Characteristic of Biomaterial from Fermentation Product and its Effectto Enzyme Activity of <i>Clarias</i> sp.

Abstract

The physical characteristics of fermented products from restaurant waste food with BIOMS probiotic at a dose of 8% which was incubated for 7 days were investigated in order to study effect of fermentation to the physical characteristic of biomaterial and its effect to enzyme activity of Clarias sp. Fermented products are added to the feed with doses of 0, 10%, 20%, 30% and 40% and physical tests include breaking time, sinking rate test, durability test, and stability test. Furthermore, the feed was given to catfish for 40 days and the enzyme activity (amylase and protease) was analyzed in its digestive tract at the end of the study. It is showed that the addition of fermented products decreased the physical quality of the feed. The best physical quality was found in the control feed with an average value of breaking time of 22.98 hours, an average value of sinking rate of 0.53 cm/s, a durability test of 97.23 %, and a stability test value of 93.53% (10 minutes), 88.8% (30 minutes), and 76.26% (60 minutes). For feed products with doses of fermented products of 10%, 20%, 30%, and 40%, the values ​​of breaking time and sinking rate, durability and stability were lower compared to that of control feed products. Meanwhile, the enzyme activity in the digestive tract of catfish shows that giving a dose of 20% fermented products in the feed resulted in the best composition of amylase and protease enzyme activity of 9.021 units/mL and 275 units/mL. These results indicated that improvements are needed in the fermentation process to produce better physical quality and suitable for catfish feed.