Characteristics and Clinical Application of Extracellular Vesicle-Derived DNA

Hur, Jae Young; Lee, Kye Young

doi:10.3390/cancers13153827

Cited by 28 publications

(24 citation statements)

References 161 publications

(244 reference statements)

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“…The most promising application of EVs genomics is its biomarker use, as EVs DNA reflects the mutational status of parental cells, making the diagnosis and prognosis of different pathological conditions, such as tumors, easier and more accessible. This is supported also by the enhanced availability of EVs DNA in body fluids as protected from degradation by the EVs lipid bilayer [ 102 ]. Many examples are given by the extensive research on liquid biopsies of various cancers [ 103 ]; for instance, Takur et al reported the detection of specific colon carcinoma-associated mutations in plasma-derived EV-DNA; Lee et al tested the sensitivity in detecting epidermal growth factor receptor (EGFR) mutations in EVs-DNA from BALF of patients with advanced non-small cell lung cancer (NSCLC) by targeted next-generation sequencing (NGS) of both tissue DNA and vesicular DNA [ 104 ].…”

Section: Extracellular Vesicle As Resourceful Biological Systemsmentioning

confidence: 99%

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

et al. 2022

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In the era of multi-omic sciences, dogma on singular cause-effect in physio-pathological processes is overcome and system biology approaches have been providing new perspectives to see through. In this context, extracellular vesicles (EVs) are offering a new level of complexity, given their role in cellular communication and their activity as mediators of specific signals to target cells or tissues. Indeed, their heterogeneity in terms of content, function, origin and potentiality contribute to the cross-interaction of almost every molecular process occurring in a complex system. Such features make EVs proper biological systems being, therefore, optimal targets of omic sciences. Currently, most studies focus on dissecting EVs content in order to either characterize it or to explore its role in various pathogenic processes at transcriptomic, proteomic, metabolomic, lipidomic and genomic levels. Despite valuable results being provided by individual omic studies, the categorization of EVs biological data might represent a limit to be overcome. For this reason, a multi-omic integrative approach might contribute to explore EVs function, their tissue-specific origin and their potentiality. This review summarizes the state-of-the-art of EVs omic studies, addressing recent research on the integration of EVs multi-level biological data and challenging developments in EVs origin.

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Section: Extracellular Vesicle As Resourceful Biological Systemsmentioning

confidence: 99%

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

et al. 2022

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“…Exosomes can enter the recipient cell through receptor-ligand interactions ( Isaac et al, 2021 ; Staubach et al, 2021 ). Besides, exosomes can also be endocytosed by the recipient cells through phagocytosis ( Chen et al, 2021c ; Hur and Lee, 2021 ). Exosomes have an important role in tumor development and can serve as potential diagnostic markers and therapeutic targets for many tumors.…”

Section: Detection Of Exosomesmentioning

confidence: 99%

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Yang

Jia

2022

Front. Bioeng. Biotechnol.

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Exosomes are small nanoscale vesicles with a double-layered lipid membrane structure secreted by cells, and almost all types of cells can secrete exosomes. Exosomes carry a variety of biologically active contents such as nucleic acids and proteins, and play an important role not only in intercellular information exchange and signal transduction, but also in various pathophysiological processes in the human body. Surface-enhanced Raman Spectroscopy (SERS) uses light to interact with nanostructured materials such as gold and silver to produce a strong surface plasmon resonance effect, which can significantly enhance the Raman signal of molecules adsorbed on the surface of nanostructures to obtain a rich fingerprint of the sample itself or Raman probe molecules with ultra-sensitivity. The unique advantages of SERS, such as non-invasive and high sensitivity, good selectivity, fast analysis speed, and low water interference, make it a promising technology for life science and clinical testing applications. In this paper, we briefly introduce exosomes and the current main detection methods. We also describe the basic principles of SERS and the progress of the application of unlabeled and labeled SERS in exosome detection. This paper also summarizes the value of SERS-based exosome assays for early tumor diagnosis.

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“…Other possible EV cargos such as DNA or lipids have not been evaluated yet in MM. While circulating cell-free DNA could serve as a possible biomarker, the presence of double stranded DNA inside small EVs remains controversial, which can be attributed to discrepancies in the preparation method and size of the isolated EVs 107 . Lipids are important players in a variety of processes including membrane formation and intracellular signaling.…”

Section: Exosome Biomarkersmentioning

confidence: 99%

Exosomes in multiple myeloma: from bench to bedside

Vanderkerken

2022

Blood

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Multiple myeloma (MM) remains till today an incurable plasma cell malignancy which develops in the bone marrow (BM). This BM is partially responsible for protecting the MM cells against current standard-of-care therapies and for accommodating MM-related symptoms such as bone resorption and immune suppression. Increasing evidence has implicated extracellular vesicles (EV), including exosomes in the different processes within the BM. Exosomes are <150 nm sized vesicles secreted by different cell types including MM cells. These vesicles contain protein and RNA cargo which they deliver to the recipient cell. In this way, they have been implicated in MM-related processes including osteolysis, angiogenesis, immune suppression and drug resistance. Targeting exosome secretion could therefore potentially block these different processes. In this review we will summarize the current findings of exosome-related processes in the BM and describe not only the current treatment strategies to counter them but also how exosomes can be harnessed to deliver toxic payloads. Finally, an overview of the different clinical studies which investigate EV cargo as potential MM biomarkers in liquid biopsies will be discussed.

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Characteristics and Clinical Application of Extracellular Vesicle-Derived DNA

Cited by 28 publications

References 161 publications

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Exosomes in multiple myeloma: from bench to bedside

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