Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans

Zhou, Xin; Wang, Xing; Cao, Rou; Tao, Yuheng; Xu, Yong; Yu, Shiyuan

doi:10.15376/biores.10.3.4277-4286

Cited by 7 publications

(2 citation statements)

References 21 publications

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“… 1,14,15 In fact, the introduction of these aldonic acids into commerce is still limited due to the low efficiency of currently reported biocatalysts. 16 Therefore, further screening and exploration of strains with novel biocatalytic activities plays an important role in the development of new processes for aldonic acid production that are both sustainable and efficient.…”

Section: Introductionmentioning

confidence: 99%

Efficient production of sugar-derived aldonic acids byPseudomonas fragiTCCC11892

et al. 2018

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Aldonic acids are receiving increased interest due to their applications in nanotechnology, food, pharmaceutical and chemical industries. Microbes with aldose-oxidizing activity, rather than purified enzymes, are used for commercial production with limited success. Thus it is still very important to develop new processes using strains with more efficient and novel biocatalytic activities for the production of adonic acids. In the present study, Pseudomonas fragi TCCC11892 was found to be an efficient producer of aldonic acids, with the production of galactonic and L-rhamnonic acid by P. fragi reported for the first time. The semi-continuous production of maltobionic acid and lactobionic acid was developed for P. fragi TCCC11892, achieving a yield of over 90 g L À1 for the first 7 cycles. The excellent performance of P. fragi in the production of lactobionic acid (119 g L À1 ) was also observed when using waste cheese whey as an inexpensive fermentation medium. Scaling up of the above process for production of aldonic acids with P. fragi TCCC11892 cells should facilitate their commercial applications.

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Section: Introductionmentioning

confidence: 99%

Efficient production of sugar-derived aldonic acids byPseudomonas fragiTCCC11892

et al. 2018

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“…However, in general, the fermentation process profile of G. oxydans was very similar in both mediums. Also, the glucose conversion followed the same pattern: the fermentation was divided into two stages, including the conversion of glucose to GA and further metabolism of xylose and GA, where xylose was bio-oxidized into XA and GA was partly converted to 2-KGA [39]. This phenomenon suggested that it is feasible to manually stop the reaction when the glucose is consumed by G. oxydans, thus retaining GA and xylose in the fermentation broth.…”

Section: The Kinetics Of G Oxydans For the Mixture Medium Of Glucose ...mentioning

confidence: 97%

Sequential Bioprocess with Gluconobacter oxydans and Candida tropicalis for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate

et al. 2023

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To meet the growing global demand for gluconic acid as a cement and concrete retarder, inexpensive and abundant lignocellulosic materials are regarded as the most suitable alternatives to starchy materials. However, their enzymatic hydrolysate contains not only glucose but also xylose, which negatively affects the performance of gluconic acid as a retarder. Notably, glucose is preferentially bio-oxidized into gluconic acid by Gluconobacter oxydans, but gluconic acid cannot be metabolized by Candida tropicalis. Given this, an artificially designed biological cascade process, respectively employing Gluconobacter oxydans and Candida tropicalis, was established to successfully carry out glucose conversion into gluconic acid, and xylose into a single-cell protein, using the enzymatic hydrolysate of corncobs as a feedstock. This sequential fermentation process produced 95.8 g/L gluconic acid and 9.0 g/L single-cell protein from one liter of the enzymatic hydrolysate that initially contained 98.1 g/L of glucose and 25.4 g/L of xylose. The mass-balance calculation showed that approximately 280 grams of gluconic acid and 27 grams of the single-cell protein could be harvested from 1000 grams of the corncob feedstock. The results suggest that the above-mentioned two-step bioconversion method is efficient in utilizing glucose and xylose from lignocellulosic hydrolysates.

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Integrated production of gluconic acid and xylonic acid using dilute acid pretreated corn stover by two-stage fermentation

Zhou

Liu

et al. 2018

Biochemical Engineering Journal

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Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans

Cited by 7 publications

References 21 publications

Efficient production of sugar-derived aldonic acids byPseudomonas fragiTCCC11892

Efficient production of sugar-derived aldonic acids byPseudomonas fragiTCCC11892

Sequential Bioprocess with Gluconobacter oxydans and Candida tropicalis for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate

Integrated production of gluconic acid and xylonic acid using dilute acid pretreated corn stover by two-stage fermentation

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