Characteristics and Sampling Efficiencies of OMNI 3000 Aerosol Samplers

Kesavan, Jana; Schepers, Deborah

doi:10.21236/ada457464

Cited by 9 publications

(7 citation statements)

References 1 publication

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Air was sampled with a custommodified Omni 3000 fluid impinger (InnovaPrep LLC, Drexel, MO) modified to minimize tubing and allow full removal of tubing for cleaning and sterilization. Omni particle capture efficiency ranges from 40% for 0.5-m particles to Ͼ90% for Ͼ3-m particles (16). The device was chosen because of its relatively high capture rate of bacterium-sized particles; high rate of air passage (250 to 300 liters/min in this study), to minimize sampling time; and relatively quiet function, to minimize conspicuity during this public sampling campaign.…”

Section: Methodsmentioning

confidence: 99%

Culture-Independent Analysis of Aerosol Microbiology in a Metropolitan Subway System

Robertson

Baumgartner

Harris

et al. 2013

Appl Environ Microbiol

115

108

View full text Add to dashboard Cite

The goal of this study was to determine the composition and diversity of microorganisms associated with bioaerosols in a heavily trafficked metropolitan subway environment. We collected bioaerosols by fluid impingement on several New York City subway platforms and associated sites in three sampling sessions over a 1.5-year period. The types and quantities of aerosolized microorganisms were determined by culture-independent phylogenetic analysis of small-subunit rRNA gene sequences by using both Sanger (universal) and pyrosequencing (bacterial) technologies. Overall, the subway bacterial composition was relatively simple; only 26 taxonomic families made up ϳ75% of the sequences determined. The microbiology was more or less similar throughout the system and with time and was most similar to outdoor air, consistent with highly efficient air mixing in the system. Identifiable bacterial sequences indicated that the subway aerosol assemblage was composed of a mixture of genera and species characteristic of soil, environmental water, and human skin commensal bacteria. Eukaryotic diversity was mainly fungal, dominated by organisms of types associated with wood rot. Human skin bacterial species (at 99% rRNA sequence identity) included the Staphylococcus spp. Staphylococcus epidermidis (the most abundant and prevalent commensal of the human integument), S. hominis, S. cohnii, S. caprae, and S. haemolyticus, all well-documented human commensal bacteria. We encountered no organisms of public health concern. This study is the most extensive culture-independent survey of subway microbiota so far and puts in place pre-event information required for any bioterrorism surveillance activities or monitoring of the microbiological impact of recent subway flooding events.

show abstract

Section: Methodsmentioning

confidence: 99%

Culture-Independent Analysis of Aerosol Microbiology in a Metropolitan Subway System

Robertson

Baumgartner

Harris

et al. 2013

Appl Environ Microbiol

115

108

View full text Add to dashboard Cite

show abstract

“…The rest of UV radiation, from low to high energy, is categorized as UV-A (320-400 nm), UV-B (280-320 nm), and UV-C (100-280 nm), of which UV-C is considered to be the most germicidal. The UV-C wavelength between 250-270 nm can be effectively absorbed by microbial genetic material, and is the UV wavelength most lethal to microorganisms (Keyser et al, 2008). The germicidal effect of UV-C light on bacteria and viruses is primarily due to the formation of pyrimidine (thymine and cytosine) dimers that inhibit the replication and function of genetic material (Giese and Darby, 2000;Prescott et al, 2005).…”

Section: Environmental Factors Affecting Biological Decaymentioning

confidence: 99%

“…Filtration method would not encounter the problems (evaporation of sampling liquid and overloading) of impingers and impactors; however, long sampling duration by filtration may not benefit in microorganism collection because of the biological decay of microorganisms owing to dehydration (Griffin et al, 2011;Wang et al, 2001). In order to collect detectable amounts of microorganisms and to get representative samples over a longer period, some high-volume and durable samplers that can be continuously operated over hours have been developed and applied, including OMNI-3000 and high-volume impinger (Griffin et al, 2011;Kesavan and Schepers, 2006).…”

Section: Sampling Strategymentioning

confidence: 99%

Airborne Microorganisms From Livestock Production Systems and Their Relation to Dust

Zhao

Aarnink

Jong

et al. 2014

Critical Reviews in Environmental Science and Technology

View full text Add to dashboard Cite

Large amounts of airborne microorganisms are emitted from livestock production. These emitted microorganisms may associate with dust, and are suspected to pose a risk of airborne infection to humans in vicinity and to animals on other farms. However, the extent to which airborne transmission may play a role in the epidemic, and how dust acts as a carrier of microorganisms in the transmission processes is unknown. The authors present the current knowledge of the entire process of airborne transmission of microorganisms-from suspension and transportation until deposition and infection-and their relation to dust. The sampling and the mitigation techniques of airborne microorganisms and dust in livestock production systems are introduced as well.Airborne Microorganisms and Dust in Livestock Houses 1073 airborne microorganisms and dust from livestock production systems, as well as the factors affecting their concentrations. In section 3, the physical and biological decay of airborne microorganisms and dust during transmission is described. In section 4, the deposition of airborne microorganisms and dust in respiratory tracts, and the infective dose of pathogenic microorganisms to animals are introduced. In section 5, the strategies and techniques for sampling microorganisms and dust in livestock production systems are proposed. In section 6, the mitigation techniques are described.

show abstract

“…To estimate the number of collectable biological particles, collection efficiencies (CE) were gathered from independent windtunnel testing conducted by the U.S. government using a range of polystyrene latex beads between 0.5 and 8 microns in diameter [36]. The aerosol collection rate employed by Kesavan et al (2006) was 277 liters per minute, which is sufficiently close to 300 liters per minute. We applied CE from 3 and 0.5 microns beads corresponding to 0.91 and 0.357 for the bacterial and viral calculations, respectively.…”

Section: Particle Collection Estimatesmentioning

confidence: 99%

An Analysis on the Detection of Biological Contaminants Aboard Aircraft

2011

View full text Add to dashboard Cite

The spread of infectious disease via commercial airliner travel is a significant and realistic threat. To shed some light on the feasibility of detecting airborne pathogens, a sensor integration study has been conducted and computational investigations of contaminant transport in an aircraft cabin have been performed. Our study took into consideration sensor sensitivity as well as the time-to-answer, size, weight and the power of best available commercial off-the-shelf (COTS) devices. We conducted computational fluid dynamics simulations to investigate three types of scenarios: (1) nominal breathing (up to 20 breaths per minute) and coughing (20 times per hour); (2) nominal breathing and sneezing (4 times per hour); and (3) nominal breathing only. Each scenario was implemented with one or seven infectious passengers expelling air and sneezes or coughs at the stated frequencies. Scenario 2 was implemented with two additional cases in which one infectious passenger expelled 20 and 50 sneezes per hour, respectively. All computations were based on 90 minutes of sampling using specifications from a COTS aerosol collector and biosensor. Only biosensors that could provide an answer in under 20 minutes without any manual preparation steps were included. The principal finding was that the steady-state bacteria concentrations in aircraft would be high enough to be detected in the case where seven infectious passengers are exhaling under scenarios 1 and 2 and where one infectious passenger is actively exhaling in scenario 2. Breathing alone failed to generate sufficient bacterial particles for detection, and none of the scenarios generated sufficient viral particles for detection to be feasible. These results suggest that more sensitive sensors than the COTS devices currently available and/or sampling of individual passengers would be needed for the detection of bacteria and viruses in aircraft.

show abstract

Characteristics and Sampling Efficiencies of OMNI 3000 Aerosol Samplers

Cited by 9 publications

References 1 publication

Culture-Independent Analysis of Aerosol Microbiology in a Metropolitan Subway System

Culture-Independent Analysis of Aerosol Microbiology in a Metropolitan Subway System

Airborne Microorganisms From Livestock Production Systems and Their Relation to Dust

An Analysis on the Detection of Biological Contaminants Aboard Aircraft

Contact Info

Product

Resources

About