Characteristics and thermodynamics of the interaction of 6-shogaol with human serum albumin as studied by isothermal titration calorimetry

Feroz, Shevin Rizal; Malek, Sri Nurestri Abd; Tayyab, Saad

doi:10.1590/s1984-82502016000300010

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…[ 25 ] In our studied system, the Δ H of this first equilibrium was high, Δ H = (–6.69 ± 0.05) × 10 4 cal mol –1 , indicating that another kind of binding was acting together with the electrostatic binding. This extra energy registered could correspond to hydrogen bonding between the head of the CTAB molecules and the rhEGF protein [ 26 ] or a reorganization of the rhEGF structure upon contact with the Blank@Quatsomes. As was previously evidenced by both CD and intrinsic Trp fluorescence, aromatic amino acids are probably implicated in rhEGF binding to the quatsome surface.…”

Section: Resultsmentioning

confidence: 99%

Recombinant Human Epidermal Growth Factor/Quatsome Nanoconjugates: A Robust Topical Delivery System for Complex Wound Healing

Ferrer-Tasies

Santana

Cabrera-Puig

et al. 2021

Advanced Therapeutics

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A multitude of microparticles and nanoparticles is developed to improve the delivery of different small drugs and large biomolecules, which are subject to several hindering biological barriers that limit their optimal biodistribution and therapeutic effects. Here, a soft, reliable, and scalable method based on compressed CO2 is reported for obtaining nanoconjugates of recombinant human epidermal growth factor and nanovesicles called quatsomes, where the latter consists of cholesterol and cetyltrimethylammonium bromide. These nanoconjugates exhibit appropriate values of the major critical quality attributes of colloidal nanomedicines, such as controlled and narrow nanoscopic particle size distribution (which play important roles in determining their stability), drug loading, drug release, drug protection, targeting ability, and bioactivity. Also, they exhibit a dual action by 1) inbuilt antimicrobial activity preventing infections and 2) promoting regeneration of granulation tissue and re‐epithelialization with complete closure of complex wounds. Therefore, such nanoconjugates are a potential nanomedicine for the topical treatment of complex wounds, particularly diabetic foot ulcers and venous leg ulcers.

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Section: Resultsmentioning

confidence: 99%