Characteristics and differences of gut microbiota in patients with different Traditional Chinese Medicine Syndromes of Colorectal Cancer and normal population

Abstract

Colorectal cancer (CRC) is considered to be closely associated with alteration of intestinal microorganisms. The purpose of present study was to investigate the distribution of gut microbiota in the distinction of microbiota dysbiosis between two disease syndromes called Zheng-Qi-Kui-Xu(ZQKX) and Xie-Du-Yong-Sheng (XDYS). First, From February 2019 to June 2019, CRC patients presenting to the oncology department of Zhejiang Province Hospital of TCM who met the established inclusion and exclusion criteria were enrolled in this prospective study. After fresh stool specimens of healthy volunteers and CRC patients with ZQKX or XDYS syndorme were collected, 16S rRNA gene amplification and sequencing could be used to identify the diversity and abundance of gut microbiota among groups. The results demonstrated that the composition of the microbiota in general control group was superior to those in experimental groups. At the phylum level, a significantly increased abundance of Bacteroides was observed in healthy volunteers. At the class level, Erysipelothrix decreased while Lactobacillaceae showed increased abundance in the ZQKX group compared to healthy controls. At the family level, Prevotella Shan and Collins decreased while Streptococcus significantly increased in patients with XDYS syndrome compared to healthy subjects. Five differential taxa were identified between ZQKX and XDYS syndromes. We suggest that the gut microbiota contributes to the distinction between the two TCM syndromes of CRC, which can be used as a biological basis of TCM syndrome differentiation treatment in CRC.

The interactions between traditional Chinese medicine and gut microbiota: Global research status and trends

“…GM in patients with spleen deficiency syndrome ( Peng et al., 2020 ), spleen-yang-deficiency syndrome ( Lin et al., 2018 ), and kidney-yang deficiency syndrome ( Chen et al., 2019 ) had changed, while TCM can help to restore the richness and diversity of GM in specific TCM syndromes ( Zheng et al., 2020 ), which provided insights into the correlation between TCM syndromes and GM imbalance and a new method for further studying the pathogenesis of GM regulating specific TCM syndrome. Two studies have shown that GM can help to distinguish the two TCM syndromes of UC ( Zhang et al., 2019 ) and colorectal cancer ( Wang et al., 2020 ), which can be used as the biological basis for TCM syndrome differentiation and treatment of related diseases. (2) Acupuncture and Moxibustion research: Moxibustion may promote the repair of gastric mucosal injury by increasing the number and types of intestinal beneficial bacteria ( He et al., 2019 ).…”

Proton pump inhibitors induce changes in the gut microbiome composition of systemic lupus erythematosus patients

“…To prepare gut microbiome library for sequencing, 16S rRNA were amplified at V3 to V4 hypervariable region by polymerase chain reaction (PCR). According to Illumina high-throughput sequencing requirements, two-way sequencing was performed, and primers were designed using (357f-806R) as the forward primer (5'-ACTCCTACGGRAGGCAGCAG-3’) and reverse primer (5'-GGACTACHVGGGTWTCTAAT-3') [ 31 ]. The two-step PCR amplification method was used to build a library.…”

Association of Gut Microbiota and Biochemical Features in a Chinese Population With Renal Uric Acid Stone

“…Fusobacteria is a kind of anaerobic Gram-negative bacteria, which is widely colonized in human intestinal and oral mucosa and is related to the invasion of tumor cells ( Kelly et al, 2018 ; Wang et al, 2020 ). There was a strong correlation between the abundance of Fusobacteria and the expression of pro-inflammatory markers such as COX-2, suggesting that Fusobacteria could create a pro-inflammatory micro-environment conducive to colorectal cancer by recruiting tumor-infiltrating immune cells ( Kostic et al, 2013 ; Sears, 2018 ; King et al, 2020 ).…”