Characterisation of two novel bacteriocin-like substances produced by Bacillus amyloliquefaciens ELI149 with broad-spectrum antimicrobial activity

Salazar, Francisco; Ortíz, Aurelio; Sansinenea, Estibaliz

doi:10.1016/j.jgar.2017.08.008

Cited by 41 publications

(23 citation statements)

References 16 publications

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“…Nevertheless, due to the production of bacteriocins, Bacillus spp. exhibit a broad-spectrum of antibacterial activity [ 24 ]. Some reports identify bacteriocins and bacteriocin-like substances (BLSs) (amylolysin, amysin, subtilin, subtilosin A, subtilosin B, thuricin) isolated from various Bacillus spp., including B. amyloliquefaciens, B. subtilis, B. thuringiensis, B. cereus, and B. coagulans [ 25 ].…”

Section: Mechanisms Of Biological Controlmentioning

confidence: 99%

The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

2020

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Bacillus spp. produce a variety of compounds involved in the biocontrol of plant pathogens and promotion of plant growth, which makes them potential candidates for most agricultural and biotechnological applications. Bacilli exhibit antagonistic activity by excreting extracellular metabolites such as antibiotics, cell wall hydrolases, and siderophores. Additionally, Bacillus spp. improve plant response to pathogen attack by triggering induced systemic resistance (ISR). Besides being the most promising biocontrol agents, Bacillus spp. promote plant growth via nitrogen fixation, phosphate solubilization, and phytohormone production. Antagonistic and plant growth-promoting strains of Bacillus spp. might be useful in formulating new preparations. Numerous studies of a wide range of plant species revealed a steady increase in the number of Bacillus spp. identified as potential biocontrol agents and plant growth promoters. Among different mechanisms of action, it remains unclear which individual or combined traits could be used as predictors in the selection of the best strains for crop productivity improvement. Due to numerous factors that influence the successful application of Bacillus spp., it is necessary to understand how different strains function in biological control and plant growth promotion, and distinctly define the factors that contribute to their more efficient use in the field.

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Section: Mechanisms Of Biological Controlmentioning

confidence: 99%

The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

2020

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“…Bacteriocins are a highly diverse group of antimicrobial peptides. They have variations in molecular weight, inhibitory spectrum, mode of action, mechanism of biosynthesis and externalization, and self-protection mechanism (Salazar et al, 2017 ). They are part of the inherent defense system of bacteria and play other roles such as niche colonization, direct killing of competing strains and signaling (cross-talk and quorum sensing) within bacterial communities (Dobson et al, 2012 ; Inglis et al, 2013 ; Yang et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Pentocin MQ1: A Novel, Broad-Spectrum, Pore-Forming Bacteriocin From Lactobacillus pentosus CS2 With Quorum Sensing Regulatory Mechanism and Biopreservative Potential

Wayah

Philip

2018

Front. Microbiol.

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Micrococcus luteus, Listeria monocytogenes, and Bacillus cereus are major food-borne pathogenic and spoilage bacteria. Emergence of antibiotic resistance and consumer demand for foods containing less of chemical preservatives led to a search for natural antimicrobials. A study aimed at characterizing, investigating the mechanism of action and regulation of biosynthesis and evaluating the biopreservative potential of pentocin from Lactobacillus pentosus CS2 was conducted. Pentocin MQ1 is a novel bacteriocin isolated from L. pentosus CS2 of coconut shake origin. The purification strategy involved adsorption-desorption of bacteriocin followed by RP-HPLC. It has a molecular weight of 2110.672 Da as determined by MALDI-TOF mass spectrometry and a molar extinction value of 298.82 M−1 cm−1. Pentocin MQ1 is not plasmid-borne and its biosynthesis is regulated by a quorum sensing mechanism. It has a broad spectrum of antibacterial activity, exhibited high chemical, thermal and pH stability but proved sensitive to proteolytic enzymes. It is potent against M. luteus, B. cereus, and L. monocytogenes at micromolar concentrations. It is quick-acting and exhibited a bactericidal mode of action against its targets. Target killing was mediated by pore formation. We report for the first time membrane permeabilization as a mechanism of action of the pentocin from the study against Gram-positive bacteria. Pentocin MQ1 is a cell wall-associated bacteriocin. Application of pentocin MQ1 improved the microbiological quality and extended the shelf life of fresh banana. This is the first report on the biopreservation of banana using bacteriocin. These findings place pentocin MQ1 as a potential biopreservative for further evaluation in food and medical applications.

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“…Bacterial culture filtrate extract was obtained in the solution of ethyl acetate and methanol (1:1 v/v), which was passed through the BCFE-trapped XAD 16 resin. XAD resin has an excellent capacity to enrich different biological molecules including phenolic compounds, flavonoids, peptides, antimicrobial, pharmaceuticals, etc., and has been extensively used for the selective extraction of biomolecules from complex microbial culture media (Liu et al, 2016;Salazar et al, 2017;González-Menéndez et al, 2019) and other biological mixtures, such as adlay bran (Yang et al, 2016), apple skin Wang et al (2020), Glycyrrhiza leaves (Dong et al, 2015), and wheat gluten hydrolysates (Yang et al, 2018), as well as extraction of pharmaceuticals in wastewater (Yu and Wu, 2011). Thus, to achieve maximal entrapment of microbial secretions and minimize the interference of residual medium impurities in the crude biomolecule mixture, we specifically used XAD-16 resin to trap the biomolecules secreted by Nocardioides sp.…”

Section: Quantification Of Iaa and Sa In Bcfementioning

confidence: 99%

Mitigation of Salinity Stress in Wheat Seedlings Due to the Application of Phytohormone-Rich Culture Filtrate Extract of Methylotrophic Actinobacterium Nocardioides sp. NIMMe6

et al. 2020

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Salinity stress is an important plant growth limiting factor influencing crop productivity negatively. Microbial interventions for salinity stress mitigation have invited significant attention due to the promising impacts of interactive associations on the intrinsic mechanisms of plants. We report the impact of microbial inoculation of a halotolerant methylotrophic actinobacterium (Nocardioides sp. NIMMe6; LC140963) and seed coating of its phytohormone-rich bacterial culture filtrate extract (BCFE) on wheat seedlings grown under saline conditions. Different plant-growth-promoting (PGP) attributes of the bacterium in terms of its growth in N-limiting media and siderophore and phytohormone [indole-3-acetic acid (IAA) and salicylic acid] production influenced plant growth positively. Microbial inoculation and priming with BCFE resulted in improved germination (92% in primed seeds at 10 dS m −1), growth, and biochemical accumulation (total protein 42.01 and 28.75 mg g −1 in shoot and root tissues at 10 dS m −1 in BCFE-primed seeds) and enhanced the activity level of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase) to confer stress mitigation. Biopriming with BCFE proved impactful. The BCFE application has further influenced the overexpression of defense-related genes in the seedlings grown under salinity stress condition. Liquid chromatography-mass spectrometrybased characterization of the biomolecules in the BCFE revealed quantification of salicylate and indole-3-acetate (Rt 4.978 min, m/z 138.1 and 6.177 min, 129.1), respectively. The high tolerance limit of the bacterium to 10% NaCl in the culture media suggested its possible survival and growth under high soil salinity condition as microbial inoculant. The production of a high quantity of IAA (45.6 µg ml −1 of culture filtrate) by the bacterium reflected its capability to not only support plant growth under salinity condition but also mitigate stress due to the impact of phytohormone as defense mitigators. The study suggested that although microbial inoculation offers stress mitigation in plants, the phytohormone-rich BCFE from Nocardioides sp. NIMMe6 has potential implications for defense against salinity stress in wheat.

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Characterisation of two novel bacteriocin-like substances produced by Bacillus amyloliquefaciens ELI149 with broad-spectrum antimicrobial activity

Cited by 41 publications

References 16 publications

The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

Pentocin MQ1: A Novel, Broad-Spectrum, Pore-Forming Bacteriocin From Lactobacillus pentosus CS2 With Quorum Sensing Regulatory Mechanism and Biopreservative Potential

Mitigation of Salinity Stress in Wheat Seedlings Due to the Application of Phytohormone-Rich Culture Filtrate Extract of Methylotrophic Actinobacterium Nocardioides sp. NIMMe6

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