Characterisation of transcriptional responses to dioxins and dioxin-like contaminants in roach ( Rutilus rutilus ) using whole transcriptome analysis

Brinkmann, Markus; Koglin, Sven; Eisner, Bryanna; Wiseman, Steve; Hecker, Markus; Eichbaum, Kathrin; Thalmann, Beat; Buchinger, Sebastian; Reifferscheid, Georg; Hollert, Henner

doi:10.1016/j.scitotenv.2015.09.087

Cited by 32 publications

(31 citation statements)

References 100 publications

(101 reference statements)

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“…3), which may indicate an increased response to oxidative stress. In the study of Brinkmann et al [11], a significantly lesser abundance of transcripts of sod , pkcd , pygl, and tfd was observed following injection of TCDD, while the abundance of transcripts of abcc9 and pc was significantly greater. The results of these other genes investigated in the present study do not currently enable us to draw reliable conclusions on any effects on higher levels of biological organization linked to the respective genes.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, exposure of roach to re-suspended samples of sediments from three locations along the Elbe and Rhine rivers contaminated with different concentrations of DLCs was assessed by quantifying concentrations of PAH metabolites in bile fluid, expression of cyp1a and EROD activity as biomarkers of activation of the AhR, and expression of several genes that were found to be dioxin-responsive in a previous study [11]. The surveyed genes encode for proteins that are part of important cellular processes, including biotransformation of xenobiotics, energy metabolism, and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Primers for quantitative real-time PCR (qPCR) of selected transcripts (Table 2) were obtained from Eurofins Genomics (Ebersberg, Germany). The primers were designed using a database of the transcriptome of roach liver generated previously [11]. PCR products of cDNA samples were separated on 1% agarose gels along with a DNA size standard (New England Bioloabs, Frankfurt am Main, Germany) to confirm single PCR amplicons with the desired length.…”

Section: Methodsmentioning

confidence: 99%

“…In other studies, the uptake of contaminants such as PCBs, PAHs, and heavy metals, and the induction of several biomarkers of exposure to these chemicals, including glutathione- S -transferase (GST) and 7-ethoxyresorufin- O -deethylase (EROD), have been investigated [16, 17, 34, 47, 65]. However, a very limited number of studies report the results of laboratory exposures of roach to single substances [11, 13, 14, 70]. …”

Section: Introductionmentioning

confidence: 99%

“…Analyses in exposed fish comprised of measures of the uptake of PAHs by means of high performance liquid chromatography analysis of bile liquid with fluorescence detection (HPLC-F). In addition, the effects of sediment exposure on the expression of a number of genes that were selected based on results of a previous study to characterize the effects of selected DLCs in roach by means of next generation high throughput sequencing of RNA (RNAseq) were investigated [11]. With this selection of genes, several important cellular pathways were surveyed: (a) phase I metabolism of xenobiotics and multidrug resistance (MDR) by quantifying expression of cytochrome p450 1a ( cyp1a ) [69] and the ATP - binding cassette transporter c9 ( abcc9) that encodes for the MDR- and potassium channel-regulating protein ABCC9 [15, 46]; (b) energy metabolism by quantifying expression of pyruvate carboxylase ( pc ) and glycogen phosphorylase ( pygl ), the products of which are important for synthesis of oxaloacetate for the citric acid cycle and gluconeogenesis (PC, [2, 31, 66] and liberation of glucose from glycogen, respectively (PYGL, [56, 57]; (c) apoptotic processes by measuring the expression of protein kinase c delta ( pkcd ) that regulates the expression and function of many apoptosis-related proteins [7, 12, 44, 49, 50]; (d) the response to oxidative stress by quantifying expression of extracellular superoxide dismutase ( sod ).…”

Section: Introductionmentioning

confidence: 99%

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Toward understanding the impacts of sediment contamination on a native fish species: transcriptional effects, EROD activity, and biliary PAH metabolites

Koglin

Kammann²,

Eichbaum

et al. 2016

Environ Sci Eur

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BackgroundBoth frequency and intensity of flood events are expected to increase as a result of global climate change in the upcoming decades, potentially resulting in increased re-suspension of sediments in fluvial systems. Contamination of these re-suspended sediments with legacy contaminants, including dioxins and dioxin-like compounds (DLCs), as well as polycyclic aromatic hydrocarbons (PAHs) is of great ecotoxicological concern. DLCs, and to some extent also PAHs, exhibit their toxicity through activation of the aryl hydrocarbon receptor (AhR). However, interactions of DLCs with pathways other than those known to be mediated through the AhR are not fully understood to date.MethodsThis study aimed to investigate molecular and biochemical effects in roach (Rutilus rutilus) during a 10 days exposure to suspensions of three natural sediments that differed in the level of DLC contamination. Concentrations of biliary PAH metabolites and hepatic 7-ethoxyresorufin-O-deethylase activity were quantified in exposed fish. Furthermore, the abundance of transcripts of several genes related to energy metabolism, response to oxidative stress, and apoptosis, as well as cytochrome P450 1A (cyp1a) was quantified.ResultsBiliary PAH metabolites and activation of the AhR were confirmed as suitable early warning biomarkers of exposure to suspended sediments containing DLCs and PAHs that corresponded well with analytically determined concentrations of those contaminants. Although the abundances of transcripts of superoxide dismutase (sod), protein kinase c delta (pkcd), and ATP-binding cassette transporter c9 (abcc9) were altered by the treatment compared with unexposed control fish, none of these showed a time- or concentration-dependent response. The abundance of transcripts of pyruvate carboxylase (pc) and transferrin variant d (tfd) remained unaltered by the treatments.ConclusionsWe have shown that contaminated sediments can become a risk for fish during re-suspension events (e.g., flooding and dredging). We have also demonstrated that roach, which are native to most European freshwater systems, are suitable sentinel species due to their great sensitivity and ecological relevance. Roach may be particularly suitable in future field studies to assess the toxicological concerns associated with the release of DLCs and PAHs during sediment re-suspension.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Toward understanding the impacts of sediment contamination on a native fish species: transcriptional effects, EROD activity, and biliary PAH metabolites

Koglin

Kammann²,

Eichbaum

et al. 2016

Environ Sci Eur

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Expression of aryl hydrocarbon receptor–regulated genes and superoxide dismutase in the Antarctic eelpout Pachycara brachycephalum exposed to benzo[a]pyrene

Strobel

Mark

Segner

et al. 2018

Enviro Toxic and Chemistry

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The aryl hydrocarbon receptor (AhR) pathway mediates many, if not all, responses of fish to dioxin-like compounds. The Southern Ocean is progressively exposed to increasing concentrations of anthropogenic pollutants. Antarctic fish are known to accumulate those pollutants, yet nothing is known about their capability to induce chemical biotransformation via the AhR pathway. The objective of the present study was to investigate whether Antarctic eelpout, Pachycara brachycephalum, respond to anthropogenic pollutants by activation of the AhR and its target gene cytochrome P4501A (CYP1A), and of superoxide dismutase (SOD), which served as a representative for oxidative stress. We exposed P. brachycephalum to 10 and 100 mg benzo[a]pyrene (BaP)/kg body weight for 10 d and measured the expression of AhR, CYP1A, and SOD in liver tissue via quantitative polymerase chain reaction. We identified two distinct AhR isoforms in the liver of P. brachycephalum. Antarctic eelpout responded to both BaP exposures by an up-regulation of AhR and SOD, and by a particularly strong induction of CYP1A expression, which remained high until day 10 of the exposure time. Our data suggest that P. brachycephalum possesses the potential to up-regulate xenobiotic biotransformation pathways, at least at the gene expression level. The time course of the AhR and CYP1A response points to an efficient but slow xenobiotics metabolism. Moreover, BaP exposure could include adverse effects such as oxidative stress. Environ Toxicol Chem 2018;37:1487-1495. © 2018 SETAC.

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Alkylation of Benz[a]anthracene Affects Toxicity to Early–Life Stage Zebrafish and In Vitro Aryl Hydrocarbon Receptor 2 Transactivation in a Position‐Dependent Manner

Dubiel

Green

Raza

et al. 2022

Enviro Toxic and Chemistry

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Polycyclic aromatic hydrocarbons (PAHs) are structurally diverse organic chemicals that can have adverse effects on the health of fishes through activation of aryl hydrocarbon receptor 2 (AhR2). They are ubiquitous in the environment, but alkyl PAHs are more abundant in some environmental matrices. However, relatively little is known regarding the effects of alkylation on the toxicity of PAHs to fishes in vivo and how this relates to potency for activation of AhR2 in vitro. Therefore, the objectives of the present study were to determine the toxicity of benz[a]anthracene and three alkylated homologs representing various alkylation positions to early life stages of zebrafish (Danio rerio) and to assess the potency of each for activation of the zebrafish AhR2 in a standardized in vitro AhR transactivation assay. Exposure of embryos to each of the PAHs caused a dose‐dependent increase in mortality and malformations characteristic of AhR2 activation. Each alkyl homolog had in vivo toxicities and in vitro AhR2 activation potencies different from those of the parent PAH in a position‐dependent manner. However, there was no statistically significant linear relationship between responses measured in these assays. The results suggest a need for further investigation into the effect of alkylation on the toxicity of PAHs to fishes and greater consideration of the contribution of alkylated homologs in ecological risk assessments. Environ Toxicol Chem 2022;41:1993–2002. © 2022 SETAC

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Characterisation of transcriptional responses to dioxins and dioxin-like contaminants in roach ( Rutilus rutilus ) using whole transcriptome analysis

Cited by 32 publications

References 100 publications

Toward understanding the impacts of sediment contamination on a native fish species: transcriptional effects, EROD activity, and biliary PAH metabolites

Toward understanding the impacts of sediment contamination on a native fish species: transcriptional effects, EROD activity, and biliary PAH metabolites

Expression of aryl hydrocarbon receptor–regulated genes and superoxide dismutase in the Antarctic eelpout Pachycara brachycephalum exposed to benzo[a]pyrene

Alkylation of Benz[a]anthracene Affects Toxicity to Early–Life Stage Zebrafish and In Vitro Aryl Hydrocarbon Receptor 2 Transactivation in a Position‐Dependent Manner

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