Characteristics of a Binding Protein-Dependent Transport System for
 sn
 -Glycerol-3-Phosphate in
 Escherichia coli
 That Is Part of the
 pho
 Regulon

Schweizer, Herbert P.; Argast, M; Boos, Winfried

doi:10.1128/jb.150.3.1154-1163.1982

Cited by 54 publications

(22 citation statements)

References 20 publications

(20 reference statements)

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“…GlyP was selected as a source of P instead of inorganic phosphate, which can effectively complex with Fe(III) into insoluble precipitates [ Francis and Dodge , ]. GlyP is one of the few organophosphates that can be transported across the cell membrane, forming a source of P but not serving a carbon substrate [ Schweizer et al , ]. An isolated identical bacterial community indigenous to Lake Valkea‐Kotinen was used as a bacterial inoculum.…”

Section: Methodsmentioning

confidence: 99%

The effect of iron on the biodegradation of natural dissolved organic matter

Xiao

Hoikkala²,

Kasurinen

et al. 2016

JGR Biogeosciences

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Iron (Fe) may alter the biodegradation of dissolved organic matter (DOM), by interacting with DOM, phosphorus (P), and microbes. We isolated DOM and a bacterial community from boreal lake water and examined bacterial growth on DOM in laboratory experiments. Fe was introduced either together with DOM (DOM‐Fe) or into bacterial suspension, which led to the formation of insoluble Fe precipitates on bacterial surfaces (Fe coating). In the latter case, the density of planktonic bacteria was an order of magnitude lower than that in the corresponding treatment without introduced Fe. The association of Fe with DOM decreased bacterial growth, respiration, and growth efficiency compared with DOM alone at the ambient concentration of dissolved P (0.16 µmol L−1), indicating that DOM‐associated Fe limited the bioavailability of P. Under a high concentration (21 µmol L−1) of P, bacterial biomass and respiration were similar or several times higher in the treatment where DOM was associated with Fe than in a corresponding treatment without Fe. Based on the next generation sequencing of 16S rRNA genes, Caulobacter dominated bacterial communities grown on DOM‐Fe. This study demonstrated that association of Fe with a bacterial surface or P reduces bacterial growth and the consumption of DOM. In contrast, DOM‐Fe is bioavailable and bound Fe can even stimulate bacterial growth on DOM when P is not limiting.

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Section: Methodsmentioning

confidence: 99%

The effect of iron on the biodegradation of natural dissolved organic matter

Xiao

Hoikkala²,

Kasurinen

et al. 2016

JGR Biogeosciences

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“…Two clones (B5 and D4) have insertions in genes coding for proteins similar to ABC transporter that have not been described previously in Brucella. In mutant D4, the transposon is inserted between two open reading frames (ORFs) that are highly homologous to ugpA and ugpB, which encode the ATP-binding protein and the permease, respectively, of an ABC transporter involved in the uptake of sn-glycerol-3-phosphate in E. coli (Schweizer et al, 1982). In mutant B5, the transposon is inserted in a gene homologous to rbsC-1, which encodes a permease of an uncharacterized sugar ABC transporter of the archea Archaeoglobus fulgidus.…”

Section: Molecular Analysis Of the Mutantsmentioning

confidence: 99%

Identification and characterization of in vivo attenuated mutants of Brucella melitensis

Lestrate

Delrue

Danese

et al. 2000

Molecular Microbiology

148

123

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SummaryBrucella melitensis 16M is a Gram-negative a 2 -proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within both professional and non-professional phagocytes. Signature-tagged mutagenesis (STM) was used to identify genes required for the in vivo pathogenesis of Brucella. A library of transposon mutants was screened in a murine infection model. Out of 672 mutants screened, 20 were not recovered after a 5 day passage in BALB/c mice. The attenuation of 18 mutants was confirmed using an in vivo competition assay against the wild-type strain. The 18 mutants were characterized further for their ability to replicate in murine macrophages and in HeLa cells. The sequences disrupted by the transposon in the mutants have homology to genes coding for proteins of different functional classes: transport, amino acid and DNA metabolism, transcriptional regulation, peptidoglycan synthesis, a chaperone-like protein and proteins of unknown function. The mutants selected in this study provide new insights into the molecular basis of Brucella virulence.

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“…The observation that mutations in phoB as well as double mutants in phoR and phoM prevent expression, identify the U gp transport system as a member of the pho regulon [4,21]. The apparent K m of uptake of Ugp for its major substrate G3P is around 2 ILM, thus the Ugp system has about a lO-fold higher affinity for G3P than the GlpT system [18]. Also, the range of substrate specificity is different with both systems.…”

Section: Substrate Specificity and Regulationmentioning

confidence: 99%

“…The substrate specificity of U gp is determined by a periplasmic binding protein [3] and membrane vesicles are, in contrast to GlpT-mediated transport [1], not active in Ugp-mediated transport [18].…”

Section: Substrate Specificity and Regulationmentioning

confidence: 99%

“…However, G3P can be used as precursor for phospholipid biosynthesis when an alternate carbon source is present and its carbon content can be incorporated into protein. This curious phenomenon is not understood [18]. Possibly, accumulating inorganic phosphate that cannot exit the cell exerts a feedback inhibition on the activity of the Ugp transport system.…”

Section: Introductionmentioning

confidence: 99%

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