maximus L. farm in Galicia (northwestern Spaln) and a virus with the characteristics of a blrnavirus was isolated. The purpose of this study was to characterize the viral agent and determine the susceptibility of turbot to this virus. Electron microscopic examination revealed that the particles were so metric, hexagonal and unenveloped with an average dlameter of 58 to 60 nm The molecular weights of the RNA segments were 1.9 and 2.0 X l o b daltons. The cells most susceptible to the turbot isolate were the CHSE-214, FHM and RTG-2 lines and the optimal temperature range for its replication was 15 to 2OoC. The RNA and polypeptide electropherotypes show that thls virus resembles the Ab serotype of infechous pancreahc necrosis vuus (IPNV); however, it dlffers in that it replicates in the FHM cell llne and is not neutralized by antisera to the classical serotypes of IPNV. lnfectiv~ty tnals conducted in turbot of different sizes indicated that the virus produced mortality only in small fish (2 g ) , although the larger fish (30 g ) harbored the virus for at least 35 d . Fish inoculated with this isolate showed no pancreatic necrosis although necrosis of the hematopoietic elements of the ludney and spleen was detected.

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Section: Pathogenicity Testsmentioning

confidence: 99%

Characterization of a birnavirus isolated from diseased turbot cultured in Spain

Novoa¹,

Figueras²,

Puentes³

et al. 1993

Dis. Aquat. Org.

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“…Various organisms (viruses, bacteria, fungi and higher parasites) were found to be associated with the diseased fish (Hedrick et al 1986, Tonguthai 1986, Wattanavijarn et al 1986, Boonyaratpalin 1989, Frerichs et al 1989. Six rhabdovirus isolations have been reported from snakehead fish with ulcerative disease; however, there is no known causal relationship.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of three rhabdoviruses from snake-head fish Ophicephalus striatus

Kasornchandra¹,

Engelking²,

Lannan³

et al. 1992

Dis. Aquat. Org.

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Protein profiles and serological characteristics of 3 rhabdoviruses from snakehead fish Ophicephalus striatus were determined and compared to 5 known fish rhabdoviruses and 1 mammalian rhabdovirus. The snakehead rhabdovirus (SHRV) exhibited a bacilliform morphology and a Lyssavirus-type protein profile. The ulcerative disease rhabdovirus isolates (UDRV-BP and UDRV-19) were indistinguishable and exhibited bullet-shaped morphology and a Vesiculovirus-type protein profile. At present, none of the 3 viruses is known to be the cause of disease in any species of fish. UDRV-BP and UDRV-19 were serologically identical but distinct from SHRV and from 5 other fish rhabdoviruses. SHRV was serologically unrelated to any of the fish rhabdoviruses examined.

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“…The disease was characterized by a severe ulcerative dermal necrosis and was seen in a wide variety of freshwater and estuarine fishes, in the wild and in pond culture, throughout Southeast Asia. Although the disease affected several species, the cultured snakehead fish, the walking catfish (Clarius batrachus), and the sand goby (Oxyeleotris marmoratus) were the most noticeably affected (10). SHRV is a nonsegmented negative-strand RNA virus from the Rhabdoviridae family, genus Novirhabdovirus.…”

mentioning

confidence: 99%

The NV Gene of Snakehead Rhabdovirus (SHRV) Is Not Required for Pathogenesis, and a Heterologous Glycoprotein Can Be Incorporated into the SHRV Envelope

Alonso

Kim

Johnson

et al. 2004

J Virol

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Snakehead rhabdovirus (SHRV) affects warm-water fish in Southeast Asia and belongs to the genus Novirhabdovirus by virtue of its "nonvirion" (NV) gene. To examine the function of the NV gene, we used a recently developed reverse genetic system to produce a viable recombinant SHRV carrying an NV gene deletion. The recombinant virus was produced at the same rate and same final concentrations as the wild-type virus in cultured fish cells in spite of the NV gene deletion. The role of the NV protein in fish pathogenesis was also investigated. Zebra fish (Danio rerio) were infected with the NV deletion mutant or with a recombinant virus containing a copy of the SHRV genome, and similar mortality rates as well as final mortalities were recorded, suggesting no apparent role for the NV protein in fish pathogenesis. Interestingly, the unsuccessful rescue of fully viable recombinants with genomes containing deletions in the G/NV gene junction suggested a role for the gene junction in virus transcription and replication. Finally, we demonstrated that the SHRV glycoprotein can be replaced by the glycoprotein of infectious hematopoietic necrosis virus (IHNV) or by a hybrid protein composed of SHRV and IHNV sequences.Snakehead rhabdovirus (SHRV), a rhabdovirus of warmwater fish, was isolated from a diseased snakehead fish (Ophicephalus striatus) during an epizootic outbreak in Thailand (12). The disease was characterized by a severe ulcerative dermal necrosis and was seen in a wide variety of freshwater and estuarine fishes, in the wild and in pond culture, throughout Southeast Asia. Although the disease affected several species, the cultured snakehead fish, the walking catfish (Clarius batrachus), and the sand goby (Oxyeleotris marmoratus) were the most noticeably affected (10). SHRV is a nonsegmented negative-strand RNA virus from the Rhabdoviridae family, genus Novirhabdovirus. The genome of SHRV contains six open reading frames (ORF) in the order 3Ј-N-P-M-G-NV-L-5Ј (13). At the junction between two genes, the stop/polyadenylation signal and the following start signal are separated by an intergenic region composed of several nucleotides that are not present in the mRNAs. The major distinguishing feature of the Novirhabdoviruses is the presence of a nonvirion (NV) gene between the viral glycoprotein (G) and the polymerase (L) genes (13). The NV gene contains a single ORF, which is 335 nucleotides long. The NV protein has been detected in infectious hematopoietic necrosis virus (IHNV)-infected tissue culture cells by autoradiography (14) and immunofluorescence techniques (21) and in tissues of IHNV-infected fish by confocal microscopy (6). While the functions of most IHNV proteins have been determined, the function of the NV protein remains unknown.In 2000, we reported the recovery of recombinant snakehead rhabdovirus (SHRV) from a full-length cDNA clone of the viral genome (11). The full-length cDNA was assembled from clones of each of the five SHRV genes and their intergenic regions and inserted into a transcription plasmid...

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Characteristics of a birnavirus isolated from cultured sand goby Oxyeleotris marmoratus

Cited by 33 publications

References 11 publications

Characterization of a birnavirus isolated from diseased turbot cultured in Spain

Characterization of a birnavirus isolated from diseased turbot cultured in Spain

Characteristics of three rhabdoviruses from snake-head fish Ophicephalus striatus

The NV Gene of Snakehead Rhabdovirus (SHRV) Is Not Required for Pathogenesis, and a Heterologous Glycoprotein Can Be Incorporated into the SHRV Envelope

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