Characteristics and applications of a recombinant alkaline serine protease from a novel bacterium Bacillus lehensis

Joshi, Swati; Satyanarayana, T.

doi:10.1016/j.biortech.2012.12.124

Cited by 96 publications

(69 citation statements)

References 31 publications

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“…Proteases activities were assayed according to [7]. The activity was measured by adding substrate casein (1%) prepared in the glycine-NaOH buffer (pH 10) and was incubated at 37°C for 30 min.…”

Section: Enzyme Assaymentioning

confidence: 99%

“…Understanding their biological roles; could allow proteases to be manipulated in many industries, in therapeutics and also academia [1]. Many studies have been carried out on the application of microbial proteases in detergents, foods, pharmaceuticals, leather dehairing, bio-waste disposal, silk degumming, and silver recovery [2,3,4,5,6,7,8]. There are several methods available to improve the yield and activity of proteases such as optimization in fermentation, molecular recombinant and site directed mutagenesis [9].…”

Section: Introductionmentioning

confidence: 99%

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Untitled

2017

JESR

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Abstract:Protease is an enzyme that catalyses the hydrolysis of peptide bond in polypeptide chain and hold a wide range of applications in industry. The aim of this study is to clone and to express several genes encoding proteases from alkalitolerant bacteria Bacillus lehensis strain G1. A total of 13 genes encoding proteases have been selected using bioinformatics approach. These genes were then amplified using polymerase chain reaction (PCR) method. Subsequently, the PCR product was cloned into cloning vector pGEM®T easy and transformed into competent cell E. coli DH5α. The transformants were further verified by sequencing. The positive cloned were subcloned into the expression vector and were then expressed in Luria Bertani medium in the present of IPTG using E. coli BL21. The expressions of recombinant proteases were optimized for several hours at different temperatures, 16-37°C. Furthermore, structural prediction was performed using Modeller v9.18 for BleG1_1940. Each generated model was verified for overall completeness and bias, using PROCHECK, ERRAT, and Verify 3D. The overall quality of the model was relatively good with percentage of Ramachandran plot is 96.3%, PROCHECK is 86.2% and ERRAT score is 95%,.

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Section: Enzyme Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Untitled

2017

JESR

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“…It is also evident the secretion of gelatine degrading alkaline protease by Pseudomonas aeruginosa MCMI B-327 (Kumar et al, 2002). Successful recovery of silver from X-ray films have been reported by alkaline proteases derived from Bacillus subtilis, Conidiobolus coronatus, Streptomyces avermectinus and Bacillus lehensis (Nakiboglu et al, 2001;Shankar et al, 2010;Ahmad et al, 2008;Joshi and Satyanarayana, 2013).…”

Section: Optimization and Validationmentioning

confidence: 99%

“…Among proteases, alkaline proteases are widely used in leather, detergent, food and silk industries (Gessesse, 1997). These proteases, especially from the genus Bacillus having significant stability at high pH and temperature (Yang et al, 2000) and these dominate in leather processing and laundry detergent applications (Haddar et al, 2009;Jain et al, 2012;Nilegaonkar et al, 2007;Shrinivas and Naik, 2011;Joshi and Satyanarayana, 2013). Dehairing alkaline proteases have great applications in leather processing industry.…”

Section: Introductionmentioning

confidence: 99%

Statistical Approach for the Production and Partial Characterization of Alkaline Stable Protease from a Newly Isolated Bacillus sp. IND6 for Silver Recovery

Vijayaragh¹,

Kalaiyaras²,

Prakash³

2015

Research J. of Microbiology

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A low-cost culture medium for the production of bacterial alkaline protease was developed after response surface methodological approach. Central composite design was applied to optimize the concentration of the three significant factors, namely pH, sucrose and yeast extract. A second-order model equation was obtained and then validated experimentally. The model adequacy was highly satisfactory as the coefficient of determination was 0.98. The maximum enzyme production was 2213 U mLG 1 after 72 h of incubation, which showed about fivefold increase in enzyme production than unoptimized medium. The partially purified enzyme showed optimum activity at 50°C at pH 9.0. Crude enzyme exhibited a significant stability with most of the tested commercial laundry detergents. This enzyme removed the gelatin layer of used X-ray film effectively. Considering its promising properties, Bacillus cereus IND6 enzymatic preparations may be considered a potential candidate for detergent applications and silver recovery.

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“…1). Proteases are used in pharmaceutical industry (Kumar et al 2015), food industry for peptide synthesis (Kumar and Bhalla 2005), leather industry for dehairing (Pillai et al 2011;Singh and Bajaj 2017), photographic industry for silver recovery (Joshi and Satyanarayana 2013), detergent industry as an additive for detergent formulation (Giri et al 2011) and in processing of keratin residues (Harde et al 2011). In addition, the proteases are also used for other applications such as contact lens cleaning (Pawar et al 2009), biofilm removal (Leslie 2011), isolation of nucleic acid (Motyan et al 2013), pest control (Joshi and Satyanarayana 2013), degumming of silk (Mahmoodi et al 2010) and selective delignification of hemp (Khan 2013).…”

Section: Introductionmentioning

confidence: 99%

Potential application spectrum of microbial proteases for clean and green industrial production

Singh

Bajaj

2017

Energ. Ecol. Environ.

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Enzymes are the cornerstones of metabolism and constitute the fundamental basis for existence of life. However, recently enzymes are being implicated in diverse industrial processes because of their specific and fast action for efficient bioconversion of substrate to product, and their capability to save raw materials, energy and chemicals for various manufacturing processes. Enzymes are considered as environment-friendly (green) chemicals that may potentially help replacing completely or reducing the usage of hazardous chemicals for industrial processes, thus promising sustainable production and manufacturing. Among various industrial enzymes microbial proteases dominate the world enzyme market due to their multifaceted application potential in varied bioindustries like food, pharmaceutical, textile, photographic, leather and detergent. Promising applications of proteases in agricultural sector for instance may include biocontrol of pests, degumming of silk, selective delignification of hemp and wool processing. However, for successful industrial applications the proteases must be robust enough to suit the process conditions which are generally hostile. Proteases intended for industrial applications must have activity and stability over wide range of temperature and pH extremes for prolonged time periods and even in the presence of various potential enzyme inhibitors. Of various microbial proteases those from Bacillus spp. have got special significance because the latter are known for their ability to produce sturdy enzymes that might have suitability for industrial process conditions. The current article presents an interpretive summary of the recent developments on application potential of proteases for various industries.

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Characteristics and applications of a recombinant alkaline serine protease from a novel bacterium Bacillus lehensis

Cited by 96 publications

References 31 publications

Untitled

Untitled

Statistical Approach for the Production and Partial Characterization of Alkaline Stable Protease from a Newly Isolated Bacillus sp. IND6 for Silver Recovery

Potential application spectrum of microbial proteases for clean and green industrial production

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