Characterisation of the transcriptome of Aphelenchoides besseyi and identification of a GHF 45 cellulase

Kikuchi, T.; Cock, Peter; Helder, J.; Jones, John T.

doi:10.1163/15685411-00002748

Cited by 18 publications

(28 citation statements)

References 35 publications

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“…On the basis of a draft genome sequence, the pine wood nematode B. xylophilus is predicted to produce up to 11 distinct GHF45 cellulases, but no GHF5 cellulase-encoding sequences were found (46). Two independent transcriptome analyses of a representative of the plant-parasitic Aphelenchoididae, A. besseyi, the causal agent of white tip in rice, revealed other GHF45 cellulases (45,76). Also in the case of A. besseyi, neither of the two transcriptomes harbored any evidence for the presence of GHF5 cellulases.…”

Section: Plant Cell Wall-degrading Enzymes Underpin the Independent Ementioning

confidence: 99%

Evolution of Plant Parasitism in the Phylum Nematoda

Quist¹,

Smant²,

Helder³

2015

Annu. Rev. Phytopathol.

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Within the species-rich and trophically diverse phylum Nematoda, at least four independent major lineages of plant parasites have evolved, and in at least one of these major lineages plant parasitism arose independently multiple times. Ribosomal DNA data, sequence information from nematode-produced, plant cell wall-modifying enzymes, and the morphology and origin of the style(t), a protrusible piercing device used to penetrate the plant cell wall, all suggest that facultative and obligate plant parasites originate from fungivorous ancestors. Data on the nature and diversification of plant cell wall-modifying enzymes point at multiple horizontal gene transfer events from soil bacteria to bacterivorous nematodes resulting in several distinct lineages of fungal or oomycete-feeding nematodes. Ribosomal DNA frameworks with sequence data from more than 2,700 nematode taxa combined with detailed morphological information allow for explicit hypotheses on the origin of agronomically important plant parasites, such as root-knot, cyst, and lesion nematodes.

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Section: Plant Cell Wall-degrading Enzymes Underpin the Independent Ementioning

confidence: 99%

Evolution of Plant Parasitism in the Phylum Nematoda

Quist¹,

Smant²,

Helder³

2015

Annu. Rev. Phytopathol.

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“…besseyi transcriptome were obtained from Kikuchi et al and Wang et al [17,18], b Data of B . xylophilus whole genome were obtained from Kikuchi et al [16],…”

Section: Resultsmentioning

confidence: 99%

“…Kikuchi et al performed a small-scale transcriptome sequencing project for A . besseyi , obtaining 5,804 transcripts with a mean length of 828 bp [17]. Among nematodes that parasitize above-ground plant parts, Kikuchi et al conducted genome sequencing of B .…”

Section: Discussionmentioning

confidence: 99%

“…Plant parasitic nematodes produce enzymes that break down the cell wall, the primary barrier faced by plant parasite nematodes to invade the plant cytoplasm. Since the first plant cell wall-degrading enzymes (carbohydrate-active enzymes, CAZymes) from nematodes were characterized in cyst nematodes [19], plant cell wall enzymes have been identified in many plant parasitic nematodes, including Aphelenchoides , Bursaphelenchus , Ditylenchus , Globodera , Heterodera , and Meloidogyne [16,17,20]. Some CAZymes that are secreted by parasitic nematodes of the below-ground parts of plants have been predicted to be involved in enabling nematode access to the roots and feeding on host cells [21].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis of the Chrysanthemum Foliar Nematode, Aphelenchoides ritzemabosi (Aphelenchida: Aphelenchoididae)

Wang

et al. 2016

PLoS ONE

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The chrysanthemum foliar nematode (CFN), Aphelenchoides ritzemabosi, is a plant parasitic nematode that attacks many plants. In this study, a transcriptomes of mixed-stage population of CFN was sequenced on the Illumina HiSeq 2000 platform. 68.10 million Illumina high quality paired end reads were obtained which generated 26,817 transcripts with a mean length of 1,032 bp and an N50 of 1,672 bp, of which 16,467 transcripts were annotated against six databases. In total, 20,311 coding region sequences (CDS), 495 simple sequence repeats (SSRs) and 8,353 single-nucleotide polymorphisms (SNPs) were predicted, respectively. The CFN with the most shared sequences was B. xylophilus with 16,846 (62.82%) common transcripts and 10,543 (39.31%) CFN transcripts matched sequences of all of four plant parasitic nematodes compared. A total of 111 CFN transcripts were predicted as homologues of 7 types of carbohydrate-active enzymes (CAZymes) with plant/fungal cell wall-degrading activities, fewer transcripts were predicted as homologues of plant cell wall-degrading enzymes than fungal cell wall-degrading enzymes. The phylogenetic analysis of GH5, GH16, GH43 and GH45 proteins between CFN and other organisms showed CFN and other nematodes have a closer phylogenetic relationship. In the CFN transcriptome, sixteen types of genes orthologues with seven classes of protein families involved in the RNAi pathway in C. elegans were predicted. This research provides comprehensive gene expression information at the transcriptional level, which will facilitate the elucidation of the molecular mechanisms of CFN and the distribution of gene functions at the macro level, potentially revealing improved methods for controlling CFN.

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“…Bursaphelenchus belongs to the family Parasitaphelenchidae, and recently a cellulase from its sister family, Aphelenchoididae, was isolated and characterized. A small-scale analysis of the transcriptome of the foliar nematode Aphelenchoides besseyi resulted in the discovery of another GHF45 cellulase that is produced and presumably secreted (as the core protein is preceded by a predicted signal peptide for secretion) by this nematode species (Kikuchi et al 2014). So far each plant parasite lineage was thought to be characterized by the production of cellulases from a single GH family.…”

Section: The Role Of Lateral Gene Transfer In the Evolution Of Plant mentioning

confidence: 99%

Phytopathogenic Nematodes

Helder¹,

Vervoort²,

Megen³

et al. 2014

Principles of Plant-Microbe Interactions

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Soil is teeming with life, and rhizosphere soil is even more densely inhabited than bulk soil. In terms of biomass, bacteria and fungi are dominant groups, whereas nematodes (roundworms) are the most abundant Metazoans. Bulk soil, soil not directly affected by living plant roots, typically harbours around 2000-4000 nematodes per 100 g, while in the rhizosphere these numbers should be multiplied by a factor 3-5. This difference is not only explained by a higher density of plant parasites, as also bacterivorous and fungivorous nematodes benefit from the local boost of the bacterial and fungal community. Most nematodes feeding on higher plants are obligatory parasites. In this chapter four independent lineages of plant-parasitic nematodes are discussed. Facultative plant parasites often occupy basal positions

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Characterisation of the transcriptome of Aphelenchoides besseyi and identification of a GHF 45 cellulase

Cited by 18 publications

References 35 publications

Evolution of Plant Parasitism in the Phylum Nematoda

Evolution of Plant Parasitism in the Phylum Nematoda

Transcriptome Analysis of the Chrysanthemum Foliar Nematode, Aphelenchoides ritzemabosi (Aphelenchida: Aphelenchoididae)

Phytopathogenic Nematodes

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