Characterisation of uropathogenic Escherichia coli from children with urinary tract infection in different countries

Abstract

Uropathogenic Escherichia coli (UPEC) carry many virulence factors, including those involved in long-term survival in the urinary tract. However, their prevalence and role among UPEC causing urinary tract infection (UTI) in children is not well studied. To further understand the virulence characteristics of these bacteria, we investigated the prevalence of antibiotic resistance, antigen 43 genes, curli and cellulose among UPEC in children from different countries. Isolates (n = 337) from five countries were tested for antibiotic susceptibility, phylogenetic groups, prevalence of flu, fluA(CFT073), fluB(CFT073), curli and cellulose. High prevalence of multidrug resistance and extended spectrum beta lactamase production was found among Iranian and Vietnamese isolates. Resistance was associated with phylogenetic group D while group B2 was associated with fluA(CFT073) and fluB(CFT073). Fewer Iranian isolates carried fluA(CFT073), curli and cellulose. fluB(CFT073) was most prevalent among Slovak isolates. Ampicillin and amoxicillin/clavulanic acid resistance was prevalent among fluA(CFT073)- and fluB(CFT073)-positive Australian, Iranian and Swedish isolates. Lack of curli and cellulose was associated with resistance among Vietnamese isolates. We conclude that major differences exist in the prevalence of antibiotic resistance among UPEC from different countries. Associations observed between resistance and virulence factors may, in different ways, promote the long-term survival of UPEC in the urinary tract.

Uropathogenic Escherichia coli Isolates from Pregnant Women in Different Countries

“…Species identification was first performed at the hospitals at which samples were collected. Thereafter, E. coli identification was confirmed using the Vitek 2 GramNegative Identification Card (bioMérieux, Marcy l'Etoile, France), as previously described (21). Vitek screening and all subsequent tests in this study were performed at the Department of Clinical Microbiology, Karolinska University Hospital.…”

“…PCR amplification of virulence factor genes. A modified boiling method was used to extract whole-cell DNA from E. coli isolates (21). One or two colonies of bacteria grown on blood agar were suspended in 100 l sterile deionized water and boiled at 99°C.…”

Caracterização de Escherichia coli uropatogênicas isoladas de crianças com infecção urinária.

“…O contrário foi observado no Hospital Ana Rosa onde 72% das amostras positivas foram obtidas de pacientes do sexo feminino e apenas 28% do masculino (LOURENÇO et al, 2005). Estudos semelhantes em outros países como Índia (64%), México (68,9%), Iran (65,2% -85,2%), Canadá (56,3%), Itália (53%) e um estudo multicentrico envolvendo vários países (Iran, Vietnã, Austrália, Eslováquia e Suécia -65%) mostraram que a maior parte dos pacientes com ITU é do sexo feminino (EMAMGHORASHI et al, 2011;JADHAV et al, 2011;KASHEF et al, 2010;KWAN;ONYETT, 2008;MOLINA-LÓPEZ et al, 2011;RAMOS et al, 2011).…”

“…Outros Enterococcus spp. Outros Odds Ratio IC 95% 13 602 GUIDONI et al, 2008;GÜNDOĞDU et al, 2011;JADAHAV et al, 2011;MOLINA-LÓPEZ et al, 2011;OLIVEIRA et al, 2011;RAMOS et al, 2011;UPTON et al, 1999). Todavia, foi também observado em alguns desses estudos uma baixa resistência a nitrofurantoína como na Grécia (2,2%), Canadá (2,7%), Iran (3,2%), São Paulo (6%), Rio de Janeiro (3%), México (5,5%) e Itália (<1%) valores esses muito menores que o encontrado em nosso estudo.…”

“…grupo filogenético, mesmo dentro de um mesmo estudo. (GÜNDOĞDU et al, 2011;RAMOS et al, 2011;RAMOS et al 2011;ABDALLAH et al, 2011). Ao contrário dos resultados observados nesses trabalhos, um estudo realizado com pacientes portadores de desordem neurológica e bexiga neurogênica, mostrou que as E. coli uropatogênicas provenientes desses pacientes se distribuíram igualmente entre os quatro grupos filogenéticos (MLADIN et al, 2009).…”