Characteristics and Utility of Human Hematopoietic Cell Lines

Minowada, Jun

doi:10.1007/978-3-642-74227-9_2

Cited by 1 publication

(6 citation statements)

References 54 publications

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“…The cell line Nalm-20 has rearrangements at the TCR g locus and deletions at the TCRˇlocus. The original diagnosis in the patient from which Nalm-20 was derived was biphenotypic ALL [19]. The phenotypic characterization of Nalm-20 is consistent with a biphenotypic acute leukemia because it has features characteristic of both the indicates that the cell line tested positive or negative, respectively, for V(D)J recombination activity using the pGG49 (SV40-based) episomal V(D)J substrate as described in Gauss and Lieber [36].…”

Section: V(d)j Recombinase Activity In Biphenotypic Neoplastic Cellsmentioning

confidence: 97%

“…V(D)J recombination activity was found in three cell lines in the myeloid lineages, K562, Nalm-20 and HL-60 [19]. The cell line Nalm-20 has rearrangements at the TCR g locus and deletions at the TCRˇlocus.…”

Section: V(d)j Recombinase Activity In Biphenotypic Neoplastic Cellsmentioning

confidence: 97%

“…The antigen receptor loci of K562 and HL-60 are germ line [19], illustrating that the V(D)J recombinase activity can be present, but the chromosomal antigen receptor loci may not be accessible for recombination.…”

Section: V(d)j Recombinase Activity In Biphenotypic Neoplastic Cellsmentioning

confidence: 98%

“…The phenotypic characterization of Nalm-20 is consistent with a biphenotypic acute leukemia because it has features characteristic of both the indicates that the cell line tested positive or negative, respectively, for V(D)J recombination activity using the pGG49 (SV40-based) episomal V(D)J substrate as described in Gauss and Lieber [36]. Cell lines are grouped according to proposed B cell differentiation stages [37], using markers reported in the FCCH catalog of human hematopoietic cell lines [19], with the modifications of Yoneda et al [22]. The markers of P30/Ohkubo and HPB-NULL encompass several differentiation stages, which are indicated by bars.…”

Section: V(d)j Recombinase Activity In Biphenotypic Neoplastic Cellsmentioning

confidence: 99%

“…Thirty-five human lymphoid and myeloid cell lines [17][18][19] were tested for V(D)J recombination activity using extrachromosomal V(D)J substrates. Nine lymphoid cell lines and three myeloid cell lines were positive for V(D)J recombination (Table 1).…”

Section: V(d)j Recombinase Activity In Human Neoplastic Hematopoieticmentioning

confidence: 99%

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V(D)J recombination activity in human hematopoietic cells: correlation with developmental stage and genome stability

et al. 1998

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V(D)J recombinase activity was measured in an array of human cell lines derived from hematopoietic malignancies representing various lineages and developmental stages. The level of recombinase activity was found to vary over a 2000-fold range between different cell lines. Several myeloid cell lines were positive for V(D)J recombinase activity, providing additional insight into the relationship between myeloid and lymphoid differentiation. Despite high levels of V(D)J recombination in two human acute lymphoblastic leukemia cell lines, the cytogenetic karyotype has remained essentially constant over several years of continuous cell culture. Silencing of recombination of chromosomal and minichromosomal targets has been strongly correlated with the replication of CpG methylated DNA in murine cells. Here, in human cells, we show that human minichromosomes bearing V(D)J recombination signals are protected well over 100-fold from recombination if they are CpG methylated, providing a rational basis for the karyotypic stability in cells with high levels of V(D)J recombination activity.

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Section: V(d)j Recombinase Activity In Biphenotypic Neoplastic Cellsmentioning

confidence: 97%

Section: V(d)j Recombinase Activity In Biphenotypic Neoplastic Cellsmentioning

confidence: 97%

Section: V(d)j Recombinase Activity In Biphenotypic Neoplastic Cellsmentioning

confidence: 98%

Section: V(d)j Recombinase Activity In Biphenotypic Neoplastic Cellsmentioning

confidence: 99%

Section: V(d)j Recombinase Activity In Human Neoplastic Hematopoieticmentioning

confidence: 99%

See 3 more Smart Citations

V(D)J recombination activity in human hematopoietic cells: correlation with developmental stage and genome stability

et al. 1998

View full text Add to dashboard Cite

show abstract

Characteristics and Utility of Human Hematopoietic Cell Lines

Cited by 1 publication

References 54 publications

V(D)J recombination activity in human hematopoietic cells: correlation with developmental stage and genome stability

V(D)J recombination activity in human hematopoietic cells: correlation with developmental stage and genome stability

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