Characteristics investigation on biofilm formation and biodegradation activities of Pseudomonas aeruginosa strain ISJ14 colonizing low density polyethylene (LDPE) surface

Abstract

The accelerated population and industrial development have caused an extensive increase in the use of plastic products. Since polyethylene degrades slowly generating poisonous compounds, therefore, elimination of plastic from the environment is the prerequisite requirement today. Biodegradation of plastics seems to be a convenient and effective method to curb this problem. In view of this, the present study focuses on LDPE degradation capability of bacterial strain Pseudomonas aeruginosa ISJ14 (Accession No. MG554742) isolated from waste dump sites. Further, the stability of 16S rDNA of the isolate was determined by applying bioinformatics tools. For biodegradation studies, the polyethylene films were incubated with the culture of P. aeruginosa ISJ14 in two different growth medium namely Bushnell Hass broth (BHM) and Minimal Salt medium (MSM) for 60 days at 37 °C on 180 rpm. In addition, hydrophobicity and viability of bacterial isolate along with quantification of total protein content was also done. The microbial degradation was confirmed by surface modification and formation of fissures on polyethylene surface along with the variation in the intensity of functional groups as well as an increase in the carbonyl index using field emission scanning electron microscopy (Fe-SEM) and Fourier transform infrared spectrophotometry (FTIR). These results indicate that P. aeruginosa strain ISJ14 can prove to be a suitable candidate for LDPE waste treatment without causing any harm to our health or environment.

Spectroscopic and chromatographic identification of bioprospecting bioactive compounds from cow feces: Antimicrobial and antioxidant activities evaluation of gut bacterium Pseudomonas aeruginosa KD155

“…The concept of studying free energy associated with the folding of 16S rDNA gene sequence might provide preliminary information to make a concurrent prediction on stabilities of the genes. In our study, P. aeruginosa KD155 showed −480.10 kcal/mol free energy of secondary structure which is more or less in accordance with the previous studies [ 50 , 51 ].…”

Biodegradation of Polypropylene By Pseudomonas Aeruginosaisolated From Wastewater Associated Soil; A Potential Method To Eliminate The Plastic Pollution To Save Ecosystem

“…The bacterium P.aeruginosa isolated from Sisdol land ll site and Sanothimi household garbge site soil in Nepal has similar biochemical properties(Badahitet al, 2018). It has been reported that P. aeruginosa ISJ14 showed tremendous e ciency to degrade low density polyethylene (LDPE) in BHM (Gupta and Devi, 2020).In contrast, the present study reports for the rst time that P.aeruginosastrain is capable of biodegradation of surgical face mask made up of hardy PP like substance.…”

“…Similar ndings were reported in surface morphology of the LDPE lms treated with Pseudomonas spp. by SEM after 40, 80 and 120 days of incubation (Kyawet al, 2012).The another study also reported that the P.aeruginosa ISJ14 used to treat LDPE lm showed maximum deterioration after 60 days of treatment when observed under the FE-SEM (Gupta and Devi, 2020). A cross reference to the earlier research studies on LDPE biodegradation, many authors have reported the similar morphological changes on LDPE degradation by Aspergillusspp.…”

“…The metal strips and ear loops were removed from the masks. Then the masks were pretreated by the method previously described by Gupta and Devi (2020). For this process the masks were cut into (3×3 cm) pieces and soaked into the solution containing 7ml of Tween-80, 10ml of bleach and 983ml of sterile water for 30-60 minutes with continuous stirring.…”

“…The microbial exo-enzymes can breaks the complex polymers to simpler one which was then easily pass through the semi-permeable outer membranes and then it can be used as carbon and energy sources of the microbes (Gu, 2003). A number of bacterial and fungal genera were reported by several researchers for microplastic degradation process including Pseudomonas sp (Gupta and Devi, 2020;Vague et al, 2019;Muniret al, 2018;Roberts et al, 2020). The ability of microbes to mediate plastic degradation directly there are several gut bacterial species were isolated from worms and veri ed.…”