Characteristics and Functions of MYB (v-Myb avivan myoblastsis virus oncogene homolog)-Related Genes in Arabidopsis thaliana

Wu, Guofan; Cao, Aohua; Wen, Yuhan; Bao, Wencheng; She, Fawen; Wu, Wangze; Zheng, Sheng; Yang, Ning

doi:10.3390/genes14112026

Cited by 2 publications

(3 citation statements)

References 113 publications

(127 reference statements)

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“…The MYB-related transcription factor family, as a subfamily of MYB transcription factors, is widely present in plants. However, distinct from another subfamily, the R2R3-MYB, the MYB-related transcription factors have not been as extensively characterized [ 32 ]. Despite this, recent studies have begun to unravel the functions of the MYB-related transcription factors.…”

Section: Discussionmentioning

confidence: 99%

“…Despite numerous studies on the R2R3-MYB gene family, there are few reports on the functions of MYB-related genes. The MYB-related transcription factor, which contains the MYB/SANT domain, is a subclass of the MYB family and is widely distributed in plants [ 28 – 32 ]. Previous studies have shown that the MYB transcription factor family primarily regulates stress and abiotic stress responses by participating in redox reactions and hormone synthesis [ 33 – 35 ].…”

Section: Introductionmentioning

confidence: 99%

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A MYB-related transcription factor ZmMYBR29 is involved in grain filling

Wu,

Wang,

Yan

et al. 2024

BMC Plant Biol

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Background The endosperm serves as the primary source of nutrients for maize (Zea mays L.) kernel embryo development and germination. Positioned at the base of the endosperm, the transfer cells (TCs) of the basal endosperm transfer layer (BETL) generate cell wall ingrowths, which enhance the connectivity between the maternal plant and the developing kernels. These TCs play a crucial role in nutrient transport and defense against pathogens. The molecular mechanism underlying BETL development in maize remains unraveled. Results This study demonstrated that the MYB-related transcription factor ZmMYBR29, exhibited specific expression in the basal cellularized endosperm, as evidenced by in situ hybridization analysis. Utilizing the CRISPR/Cas9 system, we successfully generated a loss-of-function homozygous zmmybr29 mutant, which presented with smaller kernel size. Observation of histological sections revealed abnormal development and disrupted morphology of the cell wall ingrowths in the BETL. The average grain filling rate decreased significantly by 26.7% in zmmybr29 mutant in comparison to the wild type, which impacted the dry matter accumulation within the kernels and ultimately led to a decrease in grain weight. Analysis of RNA-seq data revealed downregulated expression of genes associated with starch synthesis and carbohydrate metabolism in the mutant. Furthermore, transcriptomic profiling identified 23 genes that expressed specifically in BETL, and the majority of these genes exhibited altered expression patterns in zmmybr29 mutant. Conclusions In summary, ZmMYBR29 encodes a MYB-related transcription factor that is expressed specifically in BETL, resulting in the downregulation of genes associated with kernel development. Furthermore, ZmMYBR29 influences kernels weight by affecting the grain filling rate, providing a new perspective for the complementation of the molecular regulatory network in maize endosperm development.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A MYB-related transcription factor ZmMYBR29 is involved in grain filling

Wu,

Wang,

Yan

et al. 2024

BMC Plant Biol

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“…Cis-regulatory elements are essential in plant regulatory networks and offer insights into the functions of related genes [51]. They have been identified as candidates involved in salt tolerance [52], the response to low-temperature stress [49], and drought induction [53].…”

Section: Similarity In the Structure Of F-box Genes At The S Locus In...mentioning

confidence: 99%

The Identification and Analysis of the Self-Incompatibility Pollen Determinant Factor SLF in Lycium barbarum

Wu,

Nan,

Zhang

et al. 2024

Plants

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Self-incompatibility is a widespread genetic mechanism found in flowering plants. It plays a crucial role in preventing inbreeding and promoting outcrossing. The genes that control self-incompatibility in plants are typically determined by the S-locus female determinant factor and the S-locus male determinant factor. In the Solanaceae family, the male determinant factor is often the SLF gene. In this research, we cloned and analyzed 13 S2-LbSLF genes from the L. barbarum genome, which are located on chromosome 2 and close to the physical location of the S-locus female determinant factor S-RNase, covering a region of approximately 90.4 Mb. The amino acid sequence identity of the 13 S2-LbSLFs is 58.46%, and they all possess relatively conserved motifs and typical F-box domains, without introns. A co-linearity analysis revealed that there are no tandemly repeated genes in the S2-LbSLF genes, and that there are two pairs of co-linear genes between S2-LbSLF and the tomato, which also belongs to the Solanaceae family. A phylogenetic analysis indicates that the S2-LbSLF members can be divided into six groups, and it was found that the 13 S2-LbSLFs are clustered with the SLF genes of tobacco and Petunia inflata to varying degrees, potentially serving as pollen determinant factors regulating self-incompatibility in L. barbarum. The results for the gene expression patterns suggest that S2-LbSLF is only expressed in pollen tissue. The results of the yeast two-hybrid assay showed that the C-terminal region of S2-LbSLFs lacking the F-box domain can interact with S-RNase. This study provides theoretical data for further investigation into the functions of S2-LbSLF members, particularly for the identification of pollen determinant factors regulating self-incompatibility in L. barbarum.

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Characteristics and Functions of MYB (v-Myb avivan myoblastsis virus oncogene homolog)-Related Genes in Arabidopsis thaliana

Cited by 2 publications

References 113 publications

A MYB-related transcription factor ZmMYBR29 is involved in grain filling

A MYB-related transcription factor ZmMYBR29 is involved in grain filling

The Identification and Analysis of the Self-Incompatibility Pollen Determinant Factor SLF in Lycium barbarum

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