With the unique flux pinning characteristics, high temperature superconducting (HTS) bulk can stably levitate above the permanent magnet guideway (PMG). Moreover, owing to its advantages of environmental protection, frictionless and pollution-free, the HTS maglev has shown significant potential in high-speed rail transit. At present, HTS maglev technology is at a critical moment from laboratory research to engineering application. And among the numerous parameters related to its engineering application, the curve negotiation ability determined by the guidance performance of the HTS bulk superconductors is crucial to the safety and stability of its operation. Thus, in this paper, we experimentally investigated the dynamic guidance force of the HTS bulks under different operational conditions including different rotational speeds, maximum lateral displacement (MLD), field cooling height (FCH), and working height (WH) based on a customized HTS maglev dynamic measurement system (SCML-03). In addition, the dynamic guidance force of HTS bulk under long-time operation was studied. It is concluded that FCH, WH, and MLD are intimately associated with the dynamic guidance performance of the HTS bulk, while the rotational speed of the circular PMG has little influence on it. Namely, to improve the dynamic guidance performance of the HTS bulk, reducing the FCH and increasing the WH properly can be regarded as an effective method. Eventually, this research also verifies the long-term lateral stability of the HTS maglev system during dynamic operation.