Characteristic transport of lactoferrin from the intestinal lumen into the bile via the blood in piglets

Harada, Etsumori; Itoh, Yoshinori; Sitizyo, Kiitiro; Takeuchi, Takashi; Araki, Yoshinori; Kitagawa, Hiroshi

doi:10.1016/s1095-6433(99)00122-1

Cited by 68 publications

(69 citation statements)

References 24 publications

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“…36,37) It has also been reported that oral administration of bLF to mice increased the secretion of anti-bLF IgA and IgG in the intestinal fluid but made no difference in the serum, and it is suggested that bLF could act as an immunostimulating factor of the mucosal immune system. 38) On the other hand, it has been demonstrated that a portion of bLF is transported into blood circulation through the intestine 32,33) as previously noted. In addition, bLF is known to be comparatively robust to digestive enzymes such as pepsin or trypsin.…”

Section: Resultsmentioning

confidence: 76%

“…It is already reported that bLF administered orally to neonatal pigs appeared in blood circulation and was excreted into the bile through entero-hepatic circulation, 33) and in the experiment using adult rats, bLF infused intraduodenally was transported into blood circulation via the lymph pathway. 32) In this study, bLF and L-bLF2 were administered intraduodenally to Wistar rats in order to confirm the bLF absorption to the external jugular vein (300 mg/kg each) and also its transportation to thoracic duct lymph (200 mg/kg each).…”

Section: Resultsmentioning

confidence: 93%

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Liposomalization of Lactoferrin Enhanced It's Anti-inflammatory Effects via Oral Administration

Ishikado

Imanaka

Takeuchi

et al. 2005

Biological & Pharmaceutical Bulletin

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It is known that lactoferrin is one of the functional proteins contained in mammalian milk and that it plays an important role in the immune system. In this study, we prepared multi-lamellar liposomal bovine lactoferrin composed of egg yolk phosphatidylcholine and phytosterol for oral delivery, and examined any resulting anti-inflammatory effects. Oral pretreatment of liposomal lactoferrin exhibited more suppressive effects than did nonliposomal lactoferrin on CCl 4 -induced hepatic injury in rats as well as on lipopolysaccharide-induced TNF-alpha production from mouse peripheral blood mononuclear leukocytes. Further investigation revealed that the liposomalization did not exert influence on the absorbability of lactoferrin to the venous blood or lymph following an intraduodenal administration in rats. Furthermore, there was no significant difference exhibited between the antigenicity of liposomal and non-liposomal lactoferrin, which was measured using the passive cutaneous anaphylaxis reaction following oral sensitization to them in guinea pigs. These results suggest that liposomal lactoferrin might act more effectively than conventional lactoferrin in the intestinal site, which is regarded as an active site of orally administered lactoferrin, although the biological mechanism is not fully understood yet. Consequently we propose that liposomal lactoferrin could be a novel active constituent useful for preventive and therapeutic treatment of inflammatory diseases.

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Section: Resultsmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 93%

Liposomalization of Lactoferrin Enhanced It's Anti-inflammatory Effects via Oral Administration

Ishikado

Imanaka

Takeuchi

et al. 2005

Biological & Pharmaceutical Bulletin

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“…To explain the physiological effects of bLf, it has been analyzed its bodily distribution in rodents and pigs. In newborn pigs, both bLf and bLfcin were absorbed in the small intestine by enterocytes and travelled to the peripheral circulation [32]. Additionally, the transport of bLf through the blood-brain barrier and the blood-cerebrospinal fluid barrier in Wistar rats was demonstrated [33].…”

Section: Introductionmentioning

confidence: 99%

Lactoferrin: A Powerful Antimicrobial Protein Present in Milk

Luna-Castro¹,

Samaniego-Barrón²,

Serrano-Rubio³

et al. 2017

J Adv Dairy Res

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Lactoferrin (Lf) is an iron-chelating glycoprotein present in milk and mucosal secretions, a component of the mammalian innate immune system. Lf is microbiostatic and microbicidal. Lf can reduce the bacterial expression of virulence factors, such as those involved in biofilm production and protease secretion. The high identity among mammalian Lf sequences facilitates its use in human and veterinary medicine. Lf of bovine origin is the principal Lf used due to its commercial availability through purification from milk whey; recombinant Lfs (bovine, human, and porcine) have been used as well. Lf is a stable protein that retains its physicochemical characteristics under gastric pH conditions, and in most cases it is bioactive even after digestion; thus, the incorporation of Lf into diets facilitates its administration to animals. The aim of this review is to examine original research in which the effects of bovine and porcine Lf on pathogens of domestic animals have been demonstrated through in vitro and in vivo assays, with the purpose of ascertaining the benefits that Lf provides in the treatment of infectious diseases.

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“…T h e p e r s o r p t i o n o f macromolecules such as LF and IgG in animal intestines ceases after gut closure [7,28,29], however, it has been reported that LF is persorbed from the intestinal lumen into the blood circulation [13,24,38], and that food antigens and their immune complexes are also persorbed from the intestine into the peripheral blood in adult humans [8,18]. In a previous study, we suggested that luminal bovine serum albumin (BSA) antigen is persorbed by villous columnar epithelial cells at the late apoptotic stage, being mediated by the specific antibody (SpAb) in orally immunized juvenile rats.…”

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confidence: 99%

Persorption Mechanisms of Luminal Antigenic Particulates via Apoptotic Epithelial Cells of Intestinal Villi into Systemic Blood Circulation in Orally Immunized Rats

Yuji

Fujimoto

Miyata

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. The possibility of persorption of prefixed bovine serum albumin-coated sheep erythrocytes (BSA-SEs) from mucous epithelial cells and its mechanisms were investigated in rats orally immunized by BSA for 14 consecutive days. On the day after the final oral immunization, the rats were duodenally perfused by BSA-SEs or non-coated SEs. BSA-SEs were also duodenally perfused in nonimmunized rats. Thirty min after perfusion, BSA-SEs were significantly more engulfed by late-apoptotic-stage villous columnar epithelial cells in the orally immunized rats than those in other experiments. The specific antibody (SpAb) was detected on the surfaces of BSA-SEs in rats with oral immunization. In Peyer's patches of all animals, no SEs reached the follicle-associated epithelium, because of the close attachment of follicle-associated intestinal villi and the thick mucous layer. BSA-SEs were more frequently persorbed into portal blood in the orally immunized rats than in other rats. Small numbers of BSA-SEs or SEs were detected in the systemic blood of all animals. BSA-SEs were also histologically found in the blood vessels of the liver, but not in mesenteric lymph nodes. These findings suggest that sensitized antigenic particulates are taken up by late-apoptotic-stage villous columnar epithelial cells in the small intestine and are finally persorbed into the systemic blood circulation. The uptake of antigenic particulates might be mediated by its luminal SpAb.

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Characteristic transport of lactoferrin from the intestinal lumen into the bile via the blood in piglets

Cited by 68 publications

References 24 publications

Liposomalization of Lactoferrin Enhanced It's Anti-inflammatory Effects via Oral Administration

Liposomalization of Lactoferrin Enhanced It's Anti-inflammatory Effects via Oral Administration

Lactoferrin: A Powerful Antimicrobial Protein Present in Milk

Persorption Mechanisms of Luminal Antigenic Particulates via Apoptotic Epithelial Cells of Intestinal Villi into Systemic Blood Circulation in Orally Immunized Rats

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