Characteristics and metabolic pathway of acetamiprid biodegradation by Fusarium sp. strain CS-3 isolated from soil

Shi, Zhoukun; Dong, Weiliang; Xin, Fengxue; Liu, Jiawei; Zhou, Xue; Xu, Fanli; Lv, Ziyao; Ma, Jiangfeng; Zhang, Wenming; Fang, Yan; Jiang, Min

doi:10.1007/s10532-018-9855-8

Cited by 28 publications

(12 citation statements)

References 38 publications

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“…Microbiological degradation plays key roles in the dissipation of acetamiprid from the environment, as well as in the detoxification of acetamiprid. Many prokaryotic and eukaryotic microorganisms have been reported to be capable of degrading acetamiprid (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28). Strain D-2, previously isolated in our laboratory, could degrade over 99% of 0.22 mM acetamiprid in 3 days, showing that it possesses a very high degradation capability.…”

Section: Discussionmentioning

confidence: 85%

“…The environmental fate of acetamiprid has attracted considerable attention, and microbial degradation is the key means for the dissipation of acetamiprid from the environment (15,16). In recent years, several microbial strains capable of degrading acetamiprid have been isolated, and the catabolic pathways of acetamiprid have been extensively studied (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28). Four catabolic pathways of acetamiprid are illustrated in Fig.…”

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confidence: 99%

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Pigmentiphaga sp. Strain D-2 Uses a Novel Amidase To Initiate the Catabolism of the Neonicotinoid Insecticide Acetamiprid

Yang

Wang

et al. 2020

Appl Environ Microbiol

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Acetamiprid, a chloronicotinyl neonicotinoid insecticide, is among the most commonly used insecticides worldwide, and its environmental fate has caused considerable concern. The compound 1-(6-chloropyridin-3-yl)-N-methylmethanamine (IM 1-4) has been reported to be the main intermediate during acetamiprid catabolism in microorganisms, honeybees, and spinach. However, the molecular mechanism underlying the hydrolysis of acetamiprid to IM 1-4 has not yet been elucidated. In this study, a novel amidase (AceAB) that initially hydrolyzes the C-N bond of acetamiprid to generate IM 1-4 was purified and characterized from the acetamiprid-degrading strain Pigmentiphaga sp. strain D-2. Based on peptide profiling of the purified AceAB and the draft genome sequence of strain D-2, aceA (372 bp) and aceB (2,295 bp), encoding the α and β subunits of AceAB, respectively, were cloned and found to be necessary for acetamiprid hydrolysis in strain D-2. The characteristics of AceAB were also systematically investigated. Though AceA and AceB showed 35% to 56% identity to the α and β subunits of the N,N-dimethylformamidase from Paracoccus aminophilus, AceAB was specific for the hydrolysis of acetamiprid and showed no activities to N,N-dimethylformamide or its structural analogs. IMPORTANCE Acetamiprid, among the top neonicotinoid insecticides used worldwide, is one of the most important commercial insecticides. Due to its extensive use, the environmental fate of acetamiprid, especially its microbial degradation, has caused considerable concern. Although the catabolic pathways of acetamiprid in microorganisms have been extensively studied, the molecular mechanisms underlying acetamiprid biodegradation (except for a nitrile hydratase) remain largely unknown, and the enzyme responsible for the biotransformation of acetamiprid into its main intermediate, IM 1-4, have not yet been elucidated. The amidase AceAB and its encoding genes, aceA and aceB, characterized in this study, were found to be necessary and specific for the initial hydrolysis of the C-N bond of acetamiprid to generate IM 1-4 in Pigmentiphaga sp. strain D-2. The finding of the novel amidase AceAB will greatly enhance our understanding of the microbial catabolism of the widely used insecticide acetamiprid at the molecular level.

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Section: Discussionmentioning

confidence: 85%

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confidence: 99%

Pigmentiphaga sp. Strain D-2 Uses a Novel Amidase To Initiate the Catabolism of the Neonicotinoid Insecticide Acetamiprid

Yang

Wang

et al. 2020

Appl Environ Microbiol

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“…Anhalt (9) reported 67% of the initial pesticide dose was degraded within 48 hours at optimum pH 8 and temperature 30°C. Shi (22) reported Fusarium sp. strain CS-3 could grow at optimum temperature of 30°C and pH 7.0.…”

Section: Discussionmentioning

confidence: 99%

Imidacloprid Degradation Potential Of Bacillus Strain Isolated From Agriculture Soil

Naseer¹,

Nazuk²,

Iqbal³

2020

JMMG

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Continuous dispersion of pesticide in the environment and its stability in soil results in environmental pollution that cause harmful effect to living organisms and human by entering in food chain and this hazardous problem need to eradicate by remediation. The present research was attempted to isolate and characterize imidacloprid (IMIDA) degrading Bacillus strains from the different areas of Multan region. The imidacloprid resistant strains were isolated by checking their imidacloprid degradation ability in minimal salt medium (MSM). Levels of imidacloprid in MSM medium were analyzed by thin layer chromatography (TLC) and Bacillus mediated degradation of imidacloprid was assessed by using fourier transform infrared spectroscopy (FTIR). Out of 38 imidacloprid resistant strains, from 6 different soil samples, A2 showed highest imidacloprid degrading ability. The Bacillus strain A2 showed maximum growth at 25°C and pH 7 (at 600 nm). Degradation of IMIDA was determined by Rf values for IMIDA containing control (0.72) and culture extracts (0.8, 0.54 and 0.3) in TLC analysis. FTIR spectrum of strain A2 showed the appearance of new peaks at 2253 cm-1 from 2500 to 2000 cm-1 peak area as compared to control. The shifts of the peak pattern as well as occurrence of few new peaks attribute to biodegradation of imidacloprid after bacterial treatment. This study has shown that soil microbial activity significantly affected the technical profile of imidacloprid insecticides after soil application.

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“…Notably, the nutrient conditions in the natural environment are relatively inadequate, and cannot guarantee the efficient growth of bacteria with the desired degradation ability. Moreover, the more complex degradation conditions (e.g., swings of temperature, pH, and oxygen) may be unfavorable to the screened mixed bacteria cultures (Shi et al, 2018). In addition, the nutrient competition and even parasitism by other microorganisms may lead to instability or even the disappearance of the biodegrading bacteria (Ramanan et al, 2015).…”

Section: Analysis Of the Bacterial Communitymentioning

confidence: 99%

“…BCH2 (Phugare and Jadhav, 2015), and Fusarium sp. CS-3 (Shi et al, 2018). Among these degraders, strains of Stenotrophomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Acetamiprid Biodegradation by the Microbial Consortium ACE-3 Enriched From Contaminated Soil

Xue

Zhou

et al. 2020

Front. Microbiol.

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Microbial consortia are ubiquitous in nature and exhibit several attractive features such as sophisticated metabolic capabilities and strong environmental robustness. This study aimed to decipher the metabolic and ecological characteristics of synergistic interactions in acetamiprid-degrading consortia, suggesting an optimal scheme for bioremediation of organic pollutants. The microbial consortium ACE-3 with excellent acetamiprid-degrading ability was enriched from the soil of an acetamiprid-contaminated site and characterized using high-throughput sequencing (HTS). Consortium ACE-3 was able to completely degrade 50 mg•L −1 acetamiprid in 144 h, and was metabolically active at a wide range of pH values (6.0-8.0) and temperatures (20-42 • C). Furthermore, plausible metabolic routes of acetamiprid biodegradation by the consortium were proposed based on the identification of intermediate metabolites (Compounds I, II, III and IV). The findings indicated that the consortium ACE-3 has promising potential for the removal and detoxification of pesticides because it produces downstream metabolites (Compounds I and II) that are less toxic to mammals and insects than acetamiprid. Finally, Illumina HTS revealed that β Proteobacteria were the dominant group, accounting for 85.61% of all sequences at the class level. Among the more than 50 genera identified in consortium ACE-3, Sphingobium, Acinetobacter, Afipia, Stenotrophomonas, and Microbacterium were dominant, respectively accounting for 3.07, 10.01, 24.45, and 49.12% of the total population.

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Characteristics and metabolic pathway of acetamiprid biodegradation by Fusarium sp. strain CS-3 isolated from soil

Cited by 28 publications

References 38 publications

Pigmentiphaga sp. Strain D-2 Uses a Novel Amidase To Initiate the Catabolism of the Neonicotinoid Insecticide Acetamiprid

Pigmentiphaga sp. Strain D-2 Uses a Novel Amidase To Initiate the Catabolism of the Neonicotinoid Insecticide Acetamiprid

Imidacloprid Degradation Potential Of Bacillus Strain Isolated From Agriculture Soil

Characterization of Acetamiprid Biodegradation by the Microbial Consortium ACE-3 Enriched From Contaminated Soil

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