Characteristic Analysis of Peripheral Blood Mononuclear Cell Apoptosis in Coke Oven Workers

Abstract

, immune toxicity and neurotoxicity 3,4) . Immune cell apoptosis is one of the hot spots in immune toxicity research, and peripheral blood mononuclear cell (PBMC) apoptosis can reflect the immune system's stability. Reduced apoptosis would result in excessive proliferation of immune cells and formation of autoimmunity, while increased apoptosis can cause immunodeficiency and formation of immune tolerance 5) . To assess the immune function of coke oven workers, we detected and analyzed the changes in PBMC apoptosis. Subjects and Methods SubjectsThe exposed groups consisted of 129 middle-school educated, 23-49-year-old male coke oven workers employed at least 1 year in the coke plant of a large state-owned company, who were divided into the oven-bottom group (34 subjects), oven-side group (48 subjects) and oven-top group (47 subjects) according to their working sites and environmental monitoring data. A working shift lasted 8 h, and each worker's tasks were relatively constant over 1 yr. The controls were 37 warehouse workers from the raw materials plant at same company who were not occupationally exposed to PAHs from the coking operation and had not visited the coke plant in the previous 3 mo, were The aim of the present study was to determine the peripheral blood mononuclear cell (PBMC) apoptosis in coke oven workers so that we can take effective measures to protect coke oven workers. Methods: The subjects, 129 coke oven workers and 37 warehouse workers (controls), were investigated using a questionnaire to collect information about their age, working years, smoking and drinking habits, vocational history and other general information. The coke oven workers were divided into the oven-bottom group (34), oven-side group (48) and oven-top group (47) according to their working sites and environmental monitoring data. The concentration of benzo[a]pyrene (B[a]P) and the subjects' urinary 1-hydroxypyrene (1-OH-Py) levels were determined by HPLC. Additionally, the PBMCs were separated from blood samples, and the early and late apoptosis rates were determined by flow cytometry. Results: The airborne B[a]P concentrations were 19.5 ± 13.2, 185.9 ± 38.6 and 1,623.5 ± 435.8 ng/m 3 at the bottom, side and top of the oven, respectively, and were higher than in the controls' workplaces 10.2 ± 7.6 ng/m 3 . Urinary 1-OH-Py, indicating the B[a]P's internal exposure level, was significantly higher in the exposed groups than in the controls (p<0.05). Compared with the controls, the coke oven workers' PBMC apoptosis rates were significantly increased and increased in association with the B[a]P level. PBMC apoptosis increased in association with the 1-OH-Py level and coking operation years and decreased in association with years of alcohol consumption. Conclusions: PBMC apoptosis in the coke oven workers was associated with the 1-OH-Py level, coke operation years and

Using Immunotoxicity Information to Improve Cancer Risk Assessment for Polycyclic Aromatic Hydrocarbon Mixtures

“…Although the literature review in this analysis focused on animal studies, several epidemiological studies indicate that exposure to PAH mixtures containing BaP may lead to immunosuppresion in humans. [111][112][113][114][115][116][117][118][119][120] Adverse effects include altered serum immunoglobin and cytokine levels, altered Tand B-cell proliferation and surface antigen expression, increased peripheral blood mononuclear cell apoptosis, altered NK cell numbers, altered phagocytic activity in monocytes, and evidence of DNA damage in peripheral lymphocytes. However, detailed compound-specific and mechanistic data are not available in these human studies due to 1 or more of the following reasons: (1) multiple concurrent chemical exposures, (2) small sample sizes, (3) no measure of exact exposures, and (4) lack of adjustment for potential confounding factors (eg, age).…”

Annexin V FITC conjugated as a radiation toxicity indicator in lymphocytes following radiation overexposure in radiotherapy programs

“…Together with the possibilities of computerized image analysis or flow cytometry, this makes the MN assay more attractive for routine procedures. [ 15 16 ] Theoretically, it enables resolution of doses smaller than the 50-100 mGy usually quoted as the lower limit for detection by scoring chromosome aberrations. [ 6 ] Recently, Boreham et al .…”

“…Results suggest that lymphocytes can be effectively used to predict the prognosis of charged particle therapy, too. [ 14 15 16 17 18 ]…”

Evaluation the Synergistic Effect of High Dose Radiation of Radioiodine on the Immune System Suppressed By Cyclosporine

“…This phenomenon can be observed in the cells especially bone marrow stem cells and Peripheral Blood Mononuclear Cells (PBMCs) after exposure to the radiation (Bordon et al, 2010;Schnarr et al, 2009;Zhang et al, 2012).…”