Characteristics and metabolic patterns of soil methanogenic archaea communities in the high‐latitude natural forested wetlands of China

Wu, Di; Zhao, Caihong; Bai, Hui; Feng, Fude; Sui, Xin; Guo-rong, Sun

doi:10.1002/ece3.7842

Cited by 6 publications

(8 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the methanogenic orders identified here as having potential to catalyze methylotrophy, only the Methanomassiliicoccales appear to be functioning as obligate methylotrophs, likely using these substrates dependent on hydrogen as an electron donor (7). Studies have demonstrated that these methanogens are cosmopolitan across water inundated soils and sediments (14,(58)(59)(60). In contrast to prior metagenomic studies (14,58) we did not infer that methanol was the preferential substrate for this lineage.…”

Section: Methylotrophic Methanogenesis Is Encoded By Much Of the Nati...contrasting

confidence: 72%

“…Beyond the Methanomassiliicoccales, we expanded the observation of methylotrophy within this site to include members of the Methanosarcinales and Methanobacteriales, identifying these lineages as primarily methanol-utilizing facultative methylotrophs. Both orders have been identified as members of methanogenic communities across global wetlands (60,(62)(63)(64). However, the Methanobacteriales are typically classified solely as hydrogenotrophs (60,63), whereas the Methanosarcinales are inconsistently labeled as acetoclasts (62), acetoclastic and hydrogenotrophic (60), or metabolically versatile (e.g., capable of all modes of methanogenesis) (14,63).…”

Section: Methylotrophic Methanogenesis Is Encoded By Much Of the Nati...mentioning

confidence: 99%

“…Both orders have been identified as members of methanogenic communities across global wetlands (60,(62)(63)(64). However, the Methanobacteriales are typically classified solely as hydrogenotrophs (60,63), whereas the Methanosarcinales are inconsistently labeled as acetoclasts (62), acetoclastic and hydrogenotrophic (60), or metabolically versatile (e.g., capable of all modes of methanogenesis) (14,63). Prior, two Methanobacterium isolates (24, 46) -one of which is from a permafrost system (46) -have been shown to perform hydrogen-dependent methylotrophy using methanol and/or methylamines.…”

Section: Methylotrophic Methanogenesis Is Encoded By Much Of the Nati...mentioning

confidence: 99%

See 2 more Smart Citations

Methylotrophy in the Mire: direct and indirect routes for methane production in thawing permafrost

Ellenbogen¹,

Borton²,

McGivern³

et al. 2023

Preprint

View full text Add to dashboard Cite

While wetlands are major sources of biogenic methane (CH4), our understanding of resident microbial metabolisms is incomplete, which compromises prediction of CH4 emissions under ongoing climate change. Here, we employed genome-resolved multi-omics to expand our understanding of methanogenesis in the thawing permafrost peatland of Stordalen Mire, in arctic Sweden. In quadrupling the genomic representation of the site's methanogens and examining their encoded metabolisms, we revealed that nearly 20% (72) of the metagenome-assembled genomes (MAGs) encoded potential for methylotrophic methanogenesis. Further, 27% of the transcriptionally active methanogens expressed methylotrophic genes; for Methanosarcinales and Methanobacteriales MAGs, these data indicated use of methylated oxygen compounds (e.g., methanol), while for Methanomassiliicoccales, they primarily implicated methyl sulfides and methylamines. In addition to methanogenic methylotrophy, >1700 bacterial MAGs across 19 phyla encoded anaerobic methylotrophic potential, with expression across 12 phyla. Metabolomic analyses revealed the presence of diverse methylated compounds in the Mire, including some known methylotrophic substrates. Active methylotrophy was observed across all stages of a permafrost thaw gradient in Stordalen, with the most frozen non-methanogenic palsa found to host bacterial methylotrophy, and the partially thawed bog and fully thawed fen seen to house both methanogenic and bacterial methylotrophic activity. Methanogenesis across increasing permafrost thaw is thus revised from sole dominance of hydrogenotrophic production, and the appearance of acetoclastic at full thaw, to consider co-occurrence of methylotrophy throughout. Collectively, these findings indicate that methanogenic and bacterial methylotrophy may be an important and previously underappreciated component of carbon cycling and emissions in these rapidly changing wetland habitats.

show abstract

Section: Methylotrophic Methanogenesis Is Encoded By Much Of the Nati...contrasting

confidence: 72%

Section: Methylotrophic Methanogenesis Is Encoded By Much Of the Nati...mentioning

confidence: 99%

Section: Methylotrophic Methanogenesis Is Encoded By Much Of the Nati...mentioning

confidence: 99%

See 1 more Smart Citation

Methylotrophy in the Mire: direct and indirect routes for methane production in thawing permafrost

Ellenbogen¹,

Borton²,

McGivern³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…This was the only family to be overrepresented on a genotype across both soil environments. The Micropepsaceae has been found to be an indicator taxon associated with specific plant species or site conditions in other studies (D. Wu et al 2023; Gschwend et al 2022). For studies that compared different environmental manipulations, the family was associated with early phases of restoration (grasslands; (Barber et al 2023)), early responses to agricultural planting (tobacco; (Cao et al 2022)), and response to cold treatments of plants (lettuce; (Persyn et al 2022)), all conditions in which a system had been perturbed recently.…”

Section: Discussionmentioning

confidence: 71%

Plant G x Microbial E: Plant genotype interaction with soil bacterial community shapes rhizosphere composition during invasion

Berlow,

Mesa,

Creek

et al. 2024

Preprint

View full text Add to dashboard Cite

It is increasingly recognized that different genetic variants can uniquely shape their microbiomes. Invasive species often evolve in their introduced ranges, but little is known about the potential for microbial associations to evolve during invasion as a result. We investigated invader genotype (G) and microbial environment (E) interactions in C. solstitialis (yellow starthistle), a Eurasian plant that is known to have evolved novel genotypes, and to have altered microbial interactions, in its severe invasion of California, USA. We conducted an experiment in which native and invading genotypes were inoculated with native and invaded range soil microbial communities. We used amplicon sequencing to characterize rhizosphere bacteria in both the experiment and the field soils from which they were derived. We found that bacterial diversity is higher in invaded soils, but that invading genotypes accumulated a lower diversity of bacteria and unique microbial composition in experimental inoculations, relative to native genotypes. Associations with potentially beneficial Streptomycetaceae were particularly interesting, as these were more abundant in the invaded range and accumulated on invading genotypes. Thus variation in microbial associations of invaders was driven by the interaction of G and E, and microbial communities appear to change in composition along with host evolution during invasion.

show abstract

“…A previous study proved that 35–44% of the CH 4 output was observed from the CO 2 pathway in peatland (Kotsyurbenko et al, 2004 ). Hydrogenotrophic methanogenesis was also observed as the most dominant pathway in forest wetlands (Wu et al, 2021 ). In our study, the percentage of CO 2 pathway genes accounted for 20% in wetland and 15% in cropland ( Figures 4A , B ).…”

Section: Discussionmentioning

confidence: 99%

Metagenomic evidence of suppressed methanogenic pathways along soil profile after wetland conversion to cropland

Wang

Zhu²,

Zuo³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Wetland conversion to cropland substantially suppresses methane (CH4) emissions due to the strong suppression of methanogenesis, which consists of various pathways. In this study, we evaluated the cultivation impacts on four predominant CH4 production pathways, including acetate, carbon dioxide (CO2), methylamines, and methanol, in a wetland and cultivated cropland in northeastern China. The results showed significant suppression of CH4 production potential and the abundance of genes for all four methanogenic pathways in cropland. The consistency between CH4 production and methanogenesis genes indicates the robustness of genomic genes in analyzing methanogenesis. The suppression effects varied across seasons and along soil profiles, most evident in spring and 0 to 30 cm layers. The acetate pathway accounted for 55% in wetland vs. 70% in the cropland of all functional genes for CH4 production; while the other three pathways were stronger in response to cultivation, which presented as stronger suppressions in both abundance of functional genes (declines are 52% of CO2 pathway, 68% of methanol pathway, and 62% of methylamines pathway, vs. 19% of acetate pathway) and their percentages in four pathways (from 20 to 15% for CO2, 15 to 9% for methylamines, and 10 to 6% for methanol pathway vs. 55 to 70% for acetate pathway). The structural equation models showed that substrate availability was most correlated with CH4 production potential in the wetland, while the positive correlations of acetate, CO2, and methylamine pathways with CH4 production potential were significant in the cropland. The quantitative responses of four CH4 production pathways to land conversion reported in this study provide benchmark information for validating the CH4 model in simulating CH4 cycling under land use and land cover change.

show abstract

Characteristics and metabolic patterns of soil methanogenic archaea communities in the high‐latitude natural forested wetlands of China

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 6 publications

References 68 publications

Methylotrophy in the Mire: direct and indirect routes for methane production in thawing permafrost

Methylotrophy in the Mire: direct and indirect routes for methane production in thawing permafrost

Plant G x Microbial E: Plant genotype interaction with soil bacterial community shapes rhizosphere composition during invasion

Metagenomic evidence of suppressed methanogenic pathways along soil profile after wetland conversion to cropland

Contact Info

Product

Resources

About