Characterising the role of the Eucalyptus grandis SND2promoter in secondary cell wall biosynthesis

Botha, Jonathan; Pinard, Desré; Creux, Nicky M.; Hussey, Steven G.; Maritz-Olivier, Christine; Spokevicius, Antanas; Bossinger, Gerd; Mizrachi, Eshchar; Myburg, Alexander Andrew

doi:10.1186/1753-6561-5-s7-p105

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…The overexpression of CcNAC6 in Arabidopsis can significantly increase the lignin content in Arabidopsis plants (Figure 5d,e). The function and energy ratio of the AtSND2 gene are relatively preserved in grasses and woody plants, and the overexpression of AtSND2 in Eucalyptus also increases the thickness of the secondary walls of Eucalyptus fibrocytes [50].…”

Section: Discussionmentioning

confidence: 99%

CcNAC6 Acts as a Positive Regulator of Secondary Cell Wall Synthesis in Sudan Grass (Sorghum sudanense S.)

Huang,

Wu,

Lin

et al. 2024

Plants

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The degree of forage lignification is a key factor affecting its digestibility by ruminants such as cattle and sheep. Sudan grass (Sorghum sudanense S.) is a high-quality sorghum forage, and its lignocellulose is mostly stored in the secondary cell wall. However, the secondary cell wall synthesis mechanism of Sudan grass has not yet been studied in depth. To further study the secondary cell wall synthesis mechanism of Sudan grass using established transcriptome data, this study found that CcNAC6, a homologous gene of Arabidopsis AtSND2, is related to the secondary cell wall synthesis of Sudan grass. Accordingly, we constructed a CcNAC6-overexpressing line of Arabidopsis to investigate the function of the CcNAC6 gene in secondary cell wall synthesis. The results showed that the overexpression of the CcNAC6 gene could significantly increase the lignin content of Arabidopsis. Based on subcellular localization analysis, CcNAC6 is found in the nucleus. In addition, yeast two-hybridization screening showed that CcCP1, associated with secondary cell wall synthesis, can interact with CcNAC6. Therefore, the above results indicate that CcNAC6 has a positive regulatory effect on the secondary cell wall synthesis of Sudan grass, and it is speculated that CcNAC6 may be the main regulator of the secondary cell wall synthesis of Sudan grass through its interaction with another regulatory protein, CcCP1. This study provides a theoretical basis and new genetic resources for the creation of new Sudan grass germplasm with a low lignin content.

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Section: Discussionmentioning

confidence: 99%

CcNAC6 Acts as a Positive Regulator of Secondary Cell Wall Synthesis in Sudan Grass (Sorghum sudanense S.)

Huang,

Wu,

Lin

et al. 2024

Plants

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“…TFs play an important role in these mechanisms by coordinating the expression of defence-related genes in response to invasion (Figure 1). Functional studies on TFs that mediate defence responses in Eucalyptus are limited, but future research could be based on studies of TF function during wood formation (Botha et al 2011;Creux et al 2013;Hussey et al 2011). Epigenetic modifications add another level of complexity to the regulation of host defences (Berr et al 2012).…”

Section: Regulation Of Gene Expression During Defence Responsesmentioning

confidence: 99%

“…Systems biology approaches are increasingly being used to uncover candidate genes for enhanced resistance in plant-pathogen interaction studies (Pritchard and Birch 2011;Windram et al 2014). In Eucalyptus, functional characterisation may involve interaction studies that make use of techniques such as yeast one-hybrid and yeast two-hybrid assays, which can identify interactions between two proteins or between a protein and a DNA sequence (Botha et al 2011;Creux et al 2011).…”

Section: Converting Defence Gene Discovery To Application In Eucalyptus Productionmentioning

confidence: 99%

Uncovering the defence responses of Eucalyptus to pests and pathogens in the genomics age

et al. 2014

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Long-lived tree species are subject to attack by various pests and pathogens during their lifetime. This problem is exacerbated by climate change, which may increase the host range for pathogens and extend the period of infestation by pests. Plant defences may involve preformed barriers or induced resistance mechanisms based on recognition of the invader, complex signalling cascades, hormone signalling, activation of transcription factors and production of pathogenesis-related (PR) proteins with direct antimicrobial or anti-insect activity. Trees have evolved some unique defence mechanisms compared with well-studied model plants, which are mostly herbaceous annuals. The genome sequence of Eucalyptus grandis W. Hill ex Maiden has recently become available and provides a resource to extend our understanding of defence in large woody perennials. This review synthesizes existing knowledge of defence mechanisms in model plants and tree species and features mechanisms that may be important for defence in Eucalyptus, such as anatomical variants and the role of chemicals and proteins. Based on the E. grandis genome sequence, we have identified putative PR proteins based on sequence identity to the previously described plant PR proteins. Putative orthologues for PR-1, PR-2, PR-4, PR-5, PR-6, PR-7, PR-8, PR-9, PR-10, PR-12, PR-14, PR-15 and PR-17 have been identified and compared with their orthologues in Populus trichocarpa Torr. & A. Gray ex Hook and Arabidopsis thaliana (L.) Heynh. The survey of PR genes in Eucalyptus provides a first step in identifying defence gene targets that may be employed for protection of the species in future. Genomic resources available for Eucalyptus are discussed and approaches for improving resistance in these hardwood trees, earmarked as a bioenergy source in future, are considered.

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“…The control mechanisms of lignifications in Eucalyptus have been studied at the level of gene expression regulatory elements. For example, the SND2 promoter (Botha et al 2011) and the cellulose synthase (CesA) promoter (Creux et al , 2013 from Eucalyptus have been functionally characterized. In this study, the promoter region of the 4CL1 gene from E. camaldulensis was isolated and characterized by expression analysis of promoter-GUS fusion construct in transgenic tobacco plants.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

Hue

Hải

et al. 2016

Physiol Mol Biol Plants

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The most important enzyme of the phenylpropanoid pathway, 4-coumarate:coenzyme A ligase (4CL), is encoded by several homologous genes including 4CL1. The 4CL1 promoter is a tissue-specific gene expression element, particularly active in the secondary xylem or older stems. In this study, the 1127 bp 5 0 -upstream region of the 4CL1 coding sequence from Eucalyptus camaldulensis, Euc4CL1, was isolated and characterized. Essential putative cis-elements in the Euc4CL1 promoter included: a TATA-box at -22/-28 position, two CCAAT-boxes at -256/-260 and -277/-281 positions, respectively, an AC-element at -328/-336 and A-boxes at -115/-120 and -990/-995 positions. To investigate the effect of the Euc4CL1 promoter on gene expression, a plant transformation vector, pEuc4CL1p, containing the reporter gene for b-glucuronidase (GUS) under the control of Euc4CL1 promoter was constructed based on the pBI101 backbone and introduced in tobacco plants. Stable expression of the GUS gene in transgenic lines was analysed by a histochemical GUS assay. The results indicated the specific expression of the GUS gene in the stem xylem cells of transgenic tobacco lines was controlled by the Euc4CL1 promoter. The observations suggest the isolated Euc4CL1 promoter is a potential candidate for driving the expression of a foreign gene in plant xylem tissues.

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Characterising the role of the Eucalyptus grandis SND2promoter in secondary cell wall biosynthesis

Cited by 4 publications

References 5 publications

CcNAC6 Acts as a Positive Regulator of Secondary Cell Wall Synthesis in Sudan Grass (Sorghum sudanense S.)

CcNAC6 Acts as a Positive Regulator of Secondary Cell Wall Synthesis in Sudan Grass (Sorghum sudanense S.)

Uncovering the defence responses of Eucalyptus to pests and pathogens in the genomics age

Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

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